Chil3, CPTAC-3641 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Chil3	Chi3l3, Ym1

Sequence Length (AA)	Molecular Weight (Da)
398	44458

Protein Name
Chitinase-like protein 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAKLILVTGL	AILLNVQLGS	SYQLMCYYTS	WAKDRPIEGS	FKPGNIDPCL
60	70	80	90	100
CTHLIYAFAG	MQNNEITYTH	EQDLRDYEAL	NGLKDKNTEL	KTLLAIGGWK
110	120	130	140	150
FGPAPFSAMV	STPQNRQIFI	QSVIRFLRQY	NFDGLNLDWQ	YPGSRGSPPK
160	170	180	190	200
DKHLFSVLVK	EMRKAFEEES	VEKDIPRLLL	TSTGAGIIDV	IKSGYKIPEL
210	220	230	240	250
SQSLDYIQVM	TYDLHDPKDG	YTGENSPLYK	SPYDIGKSAD	LNVDSIISYW
260	270	280	290	300
KDHGAASEKL	IVGFPAYGHT	FILSDPSKTG	IGAPTISTGP	PGKYTDESGL
310	320	330	340	350
LAYYEVCTFL	NEGATEVWDA	PQEVPYAYQG	NEWVGYDNVR	SFKLKAQWLK
360	370	380	390	398
DNNLGGAVVW	PLDMDDFSGS	FCHQRHFPLT	STLKGDLNIH	SASCKGPY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3641 Collapse assay details

Data source: Panorama

Official Gene Symbol: Chil3
Peptide Sequence: AFEEESVEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 165
Peptide End: 173

CPTAC ID: non-CPTAC-3641
Peptide Molecular Mass: 1,066.4819
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	7.5	3.2	5.6	10	6.9	5.3	12.5	7.6	7.7	15	15	15
y8 (2+)	25.4	11	9.3	24.7	10.2	11.4	35.4	15	14.7	15	15	15
y2 (1+)	35	10.8	8.4	30	9.2	9.7	46.1	14.2	12.8	15	15	15
y6 (1+)	17.3	5.7	4.7	18.9	5.8	7	25.6	8.1	8.4	15	15	15
y7 (1+)	6.4	3.4	2.7	10.6	6.2	3.7	12.4	7.1	4.6	15	15	15
sum	3.6	2.1	2.7	8.6	4.6	3.9	9.3	5.1	4.7	15	15	15

Chil3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3641 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Chil3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3641 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments