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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Comp N/A
Sequence Length (AA) Molecular Weight (Da)
755 82342
Protein Name
Cartilage oligomeric matrix protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGPTACVLVL ALAILRATGQ GQIPLGGDLA PQMLRELQET NAALQDVREL
60 70 80 90 100
LRQQVKEITF LKNTVMECDA CGMQPARTPG LSVRPVPLCA PGSCFPGVVC
110 120 130 140 150
SETATGARCG PCPPGYTGNG SHCTDVNECN AHPCFPRVRC INTSPGFHCE
160 170 180 190 200
ACPPGFSGPT HEGVGLTFAK SNKQVCTDIN ECETGQHNCV PNSVCVNTRG
210 220 230 240 250
SFQCGPCQPG FVGDQTSGCQ RRGQHFCPDG SPSPCHEKAN CVLERDGSRS
260 270 280 290 300
CVCAVGWAGN GLLCGRDTDL DGFPDEKLRC SERQCRKDNC VTVPNSGQED
310 320 330 340 350
VDRDGIGDAC DPDADGDGVP NEQDNCPLVR NPDQRNSDSD KWGDACDNCR
360 370 380 390 400
SKKNDDQKDT DLDGRGDACD DDIDGDRIRN VADNCPRVPN FDQSDSDGDG
410 420 430 440 450
VGDACDNCPQ KDNPDQRDVD HDFVGDACDS DQDQDGDGHQ DSRDNCPTVP
460 470 480 490 500
NSAQQDSDHD GKGDACDDDD DNDGVPDSRD NCRLVPNPGQ EDNDRDGVGD
510 520 530 540 550
ACQGDFDADK VIDKIDVCPE NAEVTLTDFR AFQTVVLDPE GDAQIDPNWV
560 570 580 590 600
VLNQGMEIVQ TMNSDPGLAV GYTAFNGVDF EGTFHVNTAT DDDYAGFIFG
610 620 630 640 650
YQDSSSFYVV MWKQMEQTYW QANPFRAVAE PGIQLKAVKS STGPGEQLRN
660 670 680 690 700
ALWHTGDTAS QVRLLWKDPR NVGWKDKTSY RWFLQHRPQV GYIRVRFYEG
710 720 730 740 750
PELVADSNVV LDTAMRGGRL GVFCFSQENI IWANLRYRCN DTIPEDYESH
755
RLQRV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3351 Collapse assay details

Data source: Panorama

Official Gene Symbol
Comp
Peptide Sequence
ELQETNAALQDVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
36
Peptide End
48
CPTAC ID
non-CPTAC-3351
Peptide Molecular Mass
1,485.7423
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 10.9 6.7 2.9 13.5 9.3 5.9 17.4 11.5 6.6 15 15 15
y8 (1+) 10.6 7.5 7.1 14.1 10.6 8.7 17.6 13 11.2 15 15 15
y6 (1+) 12.5 5 6.2 20.6 9.7 5.6 24.1 10.9 8.4 15 15 15
y4 (1+) 9.3 4.1 7.2 14.2 8.2 9.1 17 9.2 11.6 15 15 15
y2 (1+) 7.4 4.1 5.6 15.1 8.8 6.3 16.8 9.7 8.4 15 15 15
sum 3.6 2 2.6 12.9 7.8 4.4 13.4 8.1 5.1 15 15 15


Additional Resources and Comments