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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

C4b

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
C4b C4
Sequence Length (AA) Molecular Weight (Da)
1738 192915
Protein Name
Complement C4-B
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRLLWGLAWV FSFCASSLQK PRLLLFSPSV VNLGTPLSVG VQLLDAPPGQ
60 70 80 90 100
EVKGSVFLRN PKGGSCSPKK DFKLSSGDDF VLLSLEVPLE DVRSCGLFDL
110 120 130 140 150
RRAPHIQLVA QSPWLRNTAF KATETQGVNL LFSSRRGHIF VQTDQPIYNP
160 170 180 190 200
GQRVRYRVFA LDQKMRPSTD FLTITVENSH GLRVLKKEIF TSTSIFQDAF
210 220 230 240 250
TIPDISEPGT WKISARFSDG LESNRSTHFE VKKYVLPNFE VKITPWKPYI
260 270 280 290 300
LMVPSNSDEI QLDIQARYIY GKPVQGVAYT RFALMDEQGK RTFLRGLETQ
310 320 330 340 350
AKLVEGRTHI SISKDQFQAA LDKINIGVRD LEGLRLYAAT AVIESPGGEM
360 370 380 390 400
EEAELTSWRF VSSAFSLDLS RTKRHLVPGA HFLLQALVQE MSGSEASNVP
410 420 430 440 450
VKVSATLVSG SDSQVLDIQQ STNGIGQVSI SFPIPPTVTE LRLLVSAGSL
460 470 480 490 500
YPAIARLTVQ APPSRGTGFL SIEPLDPRSP SVGDTFILNL QPVGIPAPTF
510 520 530 540 550
SHYYYMIISR GQIMAMGREP RKTVTSVSVL VDHQLAPSFY FVAYFYHQGH
560 570 580 590 600
PVANSLLINI QSRDCEGKLQ LKVDGAKEYR NADMMKLRIQ TDSKALVALG
610 620 630 640 650
AVDMALYAVG GRSHKPLDMS KVFEVINSYN VGCGPGGGDD ALQVFQDAGL
660 670 680 690 700
AFSDGDRLTQ TREDLSCPKE KKSRQKRNVN FQKAVSEKLG QYSSPDAKRC
710 720 730 740 750
CQDGMTKLPM KRTCEQRAAR VPQQACREPF LSCCKFAEDL RRNQTRSQAH
760 770 780 790 800
LARNNHNMLQ EEDLIDEDDI LVRTSFPENW LWRVEPVDSS KLLTVWLPDS
810 820 830 840 850
MTTWEIHGVS LSKSKGLCVA KPTRVRVFRK FHLHLRLPIS IRRFEQFELR
860 870 880 890 900
PVLYNYLNDD VAVSVHVTPV EGLCLAGGGM MAQQVTVPAG SARPVAFSVV
910 920 930 940 950
PTAAANVPLK VVARGVFDLG DAVSKILQIE KEGAIHREEL VYNLDPLNNL
960 970 980 990 1000
GRTLEIPGSS DPNIVPDGDF SSLVRVTASE PLETMGSEGA LSPGGVASLL
1010 1020 1030 1040 1050
RLPQGCAEQT MIYLAPTLTA SNYLDRTEQW SKLSPETKDH AVDLIQKGYM
1060 1070 1080 1090 1100
RIQQFRKNDG SFGAWLHRDS STWLTAFVLK ILSLAQEQVG NSPEKLQETA
1110 1120 1130 1140 1150
SWLLAQQLGD GSFHDPCPVI HRAMQGGLVG SDETVALTAF VVIALHHGLD
1160 1170 1180 1190 1200
VFQDDDAKQL KNRVEASITK ANSFLGQKAS AGLLGAHAAA ITAYALTLTK
1210 1220 1230 1240 1250
ASEDLRNVAH NSLMAMAEET GEHLYWGLVL GSQDKVVLRP TAPRSPTEPV
1260 1270 1280 1290 1300
PQAPALWIET TAYALLHLLL REGKGKMADK AASWLTHQGS FHGAFRSTQD
1310 1320 1330 1340 1350
TVVTLDALSA YWIASHTTEE KALNVTLSSM GRNGLKTHGL HLNNHQVKGL
1360 1370 1380 1390 1400
EEELKFSLGS TISVKVEGNS KGTLKILRTY NVLDMKNTTC QDLQIEVKVT
1410 1420 1430 1440 1450
GAVEYAWDAN EDYEDYYDMP AADDPSVPLQ PVTPLQLFEG RRSRRRREAP
1460 1470 1480 1490 1500
KVVEEQESRV QYTVCIWRNG KLGLSGMAIA DITLLSGFHA LRADLEKLTS
1510 1520 1530 1540 1550
LSDRYVSHFE TDGPHVLLYF DSVPTTRECV GFGASQEVVV GLVQPSSAVL
1560 1570 1580 1590 1600
YDYYSPDHKC SVFYAAPTKS QLLATLCSGD VCQCAEGKCP RLLRSLERRV
1610 1620 1630 1640 1650
EDKDGYRMRF ACYYPRVEYG FTVKVLREDG RAAFRLFESK ITQVLHFRKD
1660 1670 1680 1690 1700
TMASIGQTRN FLSRASCRLR LEPNKEYLIM GMDGETSDNK GDPQYLLDSN
1710 1720 1730 1738
TWIEEMPSEQ MCKSTRHRAA CFQLKDFLME FSSRGCQV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3339 Collapse assay details

Data source: Panorama

Official Gene Symbol
C4b
Peptide Sequence
DHAVDLIQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1039
Peptide End
1047
CPTAC ID
non-CPTAC-3339
Peptide Molecular Mass
1,037.5506
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 6.1 4.7 3.1 14 5.9 5.5 15.3 7.5 6.3 15 15 15
b5 (1+) 3.3 4.7 4.9 15.1 7.2 6.6 15.5 8.6 8.2 15 15 15
b3 (1+) 4.6 4 3.1 12.6 6.9 8.5 13.4 8 9 15 15 15
b2 (1+) 3.5 4.1 3.5 13.1 4.3 5 13.6 5.9 6.1 15 15 15
y7 (1+) 6.3 4.4 5 12.4 6.3 11.1 13.9 7.7 12.2 15 15 15
sum 2.8 2.1 1.8 12.6 4 5 12.9 4.5 5.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments