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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Cd300lg Clm9, D11Ertd736e
Sequence Length (AA) Molecular Weight (Da)
331 36756
Protein Name
CMRF35-like molecule 9
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRPLVLLWGC LVLPGYEALK GPKEISGFEG DTVSLRCTYV EKMKEHRKYW
60 70 80 90 100
CRQGGILVSR CGDIVYANQD QEVTRGRMSI RDSPQELSMT VIMRDLTLKD
110 120 130 140 150
SGKYWCGIDR LGRDESFEVT LIVFPGKRDN AVPAGTCCPS SPTPSFQPLT
160 170 180 190 200
PTRSLQPKAK AWQTQLPEPR SSRPVVWLPL TTPQDSRAVA SSVSKPSVSI
210 220 230 240 250
PMVRMMAPVL ILLSLLLAAG LIAFGSHMLR WRKKAWLATE TQKNEKVYLE
260 270 280 290 300
TSLPGNGWTT EDSTIDLAVT PECLRNLNPS AVPSPETQNL SQSTEEEEAA
310 320 330 331
RSLDDDKEDV MAPPPLQMSA EELAFSEFIS V

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3349 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cd300lg
Peptide Sequence
EISGFEGDTVSLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
24
Peptide End
36
CPTAC ID
non-CPTAC-3349
Peptide Molecular Mass
1,408.6834
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (2+) 8.4 5.6 10.9 15.4 6.1 11.1 17.5 8.3 15.6 15 15 15
y3 (1+) 5.9 3.5 7 15.2 8.5 7.7 16.3 9.2 10.4 15 15 15
y7 (1+) 6.7 3.7 5.6 17 5 6.5 18.3 6.2 8.6 15 15 15
y8 (1+) 6.3 4.3 7.3 14.8 6.5 8.5 16.1 7.8 11.2 15 15 15
y11 (1+) 5.6 3.3 3.4 18.4 9.2 6.1 19.2 9.8 7 15 15 15
sum 4.1 1.3 1.6 15.1 5.8 3.6 15.6 5.9 3.9 15 15 15


Additional Resources and Comments