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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CRP PTX1
Sequence Length (AA) Molecular Weight (Da)
224 25039
Protein Name
C-reactive protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEKLLCFLVL TSLSHAFGQT DMSRKAFVFP KESDTSYVSL KAPLTKPLKA
60 70 80 90 100
FTVCLHFYTE LSSTRGYSIF SYATKRQDNE ILIFWSKDIG YSFTVGGSEI
110 120 130 140 150
LFEVPEVTVA PVHICTSWES ASGIVEFWVD GKPRVRKSLK KGYTVGAEAS
160 170 180 190 200
IILGQEQDSF GGNFEGSQSL VGDIGNVNMW DFVLSPDEIN TIYLGGPFSP
210 220 224
NVLNWRALKY EVQGEVFTKP QLWP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1478 Collapse assay details

Data source: Panorama

Official Gene Symbol
CRP
Peptide Sequence
GYSIFSYATK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
66
Peptide End
75
CPTAC ID
CPTAC-1478
Peptide Molecular Mass
1,135.5550
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 5.1 1.4 1.3 7.1 5.6 2.9 8.7 5.8 3.2 15 15 15
y6 (1+) 3.2 1.9 1.5 7.1 4.9 3.1 7.8 5.3 3.4 15 15 15
y5 (1+) 4.7 1.3 1.8 8.6 5.2 3.1 9.8 5.4 3.6 15 15 15
sum 2.8 1.4 1.1 7 5 2.8 7.5 5.2 3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-6237 Collapse assay details

Data source: Panorama

Official Gene Symbol
CRP
Peptide Sequence
APLTKPLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
7
Peptide Start
42
Peptide End
49
CPTAC ID
CPTAC-6237
Peptide Molecular Mass
866.5589
Species
Homo Sapiens
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Frozen tissue
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
Extended heavy peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent
Column Packing
Reprosil C18, 3um
Column Dimensions
75µm x 15cm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 37.9 21.4 34.5 44.9 23.7 50.5 58.8 31.9 61.2 15 15 14
y3 (1+) 22.9 5.7 12.3 37.2 15 14 43.7 16 18.6 15 15 14
y6 (2+) 12.6 6.6 9.2 15.2 15.2 12.1 19.7 16.6 15.2 15 15 14
y7 (2+) 12.6 4.2 8.6 16.2 10.5 13.5 20.5 11.3 16 15 15 14
sum 9.2 3.6 7.4 14 11.9 11.3 16.8 12.4 13.5 15 15 14


Additional Resources and Comments


Assay Details for CPTAC-6228 Collapse assay details

Data source: Panorama

Official Gene Symbol
CRP
Peptide Sequence
ESDTSYVSLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
7
Peptide Start
32
Peptide End
41
CPTAC ID
CPTAC-6228
Peptide Molecular Mass
1,127.5346
Species
Homo Sapiens
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Frozen tissue
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
Extended heavy peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent
Column Packing
Reprosil C18, 3um
Column Dimensions
75µm x 15cm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 5.7 3.1 6.1 5.8 7.1 7.3 8.1 7.7 9.5 15 15 15
y3 (1+) 6.3 1.2 4.5 8.9 5.9 6.4 10.9 6 7.8 15 15 15
y4 (1+) 5 2.1 5.4 10.2 6.8 6.6 11.4 7.1 8.5 15 15 15
y5 (1+) 5.3 0.9 3.1 7.3 6 4.8 9 6.1 5.7 15 15 15
y6 (1+) 4.1 2.1 3.8 7 5.3 5.4 8.1 5.7 6.6 15 15 15
sum 4.7 1.2 3.9 7.4 5.8 5.5 8.8 5.9 6.7 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3331 Collapse assay details

Data source: Panorama

Official Gene Symbol
Crp
Peptide Sequence
AFVFPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
27
Peptide End
32
CPTAC ID
non-CPTAC-3331
Peptide Molecular Mass
707.4006
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 3.4 3.9 7 12.7 7.4 7.7 13.1 8.4 10.4 15 15 15
y2 (1+) 5.8 8.6 7.5 14.5 8.3 10.9 15.6 12 13.2 15 15 15
y3 (1+) 7.4 6.6 5.8 12.6 7.8 8.5 14.6 10.2 10.3 15 15 15
y4 (1+) 5.3 5.3 5.6 12.6 7.3 6.6 13.7 9 8.7 15 15 15
y5 (1+) 7.4 4.4 9.8 16.7 7.9 9.9 18.3 9 13.9 15 15 15
sum 2.3 2.4 3.1 12.3 5.2 5.4 12.5 5.7 6.2 15 15 15


Additional Resources and Comments