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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MUC2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MUC2 SMUC
Sequence Length (AA) Molecular Weight (Da)
5179 540300
Protein Name
Mucin-2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGLPLARLAA VCLALSLAGG SELQTEGRTR YHGRNVCSTW GNFHYKTFDG
60 70 80 90 100
DVFRFPGLCD YNFASDCRGS YKEFAVHLKR GPGQAEAPAG VESILLTIKD
110 120 130 140 150
DTIYLTRHLA VLNGAVVSTP HYSPGLLIEK SDAYTKVYSR AGLTLMWNRE
160 170 180 190 200
DALMLELDTK FRNHTCGLCG DYNGLQSYSE FLSDGVLFSP LEFGNMQKIN
210 220 230 240 250
QPDVVCEDPE EEVAPASCSE HRAECERLLT AEAFADCQDL VPLEPYLRAC
260 270 280 290 300
QQDRCRCPGG DTCVCSTVAE FSRQCSHAGG RPGNWRTATL CPKTCPGNLV
310 320 330 340 350
YLESGSPCMD TCSHLEVSSL CEEHRMDGCF CPEGTVYDDI GDSGCVPVSQ
360 370 380 390 400
CHCRLHGHLY TPGQEITNDC EQCVCNAGRW VCKDLPCPGT CALEGGSHIT
410 420 430 440 450
TFDGKTYTFH GDCYYVLAKG DHNDSYALLG ELAPCGSTDK QTCLKTVVLL
460 470 480 490 500
ADKKKNAVVF KSDGSVLLNQ LQVNLPHVTA SFSVFRPSSY HIMVSMAIGV
510 520 530 540 550
RLQVQLAPVM QLFVTLDQAS QGQVQGLCGN FNGLEGDDFK TASGLVEATG
560 570 580 590 600
AGFANTWKAQ STCHDKLDWL DDPCSLNIES ANYAEHWCSL LKKTETPFGR
610 620 630 640 650
CHSAVDPAEY YKRCKYDTCN CQNNEDCLCA ALSSYARACT AKGVMLWGWR
660 670 680 690 700
EHVCNKDVGS CPNSQVFLYN LTTCQQTCRS LSEADSHCLE GFAPVDGCGC
710 720 730 740 750
PDHTFLDEKG RCVPLAKCSC YHRGLYLEAG DVVVRQEERC VCRDGRLHCR
760 770 780 790 800
QIRLIGQSCT APKIHMDCSN LTALATSKPR ALSCQTLAAG YYHTECVSGC
810 820 830 840 850
VCPDGLMDDG RGGCVVEKEC PCVHNNDLYS SGAKIKVDCN TCTCKRGRWV
860 870 880 890 900
CTQAVCHGTC SIYGSGHYIT FDGKYYDFDG HCSYVAVQDY CGQNSSLGSF
910 920 930 940 950
SIITENVPCG TTGVTCSKAI KIFMGRTELK LEDKHRVVIQ RDEGHHVAYT
960 970 980 990 1000
TREVGQYLVV ESSTGIIVIW DKRTTVFIKL APSYKGTVCG LCGNFDHRSN
1010 1020 1030 1040 1050
NDFTTRDHMV VSSELDFGNS WKEAPTCPDV STNPEPCSLN PHRRSWAEKQ
1060 1070 1080 1090 1100
CSILKSSVFS ICHSKVDPKP FYEACVHDSC SCDTGGDCEC FCSAVASYAQ
1110 1120 1130 1140 1150
ECTKEGACVF WRTPDLCPIF CDYYNPPHEC EWHYEPCGNR SFETCRTING
1160 1170 1180 1190 1200
IHSNISVSYL EGCYPRCPKD RPIYEEDLKK CVTADKCGCY VEDTHYPPGA
1210 1220 1230 1240 1250
SVPTEETCKS CVCTNSSQVV CRPEEGKILN QTQDGAFCYW EICGPNGTVE
1260 1270 1280 1290 1300
KHFNICSITT RPSTLTTFTT ITLPTTPTSF TTTTTTTTPT SSTVLSTTPK
1310 1320 1330 1340 1350
LCCLWSDWIN EDHPSSGSDD GDREPFDGVC GAPEDIECRS VKDPHLSLEQ
1360 1370 1380 1390 1400
HGQKVQCDVS VGFICKNEDQ FGNGPFGLCY DYKIRVNCCW PMDKCITTPS
1410 1420 1430 1440 1450
PPTTTPSPPP TTTTTLPPTT TPSPPTTTTT TPPPTTTPSP PITTTTTPLP
1460 1470 1480 1490 1500
TTTPSPPIST TTTPPPTTTP SPPTTTPSPP TTTPSPPTTT TTTPPPTTTP
1510 1520 1530 1540 1550
SPPMTTPITP PASTTTLPPT TTPSPPTTTT TTPPPTTTPS PPTTTPITPP
1560 1570 1580 1590 1600
TSTTTLPPTT TPSPPPTTTT TPPPTTTPSP PTTTTPSPPT ITTTTPPPTT
1610 1620 1630 1640 1650
TPSPPTTTTT TPPPTTTPSP PTTTPITPPT STTTLPPTTT PSPPPTTTTT
1660 1670 1680 1690 1700
PPPTTTPSPP TTTTPSPPIT TTTTPPPTTT PSSPITTTPS PPTTTMTTPS
1710 1720 1730 1740 1750
PTTTPSSPIT TTTTPSSTTT PSPPPTTMTT PSPTTTPSPP TTTMTTLPPT
1760 1770 1780 1790 1800
TTSSPLTTTP LPPSITPPTF SPFSTTTPTT PCVPLCNWTG WLDSGKPNFH
1810 1820 1830 1840 1850
KPGGDTELIG DVCGPGWAAN ISCRATMYPD VPIGQLGQTV VCDVSVGLIC
1860 1870 1880 1890 1900
KNEDQKPGGV IPMAFCLNYE INVQCCECVT QPTTMTTTTT ENPTPPTTTP
1910 1920 1930 1940 1950
ITTTTTVTPT PTPTGTQTPT TTPITTTTTV TPTPTPTGTQ TPTTTPITTT
1960 1970 1980 1990 2000
TTVTPTPTPT GTQTPTTTPI TTTTTVTPTP TPTGTQTPTT TPITTTTTVT
2010 2020 2030 2040 2050
PTPTPTGTQT PTTTPITTTT TVTPTPTPTG TQTPTTTPIT TTTTVTPTPT
2060 2070 2080 2090 2100
PTGTQTPTTT PITTTTTVTP TPTPTGTQTP TTTPITTTTT VTPTPTPTGT
2110 2120 2130 2140 2150
QTPTTTPITT TTTVTPTPTP TGTQTPTTTP ITTTTTVTPT PTPTGTQTPT
2160 2170 2180 2190 2200
TTPITTTTTV TPTPTPTGTQ TPTTTPITTT TTVTPTPTPT GTQTPTTTPI
2210 2220 2230 2240 2250
TTTTTVTPTP TPTGTQTPTT TPITTTTTVT PTPTPTGTQT PTTTPITTTT
2260 2270 2280 2290 2300
TVTPTPTPTG TQTPTTTPIT TTTTVTPTPT PTGTQTPTTT PITTTTTVTP
2310 2320 2330 2340 2350
TPTPTGTQTP TTTPITTTTT VTPTPTPTGT QTPTTTPITT TTTVTPTPTP
2360 2370 2380 2390 2400
TGTQTPTTTP ITTTTTVTPT PTPTGTQTPT TTPITTTTTV TPTPTPTGTQ
2410 2420 2430 2440 2450
TPTTTPITTT TTVTPTPTPT GTQTPTTTPI TTTTTVTPTP TPTGTQTPTT
2460 2470 2480 2490 2500
TPITTTTTVT PTPTPTGTQT PTTTPITTTT TVTPTPTPTG TQTPTTTPIT
2510 2520 2530 2540 2550
TTTTVTPTPT PTGTQTPTTT PITTTTTVTP TPTPTGTQTP TTTPITTTTT
2560 2570 2580 2590 2600
VTPTPTPTGT QTPTTTPITT TTTVTPTPTP TGTQTPTTTP ITTTTTVTPT
2610 2620 2630 2640 2650
PTPTGTQTPT TTPITTTTTV TPTPTPTGTQ TPTTTPITTT TTVTPTPTPT
2660 2670 2680 2690 2700
GTQTPTTTPI TTTTTVTPTP TPTGTQTPTT TPITTTTTVT PTPTPTGTQT
2710 2720 2730 2740 2750
PTTTPITTTT TVTPTPTPTG TQTPTTTPIT TTTTVTPTPT PTGTQTPTTT
2760 2770 2780 2790 2800
PITTTTTVTP TPTPTGTQTP TTTPITTTTT VTPTPTPTGT QTPTTTPITT
2810 2820 2830 2840 2850
TTTVTPTPTP TGTQTPTTTP ITTTTTVTPT PTPTGTQTPT TTPITTTTTV
2860 2870 2880 2890 2900
TPTPTPTGTQ TPTTTPITTT TTVTPTPTPT GTQTPTTTPI TTTTTVTPTP
2910 2920 2930 2940 2950
TPTGTQTPTT TPITTTTTVT PTPTPTGTQT PTTTPITTTT TVTPTPTPTG
2960 2970 2980 2990 3000
TQTPTTTPIT TTTTVTPTPT PTGTQTPTTT PITTTTTVTP TPTPTGTQTP
3010 3020 3030 3040 3050
TTTPITTTTT VTPTPTPTGT QTPTTTPITT TTTVTPTPTP TGTQTPTTTP
3060 3070 3080 3090 3100
ITTTTTVTPT PTPTGTQTPT TTPITTTTTV TPTPTPTGTQ TPTTTPITTT
3110 3120 3130 3140 3150
TTVTPTPTPT GTQTPTTTPI TTTTTVTPTP TPTGTQTPTT TPITTTTTVT
3160 3170 3180 3190 3200
PTPTPTGTQT PTTTPITTTT TVTPTPTPTG TQTPTTTPIT TTTTVTPTPT
3210 3220 3230 3240 3250
PTGTQTPTTT PITTTTTVTP TPTPTGTQTP TTTPITTTTT VTPTPTPTGT
3260 3270 3280 3290 3300
QTPTTTPITT TTTVTPTPTP TGTQTPTTTP ITTTTTVTPT PTPTGTQTPT
3310 3320 3330 3340 3350
TTPITTTTTV TPTPTPTGTQ TPTTTPITTT TTVTPTPTPT GTQTPTTTPI
3360 3370 3380 3390 3400
TTTTTVTPTP TPTGTQTPTT TPITTTTTVT PTPTPTGTQT PTTTPITTTT
3410 3420 3430 3440 3450
TVTPTPTPTG TQTPTTTPIT TTTTVTPTPT PTGTQTPTTT PITTTTTVTP
3460 3470 3480 3490 3500
TPTPTGTQTP TTTPITTTTT VTPTPTPTGT QTPTTTPITT TTTVTPTPTP
3510 3520 3530 3540 3550
TGTQTPTTTP ITTTTTVTPT PTPTGTQTPT TTPITTTTTV TPTPTPTGTQ
3560 3570 3580 3590 3600
TPTTTPITTT TTVTPTPTPT GTQTPTTTPI TTTTTVTPTP TPTGTQTPTT
3610 3620 3630 3640 3650
TPITTTTTVT PTPTPTGTQT PTTTPITTTT TVTPTPTPTG TQTPTTTPIT
3660 3670 3680 3690 3700
TTTTVTPTPT PTGTQTPTTT PITTTTTVTP TPTPTGTQTP TTTPITTTTT
3710 3720 3730 3740 3750
VTPTPTPTGT QTPTTTPITT TTTVTPTPTP TGTQTPTTTP ITTTTTVTPT
3760 3770 3780 3790 3800
PTPTGTQTPT TTPITTTTTV TPTPTPTGTQ TPTTTPITTT TTVTPTPTPT
3810 3820 3830 3840 3850
GTQTPTTTPI TTTTTVTPTP TPTGTQTPTT TPITTTTTVT PTPTPTGTQT
3860 3870 3880 3890 3900
PTTTPITTTT TVTPTPTPTG TQTPTTTPIT TTTTVTPTPT PTGTQTPTTT
3910 3920 3930 3940 3950
PITTTTTVTP TPTPTGTQTP TTTPITTTTT VTPTPTPTGT QTPTTTPITT
3960 3970 3980 3990 4000
TTTVTPTPTP TGTQTPTTTP ITTTTTVTPT PTPTGTQTPT TTPITTTTTV
4010 4020 4030 4040 4050
TPTPTPTGTQ TPTTTPITTT TTVTPTPTPT GTQTPTTTPI TTTTTVTPTP
4060 4070 4080 4090 4100
TPTGTQTPTT TPITTTTTVT PTPTPTGTQT PTTTPITTTT TVTPTPTPTG
4110 4120 4130 4140 4150
TQTPTTTPIT TTTTVTPTPT PTGTQTPTTT PITTTTTVTP TPTPTGTQTP
4160 4170 4180 4190 4200
TTTPITTTTT VTPTPTPTGT QTPTTTPITT TTTVTPTPTP TGTQTGPPTH
4210 4220 4230 4240 4250
TSTAPIAELT TSNPPPESST PQTSRSTSSP LTESTTLLST LPPAIEMTST
4260 4270 4280 4290 4300
APPSTPTAPT TTSGGHTLSP PPSTTTSPPG TPTRGTTTGS SSAPTPSTVQ
4310 4320 4330 4340 4350
TTTTSAWTPT PTPLSTPSII RTTGLRPYPS SVLICCVLND TYYAPGEEVY
4360 4370 4380 4390 4400
NGTYGDTCYF VNCSLSCTLE FYNWSCPSTP SPTPTPSKST PTPSKPSSTP
4410 4420 4430 4440 4450
SKPTPGTKPP ECPDFDPPRQ ENETWWLCDC FMATCKYNNT VEIVKVECEP
4460 4470 4480 4490 4500
PPMPTCSNGL QPVRVEDPDG CCWHWECDCY CTGWGDPHYV TFDGLYYSYQ
4510 4520 4530 4540 4550
GNCTYVLVEE ISPSVDNFGV YIDNYHCDPN DKVSCPRTLI VRHETQEVLI
4560 4570 4580 4590 4600
KTVHMMPMQV QVQVNRQAVA LPYKKYGLEV YQSGINYVVD IPELGVLVSY
4610 4620 4630 4640 4650
NGLSFSVRLP YHRFGNNTKG QCGTCTNTTS DDCILPSGEI VSNCEAAADQ
4660 4670 4680 4690 4700
WLVNDPSKPH CPHSSSTTKR PAVTVPGGGK TTPHKDCTPS PLCQLIKDSL
4710 4720 4730 4740 4750
FAQCHALVPP QHYYDACVFD SCFMPGSSLE CASLQAYAAL CAQQNICLDW
4760 4770 4780 4790 4800
RNHTHGACLV ECPSHREYQA CGPAEEPTCK SSSSQQNNTV LVEGCFCPEG
4810 4820 4830 4840 4850
TMNYAPGFDV CVKTCGCVGP DNVPREFGEH FEFDCKNCVC LEGGSGIICQ
4860 4870 4880 4890 4900
PKRCSQKPVT HCVEDGTYLA TEVNPADTCC NITVCKCNTS LCKEKPSVCP
4910 4920 4930 4940 4950
LGFEVKSKMV PGRCCPFYWC ESKGVCVHGN AEYQPGSPVY SSKCQDCVCT
4960 4970 4980 4990 5000
DKVDNNTLLN VIACTHVPCN TSCSPGFELM EAPGECCKKC EQTHCIIKRP
5010 5020 5030 5040 5050
DNQHVILKPG DFKSDPKNNC TFFSCVKIHN QLISSVSNIT CPNFDASICI
5060 5070 5080 5090 5100
PGSITFMPNG CCKTCTPRNE TRVPCSTVPV TTEVSYAGCT KTVLMNHCSG
5110 5120 5130 5140 5150
SCGTFVMYSA KAQALDHSCS CCKEEKTSQR EVVLSCPNGG SLTHTYTHIE
5160 5170 5179
SCQCQDTVCG LPTGTSRRAR RSPRHLGSG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1497 Collapse assay details

Data source: Panorama

Official Gene Symbol
MUC2
Peptide Modified Sequence
GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK
Modification Type
Deamidated (N), Carbamidomethyl (C)
Protein - Site of Modification
423, 435
Peptide - Site of Modification
4, 16
Peptide Start
420
Peptide End
440
CPTAC ID
CPTAC-1497
Peptide Molecular Mass
2,219.9641
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 3.4 2.6 4.1 4.3 3.1 3.8 5.5 4 5.6 15 15 15
y8 (1+) 6.8 3.5 6.7 7.3 4.1 7.1 10 5.4 9.8 15 15 15
b9 (1+) 6.2 5.2 3.8 5.5 5.7 4.4 8.3 7.7 5.8 15 15 15
sum 2.7 1.3 2.2 3.4 1.5 2.7 4.3 2 3.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments