MFAP4, CPTAC-2226 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MFAP4	N/A

Sequence Length (AA)	Molecular Weight (Da)
255	28648

Protein Name
Microfibril-associated glycoprotein 4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKALLALPLL	LLLSTPPCAP	QVSGIRGDAL	ERFCLQQPLD	CDDIYAQGYQ
60	70	80	90	100
SDGVYLIYPS	GPSVPVPVFC	DMTTEGGKWT	VFQKRFNGSV	SFFRGWNDYK
110	120	130	140	150
LGFGRADGEY	WLGLQNMHLL	TLKQKYELRV	DLEDFENNTA	YAKYADFSIS
160	170	180	190	200
PNAVSAEEDG	YTLFVAGFED	GGAGDSLSYH	SGQKFSTFDR	DQDLFVQNCA
210	220	230	240	250
ALSSGAFWFR	SCHFANLNGF	YLGGSHLSYA	NGINWAQWKG	FYYSLKRTEM
255
KIRRA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-2226 Collapse assay details

Data source: Panorama

Official Gene Symbol: MFAP4
Peptide Modified Sequence: VDLEDFEN[+1.0]NTAYAK
Modification Type: Deamidated (N)
Protein - Site of Modification: 137
Peptide - Site of Modification: 8
Peptide Start: 130
Peptide End: 143

CPTAC ID: CPTAC-2226
Peptide Molecular Mass: 1,628.7206
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G

CPTAC-2204: AOC1. LEN[+1.0]ITNPWSPR	CPTAC-2205: APOB. FEVDSPVYN[+1.0]ATWSASLK	CPTAC-2206: ATP1B3. LHVGYLQPLVAVQVSFAPN[+1.0]NTGK
CPTAC-2207: ADAMTS12. VGN[+1.0]WSK	CPTAC-2208: B3GNT2. DTFFN[+1.0]LSLK	CPTAC-2209: BMPR2. ISHEN[+1.0]GTILC[+57.0]SK
CPTAC-2210: CADM1. VDDEM[+16.0]PQHAVLSGPNLFINNLN[+1.0]K	CPTAC-2211: CDCP1. ESN[+1.0]ITVLIK	CPTAC-2212: FBLN5. N[+1.0]HTC[+57.0]NLQQTC[+57.0]YNLQGGFK
CPTAC-2213: FGL2. VAN[+1.0]LTFVVNSLDGK	CPTAC-2214: FGL2. LHVGNYN[+1.0]GTAGDALR	CPTAC-2215: FUCA1. N[+1.0]TTSVWYTSK
CPTAC-2216: GALNT5. AN[+1.0]TSLPFPK	CPTAC-2217: GGT1. LHNQLLPN[+1.0]VTTVER	CPTAC-2218: GNPTAB. TN[+1.0]VSHVVGQLDDIR
CPTAC-2219: GNPTG. YEFC[+57.0]PFHN[+1.0]VTQHEQTFR	CPTAC-2220: HYOU1. VFGSQN[+1.0]LTTVK	CPTAC-2221: IL1RAP. N[+1.0]TTYC[+57.0]SK
CPTAC-2222: LRP1. ELQGN[+1.0]C[+57.0]SR	CPTAC-2223: LRP1. DGTC[+57.0]IGN[+1.0]SSR	CPTAC-2224: GAA. QVVEN[+1.0]M[+16.0]TR
CPTAC-2225: MAN2B1. TN[+1.0]HTVM[+16.0]TM[+16.0]GSDFQYENANM[+16.0]WFK	CPTAC-2226: MFAP4. VDLEDFEN[+1.0]NTAYAK	CPTAC-2227: MUC16. TSSTEGN[+1.0]FTK
CPTAC-2228: NAGA. LGIYADM[+16.0]GN[+1.0]FTC[+57.0]M[+16.0]GYPGTTLDK	CPTAC-2229: P2RX7. TTN[+1.0]VSLYPGYNFR	CPTAC-2230: POGLUT1. N[+1.0]STAYFR
CPTAC-2231: GPLD1. N[+1.0]LTTSLTESVDR	CPTAC-2232: PLOD1. YIHQN[+1.0]YTK	CPTAC-2233: PON1. HAN[+1.0]WTLTPLK
CPTAC-2234: LTBR. AGHFQN[+1.0]TSSPSAR	CPTAC-2235: TLR8. GSYPQHIN[+1.0]ISR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	46.8	13.8	15.2	42	19.6	20.1	62.9	24	25.2	15	15	15
y7 (1+)	46.2	31.4	37	49.6	32.1	34.6	67.8	44.9	50.7	15	15	15
y8 (1+)	25.2	20.6	23.9	42.4	27	21.4	49.3	34	32.1	15	15	15
sum	24	10.6	13.3	23.3	13.5	12.3	33.4	17.2	18.1	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3363 Collapse assay details

Data source: Panorama

Official Gene Symbol: Mfap4
Peptide Sequence: GFYYSLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 242
Peptide End: 248

CPTAC ID: non-CPTAC-3363
Peptide Molecular Mass: 876.4382
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.