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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Cd248 Tem1
Sequence Length (AA) Molecular Weight (Da)
765 81813
Protein Name
Endosialin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLLRLLLAWV AAVPALGQVP WTPEPRAACG PSSCYALFPR RRTFLEAWRA
60 70 80 90 100
CRELGGNLAT PRTPEEAQRV DSLVGVGPAN GLLWIGLQRQ ARQCQPQRPL
110 120 130 140 150
RGFIWTTGDQ DTAFTNWAQP ATEGPCPAQR CAALEASGEH RWLEGSCTLA
160 170 180 190 200
VDGYLCQFGF EGACPALPLE VGQAGPAVYT TPFNLVSSEF EWLPFGSVAA
210 220 230 240 250
VQCQAGRGAS LLCVKQPSGG VGWSQTGPLC PGTGCGPDNG GCEHECVEEV
260 270 280 290 300
DGAVSCRCSE GFRLAADGHS CEDPCAQAPC EQQCEPGGPQ GYSCHCRLGF
310 320 330 340 350
RPAEDDPHRC VDTDECQIAG VCQQMCVNYV GGFECYCSEG HELEADGISC
360 370 380 390 400
SPAGAMGAQA SQDLRDELLD DGEEGEDEEE PWEDFDGTWT EEQGILWLAP
410 420 430 440 450
THPPDFGLPY RPNFPQDGEP QRLHLEPTWP PPLSAPRGPY HSSVVSATRP
460 470 480 490 500
MVISATRPTL PSAHKTSVIS ATRPPLSPVH PPAMAPATPP AVFSEHQIPK
510 520 530 540 550
IKANYPDLPF GHKPGITSAT HPARSPPYQP PIISTNYPQV FPPHQAPMSP
560 570 580 590 600
DTHTITYLPP VPPHLDPGDT TSKAHQHPLL PDAPGIRTQA PQLSVSALQP
610 620 630 640 650
PLPTNSRSSV HETPVPAANQ PPAFPSSPLP PQRPTNQTSS ISPTHSYSRA
660 670 680 690 700
PLVPREGVPS PKSVPQLPSV PSTAAPTALA ESGLAGQSQR DDRWLLVALL
710 720 730 740 750
VPTCVFLVVL LALGIVYCTR CGSHAPNKRI TDCYRWVTHA GNKSSTEPMP
760 765
PRGSLTGVQT CRTSV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3350 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cd248
Peptide Sequence
ELGGNLATPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
53
Peptide End
62
CPTAC ID
non-CPTAC-3350
Peptide Molecular Mass
1,026.5458
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 9.6 4.4 7.2 17.8 8.3 7.8 20.2 9.4 10.6 15 15 15
y3 (1+) 7.8 4.7 4.2 14.9 10 5.1 16.8 11 6.6 15 15 15
y4 (1+) 5.9 5 5 18 9.9 6 18.9 11.1 7.8 15 15 15
y5 (1+) 4.9 7.2 6.2 19 10.9 6.7 19.6 13.1 9.1 15 15 15
y8 (1+) 8.9 6.5 6.3 14 9.4 6.7 16.6 11.4 9.2 15 15 15
sum 4.3 1.2 2.4 14.8 8.2 2.9 15.4 8.3 3.8 15 15 15


Additional Resources and Comments