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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ppa1 Pp, Pyp
Sequence Length (AA) Molecular Weight (Da)
289 32667
Protein Name
Inorganic pyrophosphatase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSGFSSEERA APFTLEYRVF LKNEKGQYIS PFHDVPIYAD KDVFHMVVEV
60 70 80 90 100
PRWSNAKMEI ATKDPLNPIK QDVKKGKLRY VANLFPYKGY IWNYGAIPQT
110 120 130 140 150
WEDPGHSDKH TGCCGDNDPI DVCEIGSKVC ARGEIIRVKV LGILAMIDEG
160 170 180 190 200
ETDWKVIAIN VDDPDAANYK DISDVERLKP GYLEATVDWF RRYKVPDGKP
210 220 230 240 250
ENEFAFNAEF KNKDFAVDII KSTHDYWKAL VTKKTDGKGI SCMNTTVSES
260 270 280 289
PFKCDPDAAK AIVDALPPPC ESACSLPTDV DKWFHQQKN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3923 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ppa1
Peptide Sequence
AAPFTLEYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
10
Peptide End
18
CPTAC ID
non-CPTAC-3923
Peptide Molecular Mass
1,066.5447
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 13.7 6 3.7 17.5 9.4 6.7 22.2 11.2 7.7 15 15 15
y6 (1+) 11.6 3.7 2.9 14.7 7.9 7.3 18.7 8.7 7.9 15 15 15
y5 (1+) 9.8 3.9 4.3 6.7 4.8 9.6 11.9 6.2 10.5 15 15 15
y2 (1+) 16.4 7.6 4.8 25.8 7.8 7.7 30.6 10.9 9.1 15 15 15
y7 (2+) 5.6 4.6 2.9 8.9 4.9 6.5 10.5 6.7 7.1 15 15 15
sum 4.6 4.1 2.4 8.6 4 6.6 9.8 5.7 7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3340 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ppa1
Peptide Sequence
DISDVER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
171
Peptide End
177
CPTAC ID
non-CPTAC-3340
Peptide Molecular Mass
832.3927
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 6 5.5 4.3 13.9 7.9 6.5 15.1 9.6 7.8 15 15 15
y3 (2+) 113.1 41.4 64.3 172.8 156.3 76.3 206.5 161.7 99.8 15 15 15
y3 (1+) 13.2 9.1 5.5 30 9.7 6.3 32.8 13.3 8.4 15 15 15
y4 (1+) 14.1 6.1 5.7 57.7 8.2 9.4 59.4 10.2 11 15 15 15
y5 (1+) NA NA NA NA NA NA NA NA NA 15 15 15
sum 5 3.7 3.4 23.7 10.8 4.2 24.2 11.4 5.4 15 15 15

Additional Resources and Comments