Alcam, CPTAC-3640 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Alcam	N/A

Sequence Length (AA)	Molecular Weight (Da)
583	65092

Protein Name
CD166 antigen

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MASKVSPSCR	LVFCLLISAA	VLRPGLGWYT	VNSAYGDTIV	MPCRLDVPQN
60	70	80	90	100
LMFGKWKYEK	PDGSPVFIAF	RSSTKKSVQY	DDVPEYKDRL	SLSENYTLSI
110	120	130	140	150
ANAKISDEKR	FVCMLVTEDN	VFEAPTLVKV	FKQPSKPEIV	NKAPFLETDQ
160	170	180	190	200
LKKLGDCISR	DSYPDGNITW	YRNGKVLQPV	EGEVAILFKK	EIDPGTQLYT
210	220	230	240	250
VTSSLEYKTT	RSDIQMPFTC	SVTYYGPSGQ	KTIYSEQEIF	DIYYPTEQVT
260	270	280	290	300
IQVLPPKNAI	KEGDNITLQC	LGNGNPPPEE	FMFYLPGQPE	GIRSSNTYTL
310	320	330	340	350
TDVRRNATGD	YKCSLIDKRN	MAASTTITVH	YLDLSLNPSG	EVTKQIGDTL
360	370	380	390	400
PVSCTISASR	NATVVWMKDN	IRLRSSPSFS	SLHYQDAGNY	VCETALQEVE
410	420	430	440	450
GLKKRESLTL	IVEGKPQIKM	TKKTDPSGLS	KTIICHVEGF	PKPAIHWTIT
460	470	480	490	500
GSGSVINQTE	ESPYINGRYY	SKIIISPEEN	VTLTCTAENQ	LERTVNSLNV
510	520	530	540	550
SAISIPEHDE	ADDISDENRE	KVNDQAKLIV	GIVVGLLLAA	LVAGVVYWLY
560	570	580	583
MKKSKTASKH	VNKDLGNMEE	NKKLEENNHK	TEA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-6040 Collapse assay details

Data source: Panorama

Official Gene Symbol: ALCAM
Peptide Sequence: ALFLETEQLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 143
Peptide End: 152

CPTAC ID: CPTAC-6040
Peptide Molecular Mass: 1,190.6547
Species: Human
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: FFPE lung tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.075 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	13.4	5.6	6.8	16.8	8.3	7.2	21.5	10	9.9	15	15	15
y4 (1+)	22.7	8	8.5	23.5	7.1	8.6	32.7	10.7	12.1	15	15	15
y5 (1+)	17.9	5.6	6.4	20.8	7.9	8.9	27.4	9.7	11	15	15	15
y6 (1+)	14.5	6.1	6.3	16.4	5.7	6.6	21.9	8.3	9.1	15	15	15
y7 (1+)	17.3	5.6	6.5	20.3	6.2	8.5	26.7	8.4	10.7	15	15	15
y8 (1+)	13.8	5.8	5.6	17.1	6.1	9.9	22	8.4	11.4	15	15	15
y8 (2+)	12.6	6.9	8.1	19.6	5	6.6	23.3	8.5	10.4	15	15	15
sum	13.7	5.8	6.1	17	6.1	7.4	21.8	8.4	9.6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1502 Collapse assay details

Data source: Panorama

Official Gene Symbol: ALCAM
Peptide Modified Sequence: LN[+1.0]LSEN[+1.0]YTLSISNAR
Modification Type: Deamidated (N), Deamidated (N)
Protein - Site of Modification: 91, 95
Peptide - Site of Modification: 2, 6
Peptide Start: 90
Peptide End: 104

CPTAC ID: CPTAC-1502
Peptide Molecular Mass: 1,695.8315
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang