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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Orm2 Agp-2, Orm-2
Sequence Length (AA) Molecular Weight (Da)
207 23843
Protein Name
Alpha-1-acid glycoprotein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MALHMILVMV SLLPLLEAQN PEHVNITIGD PITNETLSWL SDKWFFIGAA
60 70 80 90 100
VLNPDYRQEI QKTQMVFFNL TPNLINDTME LREYHTIDDH CVYNSTHLGI
110 120 130 140 150
QRENGTLSKY VGGVKIFADL IVLKMHGAFM LAFDLKDEKK RGLSLNAKRP
160 170 180 190 200
DITPELREVF QKAVTHVGMD ESEIIFVDWK KDRCSQQEKQ QLELEKETKK
207
DPEEGQA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3368 Collapse assay details

Data source: Panorama

Official Gene Symbol
Orm2
Peptide Sequence
GLSLNAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
142
Peptide End
148
CPTAC ID
non-CPTAC-3368
Peptide Molecular Mass
701.4072
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 9.4 11.6 5.4 14.6 11.6 7.9 17.4 16.4 9.6 15 15 15
y4 (1+) 4.4 7 11.8 13.3 12.2 10.3 14 14.1 15.7 15 15 15
y3 (1+) 9.8 10.8 8.5 14.3 11.2 7.5 17.3 15.6 11.3 15 15 15
y6 (2+) 10.6 7.6 7 22.4 13.6 6.5 24.8 15.6 9.6 15 15 15
y5 (2+) 16.5 8.8 6.9 15.2 11.3 7.2 22.4 14.3 10 15 15 15
sum 7.8 9.8 4.9 13.4 10 7.3 15.5 14 8.8 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3307 Collapse assay details

Data source: Panorama

Official Gene Symbol
Orm2
Peptide Sequence
IFADLIVLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
116
Peptide End
124
CPTAC ID
non-CPTAC-3307
Peptide Molecular Mass
1,030.6427
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 3.7 3.4 2.9 14.6 4.1 3.9 15.1 5.3 4.9 15 15 15
y8 (1+) 4.1 4.3 4.2 13.8 6.4 4.9 14.4 7.7 6.5 15 15 15
b2 (1+) 2.8 3.2 3.7 9.9 4.2 5.8 10.3 5.3 6.9 15 15 15
y6 (1+) 4 3.4 3.5 12.5 3.9 5.6 13.1 5.2 6.6 15 15 15
y3 (1+) 4.6 2.7 4.7 14.1 3.4 6.7 14.8 4.3 8.2 15 15 15
sum 2 2.2 1.9 12.5 3.7 3 12.7 4.3 3.6 15 15 15


Additional Resources and Comments