FGL2, CPTAC-3274 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
FGL2	N/A

Sequence Length (AA)	Molecular Weight (Da)
441	49008

Protein Name
Uncharacterized protein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPPAGWCWLG	PVLAACGLLA	VADGAAEAGG	GAGAGAEAAC	PATLRSTGRC
60	70	80	90	100
AAGGECAYEV	RLPALTVQLP	AQWGRLEEVF	KEVQNLKETV	NSLKKSCQDC
110	120	130	140	150
KLQADDSRAP	GRGGQLSPSP	GAPGEAEDGR	VRELESEVNK	LSADLKDAKE
160	170	180	190	200
EIDVLHGRLE	ELSLVNMNNI	ENYVDSKVAN	LTSVVNSLDG	KCSSKCPSQE
210	220	230	240	250
HTQSRPVQHL	IYKDCSDYYT	IGKRSSETYR	VTPDPKNSSF	EVFCDMETMG
260	270	280	290	300
GGWTVLQARL	DGSTNFTRTW	QDYKVGFGNL	RREFWLGNDK	IHLLTKSKDM
310	320	330	340	350
ILRIDLEDFN	GVKLYALYDQ	FYVANEFLKY	RLHIGNYNGT	AGDALHFSKH
360	370	380	390	400
YNHDLKFFTT	PDRDNDRYPS	GNCGLYYSSG	WWFDACLSAN	LNGRYYHQKY
410	420	430	440	441
RGVRNGIFWG	TWPGISEAHP	GGYKSSLKEA	KMMIRPKHFK	P

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2214 Collapse assay details

Data source: Panorama

Official Gene Symbol: FGL2
Peptide Modified Sequence: LHVGNYN[+1.0]GTAGDALR
Modification Type: Deamidated (N)
Protein - Site of Modification: 336
Peptide - Site of Modification: 7
Peptide Start: 330
Peptide End: 344

CPTAC ID: CPTAC-2214
Peptide Molecular Mass: 1,557.7536
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: LCMS-8040
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Nexera X2 UHPLC
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	10.9	7.9	5.1	11.9	5.7	4.7	16.1	9.7	6.9	15	15	15
y5 (1+)	9.3	5.4	3.7	8.7	4	5.4	12.7	6.7	6.5	15	15	15
y3 (1+)	9.9	6.2	5.4	13.3	6.4	6.5	16.6	8.9	8.5	15	15	15
sum	7.8	4	3.7	8.1	2.2	3.7	11.2	4.6	5.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2213 Collapse assay details

Data source: Panorama

Official Gene Symbol: FGL2
Peptide Modified Sequence: VAN[+1.0]LTFVVNSLDGK
Modification Type: Deamidated (N)
Protein - Site of Modification: 179
Peptide - Site of Modification: 3
Peptide Start: 177
Peptide End: 190

CPTAC ID: CPTAC-2213
Peptide Molecular Mass: 1,476.7824
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang