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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Fah N/A
Sequence Length (AA) Molecular Weight (Da)
419 46176
Protein Name
Fumarylacetoacetase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSFIPVAEDS DFPIQNLPYG VFSTQSNPKP RIGVAIGDQI LDLSVIKHLF
60 70 80 90 100
TGPALSKHQH VFDETTLNNF MGLGQAAWKE ARASLQNLLS ASQARLRDDK
110 120 130 140 150
ELRQRAFTSQ ASATMHLPAT IGDYTDFYSS RQHATNVGIM FRGKENALLP
160 170 180 190 200
NWLHLPVGYH GRASSIVVSG TPIRRPMGQM RPDNSKPPVY GACRLLDMEL
210 220 230 240 250
EMAFFVGPGN RFGEPIPISK AHEHIFGMVL MNDWSARDIQ QWEYVPLGPF
260 270 280 290 300
LGKSFGTTIS PWVVPMDALM PFVVPNPKQD PKPLPYLCHS QPYTFDINLS
310 320 330 340 350
VSLKGEGMSQ AATICRSNFK HMYWTMLQQL THHSVNGCNL RPGDLLASGT
360 370 380 390 400
ISGSDPESFG SMLELSWKGT KAIDVEQGQT RTFLLDGDEV IITGHCQGDG
410 419
YRVGFGQCAG KVLPALSPA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3803 Collapse assay details

Data source: Panorama

Official Gene Symbol
Fah
Peptide Sequence
ASSIVVSGTPIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
163
Peptide End
174
CPTAC ID
non-CPTAC-3803
Peptide Molecular Mass
1,185.6717
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 16.3 8.3 6.9 16.9 8.6 9 23.5 12 11.3 15 15 15
y7 (1+) 12.2 8 6.5 12.3 8.2 12.3 17.3 11.5 13.9 15 15 15
y6 (1+) 12.9 7.1 8.9 19.2 7.6 10.2 23.1 10.4 13.5 15 15 15
y5 (1+) 15.4 5.5 8.2 14.6 9.4 10 21.2 10.9 12.9 15 15 15
b5 (1+) 18.4 10.3 8 21.5 10.6 11.7 28.3 14.8 14.2 15 15 15
sum 7.6 5.3 3.2 7.3 6.8 6.3 10.5 8.6 7.1 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3643 Collapse assay details

Data source: Panorama

Official Gene Symbol
Fah
Peptide Sequence
FGEPIPISK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
212
Peptide End
220
CPTAC ID
non-CPTAC-3643
Peptide Molecular Mass
986.5437
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 12 14.3 11.9 18 20 23.1 21.6 24.6 26 15 15 15
y6 (1+) 8.9 6 8.6 17.2 9.8 9.2 19.4 11.5 12.6 15 15 15
b3 (1+) 18.6 18.2 20.9 20.1 22 28.3 27.4 28.6 35.2 15 15 15
b2 (1+) 10.5 19 21 12.2 12.7 21.7 16.1 22.9 30.2 15 15 15
y2 (1+) 15.8 9.9 13.2 23.2 21.5 14.7 28.1 23.7 19.8 15 15 15
sum 10.4 5.3 8.3 14.4 10.7 9 17.8 11.9 12.2 15 15 15

Additional Resources and Comments