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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Itih3 N/A
Sequence Length (AA) Molecular Weight (Da)
889 99358
Protein Name
Inter-alpha-trypsin inhibitor heavy chain H3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRTMWWPCLV LALLSGLETS GFPRSPLQLL GKRSLPEGVV DGIEVYSTKI
60 70 80 90 100
SCKVTSRFAH NVVTTRAVNR ADTAKEVSFD VELPKTAFIT NFTLTIDGVT
110 120 130 140 150
YPGNVKEKEV AQKQYEKAVS QGKTAGLVKA SGRKLEKFTV SVNVAAGSKV
160 170 180 190 200
TFELTYEELL KRNKGKYEMY LKVQPKQLVR HFEIDAHIFE PQGISMLDAE
210 220 230 240 250
ASFITNDLLG SALTKSFSGK KGHVSFKPSL DQQRSCPTCT DSLLNGDFTI
260 270 280 290 300
VYDVNRESPG NVQIVNGYFV HFFAPQGLPV VPKNIVFVID VSGSMSGRKI
310 320 330 340 350
QQTREALLKI LDDVKEDDYL NFILFSTDVT TWKDHLVQAT PANLKEAKTF
360 370 380 390 400
VKNIHDQSMT NINDGLLKGI EMLNKAREDH TVPERSTSII IMLTDGDANT
410 420 430 440 450
GESRPEKIQE NVRNAIGGKF PLYNLGFGNN LNYNFLETLA LENHGLARRI
460 470 480 490 500
YEDSDANLQL QGFYEEVANP LLTNVEVEYP ENAILDLTRN SYPHFYDGSE
510 520 530 540 550
IVVAGRLVDR NMDNFKADVK GHGALNDLTF TEEVDMEEMD AALKEQGYIF
560 570 580 590 600
GDYIERLWAY LTIEQLLEKR KNAKGDEKEN ITAEALDLSL KYHFVTPLTS
610 620 630 640 650
MVVTKPEDNE DQTSIADKPG EEAIAETTTM SFLTTQQSSQ SPYYYVDGDP
660 670 680 690 700
HFIIQIPGKN DSICFNIDEK PGTVLRLIQD PVTGITVTGQ IIGDKRSNAS
710 720 730 740 750
SRTGKTYFGK LGITNAWMDF RVEVTTEKII LGTGAELSTF SWLDTITVTQ
760 770 780 790 800
TGLSVTINRK KNMVVSFGDG ISFVIILHQV WKKHPVHQDF LGFYVVDSHR
810 820 830 840 850
MSAQTHGLLG QFFQPFDFKV FGIRPGSDPT KPDATMVVKN HRLTVTRGSQ
860 870 880 889
KDYRKDASVG TKVICWFVHN NGEGLIDGVH TDYIVPSLF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3354 Collapse assay details

Data source: Panorama

Official Gene Symbol
Itih3
Peptide Sequence
EVSFDVELPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
76
Peptide End
85
CPTAC ID
non-CPTAC-3354
Peptide Molecular Mass
1,161.5918
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 4.4 4.8 2.3 12.7 4.8 5.2 13.4 6.8 5.7 15 15 15
y8 (2+) 5 4.3 4 14.8 8.2 5.6 15.6 9.3 6.9 15 15 15
y2 (1+) 4.9 5.1 2 12.1 8.3 7.1 13.1 9.7 7.4 15 15 15
y6 (1+) 3.7 5.9 4 14 7.7 6.2 14.5 9.7 7.4 15 15 15
y8 (1+) 2.3 4.6 4.4 14.4 5.8 6.3 14.6 7.4 7.7 15 15 15
sum 1.9 2.1 1.6 13.3 5.2 5.1 13.4 5.6 5.3 15 15 15


Additional Resources and Comments