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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
POGLUT1 C3orf9, CLP46, KTELC1, MDSRP
Sequence Length (AA) Molecular Weight (Da)
392 46189
Protein Name
Protein O-glucosyltransferase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEWWASSPLR LWLLLFLLPS AQGRQKESGS KWKVFIDQIN RSLENYEPCS
60 70 80 90 100
SQNCSCYHGV IEEDLTPFRG GISRKMMAEV VRRKLGTHYQ ITKNRLYREN
110 120 130 140 150
DCMFPSRCSG VEHFILEVIG RLPDMEMVIN VRDYPQVPKW MEPAIPVFSF
160 170 180 190 200
SKTSEYHDIM YPAWTFWEGG PAVWPIYPTG LGRWDLFRED LVRSAAQWPW
210 220 230 240 250
KKKNSTAYFR GSRTSPERDP LILLSRKNPK LVDAEYTKNQ AWKSMKDTLG
260 270 280 290 300
KPAAKDVHLV DHCKYKYLFN FRGVAASFRF KHLFLCGSLV FHVGDEWLEF
310 320 330 340 350
FYPQLKPWVH YIPVKTDLSN VQELLQFVKA NDDVAQEIAE RGSQFIRNHL
360 370 380 390 392
QMDDITCYWE NLLSEYSKFL SYNVTRRKGY DQIIPKMLKT EL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2230 Collapse assay details

Data source: Panorama

Official Gene Symbol
POGLUT1
Peptide Modified Sequence
N[+1.0]STAYFR
Modification Type
Deamidated (N)
Protein - Site of Modification
204
Peptide - Site of Modification
1
Peptide Start
204
Peptide End
210
CPTAC ID
CPTAC-2230
Peptide Molecular Mass
858.3872
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 7.3 3.3 3.1 9.2 3.9 3.3 11.7 5.1 4.5 15 15 15
y4 (1+) 4.2 1.8 2.6 4.7 2.6 2.8 6.3 3.2 3.8 15 15 15
y5 (1+) 6.8 4.3 2.5 7.2 4.4 2.5 9.9 6.2 3.5 15 15 15
sum 4.3 2.1 1.7 5.1 2.1 1.9 6.7 3 2.5 15 15 15


Additional Resources and Comments