Fah, CPTAC-3803 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Fah	N/A

Sequence Length (AA)	Molecular Weight (Da)
419	46176

Protein Name
Fumarylacetoacetase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSFIPVAEDS	DFPIQNLPYG	VFSTQSNPKP	RIGVAIGDQI	LDLSVIKHLF
60	70	80	90	100
TGPALSKHQH	VFDETTLNNF	MGLGQAAWKE	ARASLQNLLS	ASQARLRDDK
110	120	130	140	150
ELRQRAFTSQ	ASATMHLPAT	IGDYTDFYSS	RQHATNVGIM	FRGKENALLP
160	170	180	190	200
NWLHLPVGYH	GRASSIVVSG	TPIRRPMGQM	RPDNSKPPVY	GACRLLDMEL
210	220	230	240	250
EMAFFVGPGN	RFGEPIPISK	AHEHIFGMVL	MNDWSARDIQ	QWEYVPLGPF
260	270	280	290	300
LGKSFGTTIS	PWVVPMDALM	PFVVPNPKQD	PKPLPYLCHS	QPYTFDINLS
310	320	330	340	350
VSLKGEGMSQ	AATICRSNFK	HMYWTMLQQL	THHSVNGCNL	RPGDLLASGT
360	370	380	390	400
ISGSDPESFG	SMLELSWKGT	KAIDVEQGQT	RTFLLDGDEV	IITGHCQGDG
410	419
YRVGFGQCAG	KVLPALSPA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3803 Collapse assay details

Data source: Panorama

Official Gene Symbol: Fah
Peptide Sequence: ASSIVVSGTPIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 163
Peptide End: 174

CPTAC ID: non-CPTAC-3803
Peptide Molecular Mass: 1,185.6717
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	16.3	8.3	6.9	16.9	8.6	9	23.5	12	11.3	15	15	15
y7 (1+)	12.2	8	6.5	12.3	8.2	12.3	17.3	11.5	13.9	15	15	15
y6 (1+)	12.9	7.1	8.9	19.2	7.6	10.2	23.1	10.4	13.5	15	15	15
y5 (1+)	15.4	5.5	8.2	14.6	9.4	10	21.2	10.9	12.9	15	15	15
b5 (1+)	18.4	10.3	8	21.5	10.6	11.7	28.3	14.8	14.2	15	15	15
sum	7.6	5.3	3.2	7.3	6.8	6.3	10.5	8.6	7.1	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3643 Collapse assay details

Data source: Panorama

Official Gene Symbol: Fah
Peptide Sequence: FGEPIPISK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 212
Peptide End: 220

CPTAC ID: non-CPTAC-3643
Peptide Molecular Mass: 986.5437
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers