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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ggh N/A
Sequence Length (AA) Molecular Weight (Da)
317 35470
Protein Name
Gamma-glutamyl hydrolase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MANLGYLLCL LGLLLCGLSS PGMSRPYNHG SERPIIGVVM QECFGKMAKL
60 70 80 90 100
GNYYIAASYV KYIESAGARV VPIRPDLSDA EYEELFRSIN GVLLPGGGAN
110 120 130 140 150
LTDSGYSRVA KIFFSKALES FDNGDHFPVW GTCLGFEELS VLVSGENLLT
160 170 180 190 200
STDTKSKKLP LNFTEGARKS RMFKHFPTEL LDSLALENLT ANFHKWSLSV
210 220 230 240 250
KNFTENEKLK KFFNILTTNT DGKTEFISSM EGFKYPVYAV QWHPEKAAFE
260 270 280 290 300
WKNLGGISHA PNAVKTSFYL AEFLVSEARK NSHHFENVVK ETASLIYKFN
310 317
PIYTGNISSF QQAYMFD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3353 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ggh
Peptide Sequence
ETASLIYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
291
Peptide End
298
CPTAC ID
non-CPTAC-3353
Peptide Molecular Mass
923.4964
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 10.3 7.1 7 13.4 10.4 8.5 16.9 12.6 11 15 15 15
y3 (1+) 15 6.5 10 17 12.2 10.8 22.7 13.8 14.7 15 15 15
y4 (1+) 10 9.4 7.8 22.1 8.6 10.8 24.3 12.7 13.3 15 15 15
y5 (1+) 11.1 8.1 9.9 14.5 15.6 9.9 18.3 17.6 14 15 15 15
y6 (1+) 10.8 5.4 9 15.4 9.5 8.9 18.8 10.9 12.7 15 15 15
sum 3.1 3.1 2.8 12.6 9.7 4.8 13 10.2 5.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3352 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ggh
Peptide Sequence
YIESAGAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
62
Peptide End
69
CPTAC ID
non-CPTAC-3352
Peptide Molecular Mass
865.4294
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 25.2 8.4 12.7 28.8 13.7 11.5 38.3 16.1 17.1 15 15 15
y6 (1+) 7.9 6.7 10.5 16.8 10 13.3 18.6 12 16.9 15 15 15
y5 (1+) 18.7 8.3 12.7 22.8 11.9 13.4 29.5 14.5 18.5 15 15 15
y4 (1+) 34 9.7 13.2 35.5 13.2 12.5 49.2 16.4 18.2 15 15 15
b2 (1+) 10.3 9.5 10.6 16.5 11.1 9.3 19.5 14.6 14.1 15 15 15
sum 5.8 3.8 6.1 14.2 8.6 8.9 15.3 9.4 10.8 15 15 15


Additional Resources and Comments