Apod, CPTAC-3382 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Apod	N/A

Sequence Length (AA)	Molecular Weight (Da)
189	21530

Protein Name
Apolipoprotein D

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVTMLMFLAT	LAGLFTTAKG	QNFHLGKCPS	PPVQENFDVK	KYLGRWYEIE
60	70	80	90	100
KIPASFEKGN	CIQANYSLME	NGNIEVLNKE	LSPDGTMNQV	KGEAKQSNVS
110	120	130	140	150
EPAKLEVQFF	PLMPPAPYWI	LATDYENYAL	VYSCTTFFWL	FHVDFVWILG
160	170	180	189
RNPYLPPETI	TYLKDILTSN	GIDIEKMTTT	DQANCPDFL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3382 Collapse assay details

Data source: Panorama

Official Gene Symbol: Apod
Peptide Sequence: IPASFEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 52
Peptide End: 58

CPTAC ID: non-CPTAC-3382
Peptide Molecular Mass: 790.4225
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (2+)	4.1	2.9	3.2	14.3	8	4.1	14.9	8.5	5.2	15	15	15
y2 (1+)	7.2	4.1	3.2	15.4	8.4	5.8	17	9.3	6.6	15	15	15
y4 (1+)	3.4	3.6	4	12.1	8.8	4.9	12.6	9.5	6.3	15	15	15
y5 (1+)	3.4	3.2	3.5	14.4	9	5.2	14.8	9.6	6.3	15	15	15
y6 (1+)	5.1	4.8	4.8	13	8.2	5.2	14	9.5	7.1	15	15	15
sum	2.8	1.9	2.2	13.8	7.9	3.2	14.1	8.1	3.9	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-1086 Collapse assay details

Data source: Panorama

Official Gene Symbol: APOD
Peptide Sequence: VLNQELR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 76
Peptide End: 82

CPTAC ID: non-CPTAC-1086
Peptide Molecular Mass: 870.4923
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers