Prdx1, CPTAC-3329 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Prdx1	Msp23, Paga, Tdpx2

Sequence Length (AA)	Molecular Weight (Da)
199	22176

Protein Name
Peroxiredoxin-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSSGNAKIGY	PAPNFKATAV	MPDGQFKDIS	LSEYKGKYVV	FFFYPLDFTF
60	70	80	90	100
VCPTEIIAFS	DRADEFKKLN	CQVIGASVDS	HFCHLAWINT	PKKQGGLGPM
110	120	130	140	150
NIPLISDPKR	TIAQDYGVLK	ADEGISFRGL	FIIDDKGILR	QITINDLPVG
160	170	180	190	199
RSVDEIIRLV	QAFQFTDKHG	EVCPAGWKPG	SDTIKPDVNK	SKEYFSKQK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3329 Collapse assay details

Data source: Panorama

Official Gene Symbol: Prdx1
Peptide Sequence: ADEGISFR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 121
Peptide End: 128

CPTAC ID: non-CPTAC-3329
Peptide Molecular Mass: 893.4243
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	17.1	10.5	6.4	20.1	12.9	10	26.4	16.6	11.9	15	15	15
y5 (1+)	10.2	6.4	7.5	15.3	11.3	7.5	18.4	13	10.6	15	15	15
y6 (1+)	13.3	8.2	7.1	14.9	11	7.5	20	13.7	10.3	15	15	15
y2 (1+)	29.9	9.1	7.8	31.8	11.6	9	43.6	14.7	11.9	15	15	15
y3 (1+)	12.6	5.2	5.9	13.1	10.2	6.2	18.2	11.4	8.6	15	15	15
sum	5.1	3.1	3.6	11.2	9.8	4.2	12.3	10.3	5.5	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3528 Collapse assay details

Data source: Panorama

Official Gene Symbol: Prdx1
Peptide Sequence: TIAQDYGVLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 111
Peptide End: 120

CPTAC ID: non-CPTAC-3528
Peptide Molecular Mass: 1,106.5972
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers