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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Afm N/A
Sequence Length (AA) Molecular Weight (Da)
608 69379
Protein Name
Afamin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRHLKLTGFI FFLLPLTESL ALPTKPQDVD HFNATQKFID ENTTYLAIIA
60 70 80 90 100
FSQYVQEASF DEVETLVKVM LDYRDRCWAD NTLPECSKTA NDAIQDMLCD
110 120 130 140 150
MEGLPQKHNF SHCCGKAGFP RRLCFFYNKK ANVGFLPPFP TLDPEEKCQA
160 170 180 190 200
YKNNSESFLH LYMYEVARRN PFVFAPVLLA VAAWFEEAAT TCCEQQQKAT
210 220 230 240 250
CFQAKAAPIT QYLKASSSYQ RNVCGALIKF GPKVLNSINV AVFSKKFPKI
260 270 280 290 300
GFKDLTTLLE DVSSMYEGCC EGDVVHCIRS QSQVVNHICS KQDSISSKIK
310 320 330 340 350
VCCEKKTLER EACIINANKD DRPEGLSLRE AKFTESENVC QERDSDPDKF
360 370 380 390 400
FAEFIYEYSR RHPDLSTPEL LRITKVYMDF LEDCCSRENP AGCYRHVEDK
410 420 430 440 450
FNETTQRSLA MVQQECKQFQ ELGKDTLQRH FLVKFTKAAP QLPMEELVSL
460 470 480 490 500
SKEMVAALTT CCTLSDEFAC VDNLADLVLG ELCGVNTNRT INPAVDHCCK
510 520 530 540 550
TDFAFRRHCF EHLKADTTYE LPSVSALVSA LHTDWCQPRK EDLQNKKHRF
560 570 580 590 600
LVNLVKWMPG ITDEEWLCLF TKFTAAREEC SEVQEPESCF SPESSKTGDE
608
SQATEKQR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-649 Collapse assay details

Data source: Panorama

Official Gene Symbol
AFM
Peptide Modified Sequence
DIENFN[+1.0]STQK
Modification Type
Deamidated (NQ)
Protein - Site of Modification
33
Peptide - Site of Modification
6
Peptide Start
28
Peptide End
37
CPTAC ID
CPTAC-649
Peptide Molecular Mass
1,195.5356
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 5.2 4.5 3.5 6 4.7 5.3 7.9 6.5 6.4 15 15 15
y7 (1+) 7.1 6.3 7.2 8.9 6.5 8.1 11.4 9.1 10.8 15 15 15
y6 (1+) 7.6 5 5.7 9.4 6.7 6.2 12.1 8.4 8.4 15 15 15
sum 5.4 3.6 3.5 6.2 4.4 5 8.2 5.7 6.1 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-650 Collapse assay details

Data source: Panorama

Official Gene Symbol
AFM
Peptide Modified Sequence
FN[+1.0]ETTEK
Modification Type
Deamidated (NQ)
Protein - Site of Modification
402
Peptide - Site of Modification
2
Peptide Start
401
Peptide End
407
CPTAC ID
CPTAC-650
Peptide Molecular Mass
868.3814
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 30.9 23 37.1 45.3 23.2 52.8 54.8 32.7 64.5 14 15 15
y5 (1+) 58.7 44.7 32.4 82.8 47.7 30 101.5 65.4 44.2 13 15 14
y4 (1+) 23.9 70.8 21.7 53.5 86.4 34.8 58.6 111.7 41 10 15 14
sum 48.1 26.3 33.9 53.4 28.6 46.5 71.9 38.9 57.5 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3328 Collapse assay details

Data source: Panorama

Official Gene Symbol
Afm
Peptide Sequence
AAPITQYLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
206
Peptide End
214
CPTAC ID
non-CPTAC-3328
Peptide Molecular Mass
1,003.5702
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 2.6 2.4 3.8 13.8 6.2 12 14 6.6 12.6 15 15 15
y3 (1+) 13.7 5.4 4.1 17.5 6.4 6.5 22.2 8.4 7.7 15 15 15
y5 (1+) 3.5 2.4 4.2 12.4 4.8 5.4 12.9 5.4 6.8 15 15 15
y6 (1+) 4.1 4 3 15.9 6.8 5.7 16.4 7.9 6.4 15 15 15
y7 (1+) 4.1 2.8 4 14.3 5.1 10.9 14.9 5.8 11.6 15 15 15
sum 2.4 2.1 3.5 13.5 5.8 11 13.7 6.2 11.5 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1059 Collapse assay details

Data source: Panorama

Official Gene Symbol
AFM
Peptide Sequence
DADPDTFFAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
344
Peptide End
353
CPTAC ID
non-CPTAC-1059
Peptide Molecular Mass
1,125.4979
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 4.7 4.9 6.5 8.9 6.3 7.3 10.1 8 9.8 15 15 15
y7 (2+) 16.7 7.2 5.1 24.3 12.6 10.8 29.5 14.5 11.9 15 15 15
y8 (2+) 9.5 9.7 9 11.8 7.2 9.4 15.1 12.1 13 15 15 15
y7 (1+) 5.8 6.5 5 8.7 6.7 7 10.5 9.3 8.6 15 15 15
y8 (1+) 5.8 7 5.4 9.2 10 6.5 10.9 12.2 8.5 15 15 15
sum 5.1 2.6 3.9 9.6 5.7 4.9 10.9 6.3 6.3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1060 Collapse assay details

Data source: Panorama

Official Gene Symbol
AFM
Peptide Sequence
IAPQLSTEELVSLGEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
438
Peptide End
453
CPTAC ID
non-CPTAC-1060
Peptide Molecular Mass
1,712.9196
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 15 9.5 8.8 13.2 15 7.4 20 17.8 11.5 15 15 15
y14 (2+) 8.3 5.4 8.6 10.1 12.4 7.3 13.1 13.5 11.3 15 15 15
y3 (1+) 12.8 10.5 6.8 11.8 14.2 6.5 17.4 17.7 9.4 15 15 15
y5 (1+) 10.3 9.4 18.2 13.1 10.9 16.5 16.7 14.4 24.6 15 15 15
y11 (1+) 9.6 9.8 10.9 9.7 11.5 10.4 13.6 15.1 15.1 15 15 15
sum 8.2 5.7 8.6 9.3 12 7.6 12.4 13.3 11.5 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1058 Collapse assay details

Data source: Panorama

Official Gene Symbol
AFM
Peptide Sequence
LPNNVLQEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
89
Peptide End
97
CPTAC ID
non-CPTAC-1058
Peptide Molecular Mass
1,053.5819
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 7.4 5.2 4.7 8.3 7.7 5.4 11.1 9.3 7.2 15 15 15
y4 (1+) 14.3 5.4 3.9 22.4 7.6 3.8 26.6 9.3 5.4 14 15 15
y6 (1+) 19.8 10.4 6.3 20 11.8 9.8 28.1 15.7 11.7 15 15 15
y7 (1+) 9.2 7.5 5.1 10.6 7.6 5.4 14 10.7 7.4 15 15 15
y8 (1+) 15 8.1 8.1 18.7 8.8 7.5 24 12 11 14 15 15
sum 6.5 4.3 3.8 6.4 6.1 4.4 9.1 7.5 5.8 15 15 15


Additional Resources and Comments