MUC2, CPTAC-1497 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MUC2	SMUC

Sequence Length (AA)	Molecular Weight (Da)
5179	540300

Protein Name
Mucin-2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGLPLARLAA	VCLALSLAGG	SELQTEGRTR	YHGRNVCSTW	GNFHYKTFDG
60	70	80	90	100
DVFRFPGLCD	YNFASDCRGS	YKEFAVHLKR	GPGQAEAPAG	VESILLTIKD
110	120	130	140	150
DTIYLTRHLA	VLNGAVVSTP	HYSPGLLIEK	SDAYTKVYSR	AGLTLMWNRE
160	170	180	190	200
DALMLELDTK	FRNHTCGLCG	DYNGLQSYSE	FLSDGVLFSP	LEFGNMQKIN
210	220	230	240	250
QPDVVCEDPE	EEVAPASCSE	HRAECERLLT	AEAFADCQDL	VPLEPYLRAC
260	270	280	290	300
QQDRCRCPGG	DTCVCSTVAE	FSRQCSHAGG	RPGNWRTATL	CPKTCPGNLV
310	320	330	340	350
YLESGSPCMD	TCSHLEVSSL	CEEHRMDGCF	CPEGTVYDDI	GDSGCVPVSQ
360	370	380	390	400
CHCRLHGHLY	TPGQEITNDC	EQCVCNAGRW	VCKDLPCPGT	CALEGGSHIT
410	420	430	440	450
TFDGKTYTFH	GDCYYVLAKG	DHNDSYALLG	ELAPCGSTDK	QTCLKTVVLL
460	470	480	490	500
ADKKKNAVVF	KSDGSVLLNQ	LQVNLPHVTA	SFSVFRPSSY	HIMVSMAIGV
510	520	530	540	550
RLQVQLAPVM	QLFVTLDQAS	QGQVQGLCGN	FNGLEGDDFK	TASGLVEATG
560	570	580	590	600
AGFANTWKAQ	STCHDKLDWL	DDPCSLNIES	ANYAEHWCSL	LKKTETPFGR
610	620	630	640	650
CHSAVDPAEY	YKRCKYDTCN	CQNNEDCLCA	ALSSYARACT	AKGVMLWGWR
660	670	680	690	700
EHVCNKDVGS	CPNSQVFLYN	LTTCQQTCRS	LSEADSHCLE	GFAPVDGCGC
710	720	730	740	750
PDHTFLDEKG	RCVPLAKCSC	YHRGLYLEAG	DVVVRQEERC	VCRDGRLHCR
760	770	780	790	800
QIRLIGQSCT	APKIHMDCSN	LTALATSKPR	ALSCQTLAAG	YYHTECVSGC
810	820	830	840	850
VCPDGLMDDG	RGGCVVEKEC	PCVHNNDLYS	SGAKIKVDCN	TCTCKRGRWV
860	870	880	890	900
CTQAVCHGTC	SIYGSGHYIT	FDGKYYDFDG	HCSYVAVQDY	CGQNSSLGSF
910	920	930	940	950
SIITENVPCG	TTGVTCSKAI	KIFMGRTELK	LEDKHRVVIQ	RDEGHHVAYT
960	970	980	990	1000
TREVGQYLVV	ESSTGIIVIW	DKRTTVFIKL	APSYKGTVCG	LCGNFDHRSN
1010	1020	1030	1040	1050
NDFTTRDHMV	VSSELDFGNS	WKEAPTCPDV	STNPEPCSLN	PHRRSWAEKQ
1060	1070	1080	1090	1100
CSILKSSVFS	ICHSKVDPKP	FYEACVHDSC	SCDTGGDCEC	FCSAVASYAQ
1110	1120	1130	1140	1150
ECTKEGACVF	WRTPDLCPIF	CDYYNPPHEC	EWHYEPCGNR	SFETCRTING
1160	1170	1180	1190	1200
IHSNISVSYL	EGCYPRCPKD	RPIYEEDLKK	CVTADKCGCY	VEDTHYPPGA
1210	1220	1230	1240	1250
SVPTEETCKS	CVCTNSSQVV	CRPEEGKILN	QTQDGAFCYW	EICGPNGTVE
1260	1270	1280	1290	1300
KHFNICSITT	RPSTLTTFTT	ITLPTTPTSF	TTTTTTTTPT	SSTVLSTTPK
1310	1320	1330	1340	1350
LCCLWSDWIN	EDHPSSGSDD	GDREPFDGVC	GAPEDIECRS	VKDPHLSLEQ
1360	1370	1380	1390	1400
HGQKVQCDVS	VGFICKNEDQ	FGNGPFGLCY	DYKIRVNCCW	PMDKCITTPS
1410	1420	1430	1440	1450
PPTTTPSPPP	TTTTTLPPTT	TPSPPTTTTT	TPPPTTTPSP	PITTTTTPLP
1460	1470	1480	1490	1500
TTTPSPPIST	TTTPPPTTTP	SPPTTTPSPP	TTTPSPPTTT	TTTPPPTTTP
1510	1520	1530	1540	1550
SPPMTTPITP	PASTTTLPPT	TTPSPPTTTT	TTPPPTTTPS	PPTTTPITPP
1560	1570	1580	1590	1600
TSTTTLPPTT	TPSPPPTTTT	TPPPTTTPSP	PTTTTPSPPT	ITTTTPPPTT
1610	1620	1630	1640	1650
TPSPPTTTTT	TPPPTTTPSP	PTTTPITPPT	STTTLPPTTT	PSPPPTTTTT
1660	1670	1680	1690	1700
PPPTTTPSPP	TTTTPSPPIT	TTTTPPPTTT	PSSPITTTPS	PPTTTMTTPS
1710	1720	1730	1740	1750
PTTTPSSPIT	TTTTPSSTTT	PSPPPTTMTT	PSPTTTPSPP	TTTMTTLPPT
1760	1770	1780	1790	1800
TTSSPLTTTP	LPPSITPPTF	SPFSTTTPTT	PCVPLCNWTG	WLDSGKPNFH
1810	1820	1830	1840	1850
KPGGDTELIG	DVCGPGWAAN	ISCRATMYPD	VPIGQLGQTV	VCDVSVGLIC
1860	1870	1880	1890	1900
KNEDQKPGGV	IPMAFCLNYE	INVQCCECVT	QPTTMTTTTT	ENPTPPTTTP
1910	1920	1930	1940	1950
ITTTTTVTPT	PTPTGTQTPT	TTPITTTTTV	TPTPTPTGTQ	TPTTTPITTT
1960	1970	1980	1990	2000
TTVTPTPTPT	GTQTPTTTPI	TTTTTVTPTP	TPTGTQTPTT	TPITTTTTVT
2010	2020	2030	2040	2050
PTPTPTGTQT	PTTTPITTTT	TVTPTPTPTG	TQTPTTTPIT	TTTTVTPTPT
2060	2070	2080	2090	2100
PTGTQTPTTT	PITTTTTVTP	TPTPTGTQTP	TTTPITTTTT	VTPTPTPTGT
2110	2120	2130	2140	2150
QTPTTTPITT	TTTVTPTPTP	TGTQTPTTTP	ITTTTTVTPT	PTPTGTQTPT
2160	2170	2180	2190	2200
TTPITTTTTV	TPTPTPTGTQ	TPTTTPITTT	TTVTPTPTPT	GTQTPTTTPI
2210	2220	2230	2240	2250
TTTTTVTPTP	TPTGTQTPTT	TPITTTTTVT	PTPTPTGTQT	PTTTPITTTT
2260	2270	2280	2290	2300
TVTPTPTPTG	TQTPTTTPIT	TTTTVTPTPT	PTGTQTPTTT	PITTTTTVTP
2310	2320	2330	2340	2350
TPTPTGTQTP	TTTPITTTTT	VTPTPTPTGT	QTPTTTPITT	TTTVTPTPTP
2360	2370	2380	2390	2400
TGTQTPTTTP	ITTTTTVTPT	PTPTGTQTPT	TTPITTTTTV	TPTPTPTGTQ
2410	2420	2430	2440	2450
TPTTTPITTT	TTVTPTPTPT	GTQTPTTTPI	TTTTTVTPTP	TPTGTQTPTT
2460	2470	2480	2490	2500
TPITTTTTVT	PTPTPTGTQT	PTTTPITTTT	TVTPTPTPTG	TQTPTTTPIT
2510	2520	2530	2540	2550
TTTTVTPTPT	PTGTQTPTTT	PITTTTTVTP	TPTPTGTQTP	TTTPITTTTT
2560	2570	2580	2590	2600
VTPTPTPTGT	QTPTTTPITT	TTTVTPTPTP	TGTQTPTTTP	ITTTTTVTPT
2610	2620	2630	2640	2650
PTPTGTQTPT	TTPITTTTTV	TPTPTPTGTQ	TPTTTPITTT	TTVTPTPTPT
2660	2670	2680	2690	2700
GTQTPTTTPI	TTTTTVTPTP	TPTGTQTPTT	TPITTTTTVT	PTPTPTGTQT
2710	2720	2730	2740	2750
PTTTPITTTT	TVTPTPTPTG	TQTPTTTPIT	TTTTVTPTPT	PTGTQTPTTT
2760	2770	2780	2790	2800
PITTTTTVTP	TPTPTGTQTP	TTTPITTTTT	VTPTPTPTGT	QTPTTTPITT
2810	2820	2830	2840	2850
TTTVTPTPTP	TGTQTPTTTP	ITTTTTVTPT	PTPTGTQTPT	TTPITTTTTV
2860	2870	2880	2890	2900
TPTPTPTGTQ	TPTTTPITTT	TTVTPTPTPT	GTQTPTTTPI	TTTTTVTPTP
2910	2920	2930	2940	2950
TPTGTQTPTT	TPITTTTTVT	PTPTPTGTQT	PTTTPITTTT	TVTPTPTPTG
2960	2970	2980	2990	3000
TQTPTTTPIT	TTTTVTPTPT	PTGTQTPTTT	PITTTTTVTP	TPTPTGTQTP
3010	3020	3030	3040	3050
TTTPITTTTT	VTPTPTPTGT	QTPTTTPITT	TTTVTPTPTP	TGTQTPTTTP
3060	3070	3080	3090	3100
ITTTTTVTPT	PTPTGTQTPT	TTPITTTTTV	TPTPTPTGTQ	TPTTTPITTT
3110	3120	3130	3140	3150
TTVTPTPTPT	GTQTPTTTPI	TTTTTVTPTP	TPTGTQTPTT	TPITTTTTVT
3160	3170	3180	3190	3200
PTPTPTGTQT	PTTTPITTTT	TVTPTPTPTG	TQTPTTTPIT	TTTTVTPTPT
3210	3220	3230	3240	3250
PTGTQTPTTT	PITTTTTVTP	TPTPTGTQTP	TTTPITTTTT	VTPTPTPTGT
3260	3270	3280	3290	3300
QTPTTTPITT	TTTVTPTPTP	TGTQTPTTTP	ITTTTTVTPT	PTPTGTQTPT
3310	3320	3330	3340	3350
TTPITTTTTV	TPTPTPTGTQ	TPTTTPITTT	TTVTPTPTPT	GTQTPTTTPI
3360	3370	3380	3390	3400
TTTTTVTPTP	TPTGTQTPTT	TPITTTTTVT	PTPTPTGTQT	PTTTPITTTT
3410	3420	3430	3440	3450
TVTPTPTPTG	TQTPTTTPIT	TTTTVTPTPT	PTGTQTPTTT	PITTTTTVTP
3460	3470	3480	3490	3500
TPTPTGTQTP	TTTPITTTTT	VTPTPTPTGT	QTPTTTPITT	TTTVTPTPTP
3510	3520	3530	3540	3550
TGTQTPTTTP	ITTTTTVTPT	PTPTGTQTPT	TTPITTTTTV	TPTPTPTGTQ
3560	3570	3580	3590	3600
TPTTTPITTT	TTVTPTPTPT	GTQTPTTTPI	TTTTTVTPTP	TPTGTQTPTT
3610	3620	3630	3640	3650
TPITTTTTVT	PTPTPTGTQT	PTTTPITTTT	TVTPTPTPTG	TQTPTTTPIT
3660	3670	3680	3690	3700
TTTTVTPTPT	PTGTQTPTTT	PITTTTTVTP	TPTPTGTQTP	TTTPITTTTT
3710	3720	3730	3740	3750
VTPTPTPTGT	QTPTTTPITT	TTTVTPTPTP	TGTQTPTTTP	ITTTTTVTPT
3760	3770	3780	3790	3800
PTPTGTQTPT	TTPITTTTTV	TPTPTPTGTQ	TPTTTPITTT	TTVTPTPTPT
3810	3820	3830	3840	3850
GTQTPTTTPI	TTTTTVTPTP	TPTGTQTPTT	TPITTTTTVT	PTPTPTGTQT
3860	3870	3880	3890	3900
PTTTPITTTT	TVTPTPTPTG	TQTPTTTPIT	TTTTVTPTPT	PTGTQTPTTT
3910	3920	3930	3940	3950
PITTTTTVTP	TPTPTGTQTP	TTTPITTTTT	VTPTPTPTGT	QTPTTTPITT
3960	3970	3980	3990	4000
TTTVTPTPTP	TGTQTPTTTP	ITTTTTVTPT	PTPTGTQTPT	TTPITTTTTV
4010	4020	4030	4040	4050
TPTPTPTGTQ	TPTTTPITTT	TTVTPTPTPT	GTQTPTTTPI	TTTTTVTPTP
4060	4070	4080	4090	4100
TPTGTQTPTT	TPITTTTTVT	PTPTPTGTQT	PTTTPITTTT	TVTPTPTPTG
4110	4120	4130	4140	4150
TQTPTTTPIT	TTTTVTPTPT	PTGTQTPTTT	PITTTTTVTP	TPTPTGTQTP
4160	4170	4180	4190	4200
TTTPITTTTT	VTPTPTPTGT	QTPTTTPITT	TTTVTPTPTP	TGTQTGPPTH
4210	4220	4230	4240	4250
TSTAPIAELT	TSNPPPESST	PQTSRSTSSP	LTESTTLLST	LPPAIEMTST
4260	4270	4280	4290	4300
APPSTPTAPT	TTSGGHTLSP	PPSTTTSPPG	TPTRGTTTGS	SSAPTPSTVQ
4310	4320	4330	4340	4350
TTTTSAWTPT	PTPLSTPSII	RTTGLRPYPS	SVLICCVLND	TYYAPGEEVY
4360	4370	4380	4390	4400
NGTYGDTCYF	VNCSLSCTLE	FYNWSCPSTP	SPTPTPSKST	PTPSKPSSTP
4410	4420	4430	4440	4450
SKPTPGTKPP	ECPDFDPPRQ	ENETWWLCDC	FMATCKYNNT	VEIVKVECEP
4460	4470	4480	4490	4500
PPMPTCSNGL	QPVRVEDPDG	CCWHWECDCY	CTGWGDPHYV	TFDGLYYSYQ
4510	4520	4530	4540	4550
GNCTYVLVEE	ISPSVDNFGV	YIDNYHCDPN	DKVSCPRTLI	VRHETQEVLI
4560	4570	4580	4590	4600
KTVHMMPMQV	QVQVNRQAVA	LPYKKYGLEV	YQSGINYVVD	IPELGVLVSY
4610	4620	4630	4640	4650
NGLSFSVRLP	YHRFGNNTKG	QCGTCTNTTS	DDCILPSGEI	VSNCEAAADQ
4660	4670	4680	4690	4700
WLVNDPSKPH	CPHSSSTTKR	PAVTVPGGGK	TTPHKDCTPS	PLCQLIKDSL
4710	4720	4730	4740	4750
FAQCHALVPP	QHYYDACVFD	SCFMPGSSLE	CASLQAYAAL	CAQQNICLDW
4760	4770	4780	4790	4800
RNHTHGACLV	ECPSHREYQA	CGPAEEPTCK	SSSSQQNNTV	LVEGCFCPEG
4810	4820	4830	4840	4850
TMNYAPGFDV	CVKTCGCVGP	DNVPREFGEH	FEFDCKNCVC	LEGGSGIICQ
4860	4870	4880	4890	4900
PKRCSQKPVT	HCVEDGTYLA	TEVNPADTCC	NITVCKCNTS	LCKEKPSVCP
4910	4920	4930	4940	4950
LGFEVKSKMV	PGRCCPFYWC	ESKGVCVHGN	AEYQPGSPVY	SSKCQDCVCT
4960	4970	4980	4990	5000
DKVDNNTLLN	VIACTHVPCN	TSCSPGFELM	EAPGECCKKC	EQTHCIIKRP
5010	5020	5030	5040	5050
DNQHVILKPG	DFKSDPKNNC	TFFSCVKIHN	QLISSVSNIT	CPNFDASICI
5060	5070	5080	5090	5100
PGSITFMPNG	CCKTCTPRNE	TRVPCSTVPV	TTEVSYAGCT	KTVLMNHCSG
5110	5120	5130	5140	5150
SCGTFVMYSA	KAQALDHSCS	CCKEEKTSQR	EVVLSCPNGG	SLTHTYTHIE
5160	5170	5179
SCQCQDTVCG	LPTGTSRRAR	RSPRHLGSG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1497 Collapse assay details

Data source: Panorama

Official Gene Symbol: MUC2
Peptide Modified Sequence: GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK
Modification Type: Deamidated (N), Carbamidomethyl (C)
Protein - Site of Modification: 423, 435
Peptide - Site of Modification: 4, 16
Peptide Start: 420
Peptide End: 440

CPTAC ID: CPTAC-1497
Peptide Molecular Mass: 2,219.9641
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

CPTAC-1479: F3. N[+1.0]NTFLSLR	CPTAC-1480: CADM1. TDN[+1.0]GTYR	CPTAC-1481: CD164. TVTTSGTTN[+1.0]NTVTPTSQPVR
CPTAC-1483: CHST3. N[+1.0]SSEQFEK	CPTAC-1484: CNDP1. LVPHM[+16.0]N[+1.0]VSAVEK	CPTAC-1485: COL6A3. QEVLSHISN[+1.0]M[+16.0]SYIGGTNQTGK
CPTAC-1486: CPE. GN[+1.0]ETIVNLIHSTR	CPTAC-1488: DPY19L4. GVYELTHNN[+1.0]K	CPTAC-1489: ENTPD2. LLN[+1.0]LTNPEASTSVLM[+16.0]AVTHTLTQYPFDFR
CPTAC-1490: BPIFB1. DHN[+1.0]ATSILQQLPLLSAM[+16.0]R	CPTAC-1491: FOLH1. VPYNVGPGFTGN[+1.0]FSTQK	CPTAC-1492: ITGA9. N[+1.0]QTVFER
CPTAC-1493: GGT7. TAN[+1.0]HSAPSLENSVQPGK	CPTAC-1494: LAMP2. VASVININPN[+1.0]TTHSTGSC[+57.0]R	CPTAC-1495: LRIG1. LHELVLSFNN[+1.0]LTR
CPTAC-1496: MET. FINFFVGNTIN[+1.0]SSYFPDHPLHSISVR	CPTAC-1497: MUC2. GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK	CPTAC-1498: MUC5B. YSFEGSC[+57.0]EYILAQDYC[+57.0]GDN[+1.0]TTHGTFR
CPTAC-1499: PIGT. TYAIYDLLDTAM[+16.0]IN[+1.0]NSR	CPTAC-1500: PLOD1. EQIN[+1.0]ITLDHR	CPTAC-1501: PLOD3. EQYIHEN[+1.0]YSR
CPTAC-1503: ALCAM. TIIC[+57.0]HVEGFPKPAIQWTITGSGSVIN[+1.0]QTEESPYINGR	CPTAC-1504: PLXNB2. LSHDAN[+1.0]ETLPLHLYVK	CPTAC-1506: POFUT1. EGNPFGPFWDQFHVSFN[+1.0]K
CPTAC-1507: SCGB2A1. QC[+57.0]FLN[+1.0]QSHR	CPTAC-1508: SERPIND1. DFVN[+1.0]ASSK	CPTAC-1509: SUN1. N[+1.0]VTHHVSVTK
CPTAC-1510: TACSTD2. HRPTAGAFN[+1.0]HSDLDAELR	CPTAC-1511: THY1. DEGTYTC[+57.0]ALHHSGHSPPISSQN[+1.0]VTVLR	CPTAC-1512: TMX4. VQPM[+16.0]TASN[+1.0]WTLVM[+16.0]EGEWM[+16.0]LK
CPTAC-1513: TWSG1. N[+1.0]YSDTPPTSK	CPTAC-1514: PPT1. FLN[+1.0]DSIVDPVDSEWFGFYR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	3.4	2.6	4.1	4.3	3.1	3.8	5.5	4	5.6	15	15	15
y8 (1+)	6.8	3.5	6.7	7.3	4.1	7.1	10	5.4	9.8	15	15	15
b9 (1+)	6.2	5.2	3.8	5.5	5.7	4.4	8.3	7.7	5.8	15	15	15
sum	2.7	1.3	2.2	3.4	1.5	2.7	4.3	2	3.5	15	15	15

MUC2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1497 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

MUC2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1497 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments