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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Erap1 Appils, Arts1
Sequence Length (AA) Molecular Weight (Da)
930 106599
Protein Name
Endoplasmic reticulum aminopeptidase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPSLLPLVLT FLSVSSPSWC QNSDIESLKA SNGDSFPWNN MRLPEYMTPI
60 70 80 90 100
HYDLMIHANL STLTFWGKTE VEIIASRPTS TIIMHSHHLQ ISKATLRRGA
110 120 130 140 150
GEMLSEEPLK VLEYPAHEQV ALLAAQPLLA GSLYTVIIDY AANLSESFHG
160 170 180 190 200
FYKSTYRTQE GEMRILAATQ FEPTAARMAF PCFDEPALKA SFSIKIKRDP
210 220 230 240 250
RHLAISNMPL VKSVNVAEGL IEDHFDITVK MSTYLVAFII SDFKSVSKMT
260 270 280 290 300
KSGVKVSVYA VPDKINQADY ALDAAVTLLE FYEDYFNIPY PLPKQDLAAI
310 320 330 340 350
PDFQSGAMEN WGLTTYRESS LLYDKEKSSA SSKLGITMIV SHELAHQWFG
360 370 380 390 400
NLVTMEWWND LWLNEGFAKF MEFVSVTVTH PELKVEDYFF GKCFNAMEVD
410 420 430 440 450
ALNSSHPVST PVENPAQIRE MFDDVSYEKG ACILNMLRDY LSADTFKRGI
460 470 480 490 500
VQYLQKYSYK NTKNEDLWNS MMHICPTDGT QTMDGFCSRS QHSSSTSHWR
510 520 530 540 550
QEVVDVKTMM NTWTLQKGFP LITITVSGRN VHMKQEHYMK GSERFPETGY
560 570 580 590 600
LWHVPLTFIT SKSDSVQRFL LKTKTDVLIL PEAVQWIKFN VGMNGYYIVH
610 620 630 640 650
YADDGWASLS GLLKEAHTTI SSNDRASLIN NAFQLVSIEK LSIEKALDLT
660 670 680 690 700
LYLKNETEIM PIFQALNELI PMYKLMEKRD MIEVETQFKD FLLKLLKDLI
710 720 730 740 750
DKQTWTDEGS VSERMLRSQL LLLACVRNYQ PCVQRAERYF REWKSSNGNM
760 770 780 790 800
SIPIDVTLAV FAVGAQNTEG WDFLYSKYQS SLSSTEKSQI EFSLCTSKDP
810 820 830 840 850
EKLQWLLDQS FKGEIIKTQE FPHILTLIGR NPVGYPLAWK FLRENWNKLV
860 870 880 890 900
QKFELGSSSI AHMVMGTTDQ FSTRARLEEV KGFFSSLKEN GSQLRCVQQT
910 920 930
IETIEENIRW MDKNFDKIRL WLQKEKPELL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3804 Collapse assay details

Data source: Panorama

Official Gene Symbol
Erap1
Peptide Sequence
GIVQYLQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
449
Peptide End
456
CPTAC ID
non-CPTAC-3804
Peptide Molecular Mass
947.5440
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 13.3 3.9 6.7 17.2 12.8 12.2 21.7 13.4 13.9 15 15 15
y6 (2+) 4.9 4.5 2.2 6.8 7.1 3.9 8.4 8.4 4.5 15 15 15
y4 (1+) 5.1 5.4 4.5 10.2 9.6 5.4 11.4 11 7 15 15 15
y5 (1+) 5.2 4.6 5.1 9.7 7.8 6.2 11 9.1 8 15 15 15
y6 (1+) 2.9 4 4 6.9 8.2 5.2 7.5 9.1 6.6 15 15 15
sum 2.6 1.7 1.5 7 7.4 3.5 7.5 7.6 3.8 15 15 15


Additional Resources and Comments