Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Efemp1 Fbln3
Sequence Length (AA) Molecular Weight (Da)
493 54953
Protein Name
EGF-containing fibulin-like extracellular matrix protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLQTLFLTML TLALVKSQYT EETITYTQCT DGYEWDPIRQ QCKDIDECDI
60 70 80 90 100
VPDACKGGMK CVNHYGGYLC LPKTAQIIVN NEHPQQETPA AEASSGATTG
110 120 130 140 150
TVAARSMATS GVVPGGGFMA SATAVAGPEV QTGRNNFVIR RNPADPQRIP
160 170 180 190 200
SNPSHRIQCA AGYEQSEHNV CQDIDECTSG THNCRTDQVC INLRGSFTCQ
210 220 230 240 250
CLPGYQKRGE QCVDIDECTV PPYCHQRCVN TPGSFYCQCS PGFQLAANNY
260 270 280 290 300
TCVDINECDA SNQCAQQCYN ILGSFICQCN QGYELSSDRL NCEDIDECRT
310 320 330 340 350
SSYLCQYQCV NEPGKFSCMC PQGYEVVRSR TCQDINECET TNECREDEMC
360 370 380 390 400
WNYHGGFRCY PRNPCQDHYV LTSENRCVCP VSNTMCRELP QSIVYKYMSI
410 420 430 440 450
RSDRSVPSDI FQIQATMIYA NTINTFRIKS GNENGEFYLR QTSPVSAMLV
460 470 480 490 493
LVKSLSGPRE YIVDLEMLTV SSIGTFRTSS VLRLTIIVGP FSF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-5606 Collapse assay details

Data source: Panorama

Official Gene Symbol
Efemp1
Peptide Sequence
ELPQSIVYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
388
Peptide End
396
CPTAC ID
non-CPTAC-5606
Peptide Molecular Mass
1,075.5914
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 28.2 6.3 2.4 60.8 12.7 4.9 67 14.2 5.5 15 15 15
y7 (2+) 2.8 1.6 1.5 5.7 6.4 3.6 6.4 6.6 3.9 15 15 15
y2 (1+) 23.6 6.6 8.6 30.2 10.2 9.1 38.3 12.1 12.5 15 15 15
y5 (1+) 11.1 3.2 4.5 12.7 6.8 5.1 16.9 7.5 6.8 15 15 15
y7 (1+) 8.5 1.8 1.1 8.5 6.9 2.6 12 7.1 2.8 15 15 15
sum 6.3 1.3 1.1 13.7 6.2 3.2 15.1 6.3 3.4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3805 Collapse assay details

Data source: Panorama

Official Gene Symbol
Efemp1
Peptide Sequence
SGNENGEFYLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
430
Peptide End
440
CPTAC ID
non-CPTAC-3805
Peptide Molecular Mass
1,284.5735
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 7.8 5.1 3.8 8.3 7.5 6.2 11.4 9.1 7.3 15 15 15
y3 (1+) 18.1 4.7 6.2 17.7 8.9 7.8 25.3 10.1 10 15 15 15
y4 (1+) 12.6 3.4 4.3 15.5 4.1 7 20 5.3 8.2 15 15 15
y6 (1+) 10.4 7.4 4.6 13 9.3 5.5 16.6 11.9 7.2 15 15 15
y7 (1+) 10.1 4.1 5.4 9.9 8.3 5.9 14.1 9.3 8 15 15 15
sum 5.7 1.7 2.7 7 4.9 3.8 9 5.2 4.7 15 15 15


Additional Resources and Comments