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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MFAP4 N/A
Sequence Length (AA) Molecular Weight (Da)
255 28648
Protein Name
Microfibril-associated glycoprotein 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKALLALPLL LLLSTPPCAP QVSGIRGDAL ERFCLQQPLD CDDIYAQGYQ
60 70 80 90 100
SDGVYLIYPS GPSVPVPVFC DMTTEGGKWT VFQKRFNGSV SFFRGWNDYK
110 120 130 140 150
LGFGRADGEY WLGLQNMHLL TLKQKYELRV DLEDFENNTA YAKYADFSIS
160 170 180 190 200
PNAVSAEEDG YTLFVAGFED GGAGDSLSYH SGQKFSTFDR DQDLFVQNCA
210 220 230 240 250
ALSSGAFWFR SCHFANLNGF YLGGSHLSYA NGINWAQWKG FYYSLKRTEM
255
KIRRA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2226 Collapse assay details

Data source: Panorama

Official Gene Symbol
MFAP4
Peptide Modified Sequence
VDLEDFEN[+1.0]NTAYAK
Modification Type
Deamidated (N)
Protein - Site of Modification
137
Peptide - Site of Modification
8
Peptide Start
130
Peptide End
143
CPTAC ID
CPTAC-2226
Peptide Molecular Mass
1,628.7206
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 46.8 13.8 15.2 42 19.6 20.1 62.9 24 25.2 15 15 15
y7 (1+) 46.2 31.4 37 49.6 32.1 34.6 67.8 44.9 50.7 15 15 15
y8 (1+) 25.2 20.6 23.9 42.4 27 21.4 49.3 34 32.1 15 15 15
sum 24 10.6 13.3 23.3 13.5 12.3 33.4 17.2 18.1 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3363 Collapse assay details

Data source: Panorama

Official Gene Symbol
Mfap4
Peptide Sequence
GFYYSLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
242
Peptide End
248
CPTAC ID
non-CPTAC-3363
Peptide Molecular Mass
876.4382
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-G


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 12.8 6.9 4.8 12.6 10.8 6.6 18 12.8 8.2 15 15 15
y2 (1+) 20.3 6.6 4.7 24.8 9.7 7.8 32 11.7 9.1 15 15 15
y3 (1+) 24.1 7.4 6.9 47.3 11.2 7.1 53.1 13.4 9.9 15 15 15
y4 (1+) 24.3 4.7 6.8 52.4 7.4 6.7 57.8 8.8 9.5 15 15 15
y5 (1+) 6.5 5.6 5.5 16.5 12.1 8.8 17.7 13.3 10.4 15 15 15
sum 9.2 3.7 3.7 18.4 10.4 5.6 20.6 11 6.7 15 15 15

Additional Resources and Comments