Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

FYN

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
FYN N/A
Sequence Length (AA) Molecular Weight (Da)
537 60762
Protein Name
Tyrosine-protein kinase Fyn
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGCVQCKDKE ATKLTEERDG SLNQSSGYRY GTDPTPQHYP SFGVTSIPNY
60 70 80 90 100
NNFHAAGGQG LTVFGGVNSS SHTGTLRTRG GTGVTLFVAL YDYEARTEDD
110 120 130 140 150
LSFHKGEKFQ ILNSSEGDWW EARSLTTGET GYIPSNYVAP VDSIQAEEWY
160 170 180 190 200
FGKLGRKDAE RQLLSFGNPR GTFLIRESET TKGAYSLSIR DWDDMKGDHV
210 220 230 240 250
KHYKIRKLDN GGYYITTRAQ FETLQQLVQH YSERAAGLCC RLVVPCHKGM
260 270 280 290 300
PRLTDLSVKT KDVWEIPRES LQLIKRLGNG QFGEVWMGTW NGNTKVAIKT
310 320 330 340 350
LKPGTMSPES FLEEAQIMKK LKHDKLVQLY AVVSEEPIYI VTEYMNKGSL
360 370 380 390 400
LDFLKDGEGR ALKLPNLVDM AAQVAAGMAY IERMNYIHRD LRSANILVGN
410 420 430 440 450
GLICKIADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVW
460 470 480 490 500
SFGILLTELV TKGRVPYPGM NNREVLEQVE RGYRMPCPQD CPISLHELMI
510 520 530 537
HCWKKDPEER PTFEYLQSFL EDYFTATEPQ YQPGENL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-1792 Collapse assay details

Data source: Panorama

Official Gene Symbol
FYN
Peptide Modified Sequence
LDNGGY[+80.0]YITTR
Modification Type
Phospho (Y)
Protein - Site of Modification
213
Peptide - Site of Modification
6
Peptide Start
208
Peptide End
218
CPTAC ID
CPTAC-1792
Peptide Molecular Mass
1,351.5809
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 13 11 10.7 24.8 11.8 11.4 28 16.1 15.6 15 15 15
y5 (1+) 16.2 8.1 9.6 25.5 7.6 10.5 30.2 11.1 14.2 15 15 15
y4 (1+) 35.2 5.4 7.3 35.7 10.4 8.4 50.1 11.7 11.1 15 15 15
y3 (1+) 11.7 5.7 6.9 14.4 5.7 6.5 18.6 8.1 9.5 15 15 15
y2 (1+) 47.4 20.7 14 52.6 20.7 13.2 70.8 29.3 19.2 15 15 15
b2 (1+) 39.3 10.6 13.9 37.7 11.8 16.7 54.5 15.9 21.7 15 15 15
y9 (1+) 44.5 9.5 7.7 42.8 15.1 12.7 61.7 17.8 14.9 15 15 15
y8 (1+) 27.9 6.3 7.3 32.5 4.8 6.7 42.8 7.9 9.9 15 15 15
y7 (1+) 26 6.5 7 27.5 7.6 7.9 37.8 10 10.6 15 15 15
sum 15.8 3.5 5.7 20 4.7 5.4 25.5 5.9 7.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments