Eno1, CPTAC-3348 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Eno1	Eno-1

Sequence Length (AA)	Molecular Weight (Da)
434	47141

Protein Name
Alpha-enolase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSILRIHARE	IFDSRGNPTV	EVDLYTAKGL	FRAAVPSGAS	TGIYEALELR
60	70	80	90	100
DNDKTRFMGK	GVSQAVEHIN	KTIAPALVSK	KVNVVEQEKI	DKLMIEMDGT
110	120	130	140	150
ENKSKFGANA	ILGVSLAVCK	AGAVEKGVPL	YRHIADLAGN	PEVILPVPAF
160	170	180	190	200
NVINGGSHAG	NKLAMQEFMI	LPVGASSFRE	AMRIGAEVYH	NLKNVIKEKY
210	220	230	240	250
GKDATNVGDE	GGFAPNILEN	KEALELLKTA	IAKAGYTDQV	VIGMDVAASE
260	270	280	290	300
FYRSGKYDLD	FKSPDDPSRY	ITPDQLADLY	KSFVQNYPVV	SIEDPFDQDD
310	320	330	340	350
WGAWQKFTAS	AGIQVVGDDL	TVTNPKRIAK	AASEKSCNCL	LLKVNQIGSV
360	370	380	390	400
TESLQACKLA	QSNGWGVMVS	HRSGETEDTF	IADLVVGLCT	GQIKTGAPCR
410	420	430	434
SERLAKYNQI	LRIEEELGSK	AKFAGRSFRN	PLAK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-70 Collapse assay details

Data source: Panorama

Official Gene Symbol: ENO1
Peptide Sequence: YISPDQLADLYK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 270
Peptide End: 281

CPTAC ID: CPTAC-70
Peptide Molecular Mass: 1,424.7187
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: cell line lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 5500 QTRAP (ABSCIEX)
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C,15N

LC: nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing: ChromXP C18-CL, 3 um, 120A
Column Dimensions: 150 x 0.075 mm
Flow Rate: 500 nL / min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	15.8	7.5	4.5	19	9.3	5.6	24.7	11.9	7.2	15	25	15
y9 (1+)	14.7	6.9	6.9	15.6	7.5	7.8	21.4	10.2	10.4	15	25	15
y7 (1+)	37.4	7.2	4.3	47.4	9.3	6.3	60.4	11.8	7.6	15	25	15
sum	11.6	6.7	5.2	15.2	8.1	6.2	19.1	10.5	8.1	15	25	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3347 Collapse assay details

Data source: Panorama

Official Gene Symbol: Eno1
Peptide Sequence: EIFDSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 10
Peptide End: 15

CPTAC ID: non-CPTAC-3347
Peptide Molecular Mass: 765.3657
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b4 (2+)	86.6	50.2	35.1	81.3	63.9	42.5	118.8	81.3	55.1	15	15	15
b2 (1+)	9	5.7	7.8	13.2	6.8	9.3	16	8.9	12.1	15	15	15
y5 (2+)	25.3	13	13.2	29.8	15.4	13.7	39.1	20.2	19	15	15	15
y3 (1+)	6.7	11.4	9.8	15.9	14.6	11.6	17.3	18.5	15.2	15	15	15
y4 (1+)	8.3	7.2	6.9	11.8	8.3	9.2	14.4	11	11.5	15	15	15
sum	6.3	7.7	6.5	11.1	8.6	7.1	12.8	11.5	9.6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3348 Collapse assay details

Data source: Panorama

Official Gene Symbol: Eno1
Peptide Sequence: TIAPALVSK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 72
Peptide End: 80

CPTAC ID: non-CPTAC-3348
Peptide Molecular Mass: 898.5488
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	4.9	4	4.4	13.6	9.6	4.5	14.5	10.4	6.3	15	15	15
b2 (1+)	4.3	3.4	5.7	11.9	7	8.6	12.7	7.8	10.3	15	15	15
y6 (1+)	3	5.7	4.2	11.2	6.8	5.7	11.6	8.9	7.1	15	15	15
y7 (1+)	4.2	4.6	5.8	12.4	8.8	6.6	13.1	9.9	8.8	15	15	15
sum	2.2	2.4	3.9	11.6	7.2	5.6	11.8	7.6	6.8	15	15	15

Search form

Eno1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-70 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for non-CPTAC-3347 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for non-CPTAC-3348 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments