Ggh, CPTAC-3352 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Ggh	N/A

Sequence Length (AA)	Molecular Weight (Da)
317	35470

Protein Name
Gamma-glutamyl hydrolase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MANLGYLLCL	LGLLLCGLSS	PGMSRPYNHG	SERPIIGVVM	QECFGKMAKL
60	70	80	90	100
GNYYIAASYV	KYIESAGARV	VPIRPDLSDA	EYEELFRSIN	GVLLPGGGAN
110	120	130	140	150
LTDSGYSRVA	KIFFSKALES	FDNGDHFPVW	GTCLGFEELS	VLVSGENLLT
160	170	180	190	200
STDTKSKKLP	LNFTEGARKS	RMFKHFPTEL	LDSLALENLT	ANFHKWSLSV
210	220	230	240	250
KNFTENEKLK	KFFNILTTNT	DGKTEFISSM	EGFKYPVYAV	QWHPEKAAFE
260	270	280	290	300
WKNLGGISHA	PNAVKTSFYL	AEFLVSEARK	NSHHFENVVK	ETASLIYKFN
310	317
PIYTGNISSF	QQAYMFD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3353 Collapse assay details

Data source: Panorama

Official Gene Symbol: Ggh
Peptide Sequence: ETASLIYK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 291
Peptide End: 298

CPTAC ID: non-CPTAC-3353
Peptide Molecular Mass: 923.4964
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y2 (1+)	10.3	7.1	7	13.4	10.4	8.5	16.9	12.6	11	15	15	15
y3 (1+)	15	6.5	10	17	12.2	10.8	22.7	13.8	14.7	15	15	15
y4 (1+)	10	9.4	7.8	22.1	8.6	10.8	24.3	12.7	13.3	15	15	15
y5 (1+)	11.1	8.1	9.9	14.5	15.6	9.9	18.3	17.6	14	15	15	15
y6 (1+)	10.8	5.4	9	15.4	9.5	8.9	18.8	10.9	12.7	15	15	15
sum	3.1	3.1	2.8	12.6	9.7	4.8	13	10.2	5.6	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3352 Collapse assay details

Data source: Panorama

Official Gene Symbol: Ggh
Peptide Sequence: YIESAGAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 62
Peptide End: 69

CPTAC ID: non-CPTAC-3352
Peptide Molecular Mass: 865.4294
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.