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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Cntn1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Cntn1 N/A
Sequence Length (AA) Molecular Weight (Da)
1020 113388
Protein Name
Contactin-1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKMPLLVSHL LLISLTSCLG DFTWHRRYGH GVSEEDKGFG PIFEEQPINT
60 70 80 90 100
IYPEESLEGK VSLNCRARAS PFPVYKWRMN NGDVDLTNDR YSMVGGNLVI
110 120 130 140 150
NNPDKQKDAG VYYCLASNNY GMVRSTEATL SFGYLDPFPP EERPEVKVKE
160 170 180 190 200
GKGMVLLCDP PYHFPDDLSY RWLLNEFPVF ITMDKRRFVS QTNGNLYIAN
210 220 230 240 250
VESSDRGNYS CFVSSPSITK SVFSKFIPLI PIPERTTKPY PADIVVQFKD
260 270 280 290 300
IYTMMGQNVT LECFALGNPV PDIRWRKVLE PMPSTAEIST SGAVLKIFNI
310 320 330 340 350
QLEDEGLYEC EAENIRGKDK HQARIYVQAF PEWVEHINDT EVDIGSDLYW
360 370 380 390 400
PCIATGKPIP TIRWLKNGYS YHKGELRLYD VTFENAGMYQ CIAENAYGSI
410 420 430 440 450
YANAELKILA LAPTFEMNPM KKKILAAKGG RVIIECKPKA APKPKFSWSK
460 470 480 490 500
GTEWLVNSSR ILIWEDGSLE INNITRNDGG IYTCFAENNR GKANSTGTLV
510 520 530 540 550
ITNPTRIILA PINADITVGE NATMQCAASF DPALDLTFVW SFNGYVIDFN
560 570 580 590 600
KEITHIHYQR NFMLDANGEL LIRNAQLKHA GRYTCTAQTI VDNSSASADL
610 620 630 640 650
VVRGPPGPPG GLRIEDIRAT SVALTWSRGS DNHSPISKYT IQTKTILSDD
660 670 680 690 700
WKDAKTDPPI IEGNMESAKA VDLIPWMEYE FRVVATNTLG TGEPSIPSNR
710 720 730 740 750
IKTDGAAPNV APSDVGGGGG TNRELTITWA PLSREYHYGN NFGYIVAFKP
760 770 780 790 800
FDGEEWKKVT VTNPDTGRYV HKDETMTPST AFQVKVKAFN NKGDGPYSLV
810 820 830 840 850
AVINSAQDAP SEAPTEVGVK VLSSSEISVH WKHVLEKIVE SYQIRYWAGH
860 870 880 890 900
DKEAAAHRVQ VTSQEYSARL ENLLPDTQYF IEVGACNSAG CGPSSDVIET
910 920 930 940 950
FTRKAPPSQP PRIISSVRSG SRYIITWDHV VALSNESTVT GYKILYRPDG
960 970 980 990 1000
QHDGKLFSTH KHSIEVPIPR DGEYVVEVRA HSDGGDGVVS QVKISGVSTL
1010 1020
SSSLLSLLLP SLGFLVYSEF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3642 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cntn1
Peptide Sequence
EITHIHYQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
552
Peptide End
560
CPTAC ID
non-CPTAC-3642
Peptide Molecular Mass
1,195.6098
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (3+) 7.4 3.5 5.2 11.2 6.3 5.5 13.4 7.2 7.6 15 15 15
y6 (2+) 5.2 2 2.3 9.1 4.9 3.1 10.5 5.3 3.9 15 15 15
y7 (2+) 4.9 2.6 2.4 8 4.2 4.8 9.4 4.9 5.4 15 15 15
y3 (1+) 4.7 2.3 2.6 11.6 4.2 4.2 12.5 4.8 4.9 15 15 15
y4 (1+) 4.7 2.6 3.3 12.2 3.8 5.6 13.1 4.6 6.5 15 15 15
sum 4.1 1.8 1.3 8.5 4.1 3.6 9.4 4.5 3.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments