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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Pcsk9 Narc1
Sequence Length (AA) Molecular Weight (Da)
694 74823
Protein Name
Proprotein convertase subtilisin/kexin type 9
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGTHCSAWLR WPLLPLLPPL LLLLLLLCPT GAGAQDEDGD YEELMLALPS
60 70 80 90 100
QEDGLADEAA HVATATFRRC SKEAWRLPGT YIVVLMEETQ RLQIEQTAHR
110 120 130 140 150
LQTRAARRGY VIKVLHIFYD LFPGFLVKMS SDLLGLALKL PHVEYIEEDS
160 170 180 190 200
FVFAQSIPWN LERIIPAWHQ TEEDRSPDGS SQVEVYLLDT SIQGAHREIE
210 220 230 240 250
GRVTITDFNS VPEEDGTRFH RQASKCDSHG THLAGVVSGR DAGVAKGTSL
260 270 280 290 300
HSLRVLNCQG KGTVSGTLIG LEFIRKSQLI QPSGPLVVLL PLAGGYSRIL
310 320 330 340 350
NAACRHLART GVVLVAAAGN FRDDACLYSP ASAPEVITVG ATNAQDQPVT
360 370 380 390 400
LGTLGTNFGR CVDLFAPGKD IIGASSDCST CFMSQSGTSQ AAAHVAGIVA
410 420 430 440 450
RMLSREPTLT LAELRQRLIH FSTKDVINMA WFPEDQQVLT PNLVATLPPS
460 470 480 490 500
THETGGQLLC RTVWSAHSGP TRTATATARC APEEELLSCS SFSRSGRRRG
510 520 530 540 550
DWIEAIGGQQ VCKALNAFGG EGVYAVARCC LVPRANCSIH NTPAARAGLE
560 570 580 590 600
THVHCHQKDH VLTGCSFHWE VEDLSVRRQP ALRSRRQPGQ CVGHQAASVY
610 620 630 640 650
ASCCHAPGLE CKIKEHGISG PSEQVTVACE AGWTLTGCNV LPGASLTLGA
660 670 680 690 694
YSVDNLCVAR VHDTARADRT SGEATVAAAI CCRSRPSAKA SWVQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3369 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pcsk9
Peptide Sequence
GTSLHSLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
247
Peptide End
254
CPTAC ID
non-CPTAC-3369
Peptide Molecular Mass
869.4719
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (3+) 15.9 5 6.7 17.4 12.8 7.7 23.6 13.7 10.2 15 15 15
y4 (2+) 4.4 3.9 6.1 15.6 8.1 7.7 16.2 9 9.8 15 15 15
y6 (2+) 4.6 6.2 6.7 14.8 8.8 6.8 15.5 10.8 9.5 15 15 15
y3 (1+) 8.2 4.5 5.8 20.9 10.3 6.8 22.5 11.2 8.9 15 15 15
y4 (1+) 9.9 7.2 5.8 17 11.2 7.5 19.7 13.3 9.5 15 15 15
sum 3.5 3.9 3.9 14.4 8 5.4 14.8 8.9 6.7 15 15 15

Additional Resources and Comments