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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Egfr N/A
Sequence Length (AA) Molecular Weight (Da)
1210 134853
Protein Name
Epidermal growth factor receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRPSGTARTT LLVLLTALCA AGGALEEKKV CQGTSNRLTQ LGTFEDHFLS
60 70 80 90 100
LQRMYNNCEV VLGNLEITYV QRNYDLSFLK TIQEVAGYVL IALNTVERIP
110 120 130 140 150
LENLQIIRGN ALYENTYALA ILSNYGTNRT GLRELPMRNL QEILIGAVRF
160 170 180 190 200
SNNPILCNMD TIQWRDIVQN VFMSNMSMDL QSHPSSCPKC DPSCPNGSCW
210 220 230 240 250
GGGEENCQKL TKIICAQQCS HRCRGRSPSD CCHNQCAAGC TGPRESDCLV
260 270 280 290 300
CQKFQDEATC KDTCPPLMLY NPTTYQMDVN PEGKYSFGAT CVKKCPRNYV
310 320 330 340 350
VTDHGSCVRA CGPDYYEVEE DGIRKCKKCD GPCRKVCNGI GIGEFKDTLS
360 370 380 390 400
INATNIKHFK YCTAISGDLH ILPVAFKGDS FTRTPPLDPR ELEILKTVKE
410 420 430 440 450
ITGFLLIQAW PDNWTDLHAF ENLEIIRGRT KQHGQFSLAV VGLNITSLGL
460 470 480 490 500
RSLKEISDGD VIISGNRNLC YANTINWKKL FGTPNQKTKI MNNRAEKDCK
510 520 530 540 550
AVNHVCNPLC SSEGCWGPEP RDCVSCQNVS RGRECVEKCN ILEGEPREFV
560 570 580 590 600
ENSECIQCHP ECLPQAMNIT CTGRGPDNCI QCAHYIDGPH CVKTCPAGIM
610 620 630 640 650
GENNTLVWKY ADANNVCHLC HANCTYGCAG PGLQGCEVWP SGPKIPSIAT
660 670 680 690 700
GIVGGLLFIV VVALGIGLFM RRRHIVRKRT LRRLLQEREL VEPLTPSGEA
710 720 730 740 750
PNQAHLRILK ETEFKKIKVL GSGAFGTVYK GLWIPEGEKV KIPVAIKELR
760 770 780 790 800
EATSPKANKE ILDEAYVMAS VDNPHVCRLL GICLTSTVQL ITQLMPYGCL
810 820 830 840 850
LDYVREHKDN IGSQYLLNWC VQIAKGMNYL EDRRLVHRDL AARNVLVKTP
860 870 880 890 900
QHVKITDFGL AKLLGAEEKE YHAEGGKVPI KWMALESILH RIYTHQSDVW
910 920 930 940 950
SYGVTVWELM TFGSKPYDGI PASDISSILE KGERLPQPPI CTIDVYMIMV
960 970 980 990 1000
KCWMIDADSR PKFRELILEF SKMARDPQRY LVIQGDERMH LPSPTDSNFY
1010 1020 1030 1040 1050
RALMDEEDME DVVDADEYLI PQQGFFNSPS TSRTPLLSSL SATSNNSTVA
1060 1070 1080 1090 1100
CINRNGSCRV KEDAFLQRYS SDPTGAVTED NIDDAFLPVP EYVNQSVPKR
1110 1120 1130 1140 1150
PAGSVQNPVY HNQPLHPAPG RDLHYQNPHS NAVGNPEYLN TAQPTCLSSG
1160 1170 1180 1190 1200
FNSPALWIQK GSHQMSLDNP DYQQDFFPKE TKPNGIFKGP TAENAEYLRV
1210
APPSSEFIGA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1781 Collapse assay details

Data source: Panorama

Official Gene Symbol
EGFR
Peptide Modified Sequence
VLGSGAFGTVY[+80.0]K
Modification Type
Phospho (Y)
Protein - Site of Modification
727
Peptide - Site of Modification
11
Peptide Start
717
Peptide End
728
CPTAC ID
CPTAC-1781
Peptide Molecular Mass
1,277.6057
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
E.coli lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 9.1 5.4 5.6 11.8 8.5 6.2 14.9 10.1 8.4 15 15 15
y3 (1+) 42.6 10.9 12.7 37.2 10.6 13.7 56.6 15.2 18.7 15 15 15
y5 (1+) 12.1 6.7 6.8 14.1 7.6 6.6 18.6 10.1 9.5 15 15 15
y6 (1+) 7.7 6 6.9 10.2 7.5 7 12.8 9.6 9.8 15 15 15
y8 (1+) 19.9 8.5 6.1 20.6 9 10.2 28.6 12.4 11.9 15 15 15
y9 (1+) 19.5 10.2 10.4 31 9.5 9.7 36.6 13.9 14.2 15 15 15
y10 (1+) 9.5 5.5 6.4 8.4 7.2 6.1 12.7 9.1 8.8 15 15 15
sum 7.6 4 5.7 6.9 5.1 6 10.3 6.5 8.3 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3383 Collapse assay details

Data source: Panorama

Official Gene Symbol
Egfr
Peptide Sequence
IPLENLQIIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
99
Peptide End
108
CPTAC ID
non-CPTAC-3383
Peptide Molecular Mass
1,207.7289
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 4.7 4.4 3.8 11 6.9 4.8 12 8.2 6.1 15 15 15
y9 (2+) 4.7 5.5 2.6 15.1 9.5 3.9 15.8 11 4.7 15 15 15
y6 (1+) 2.5 3.8 3.1 13 6.5 4.6 13.2 7.5 5.5 15 15 15
y7 (1+) 3.8 3.4 4.7 16.6 6.3 4.7 17 7.2 6.6 15 15 15
y8 (1+) 3.4 3.2 2.8 13.6 10.6 6 14 11.1 6.6 15 15 15
sum 2.5 2.7 1.7 14.1 8 2.9 14.3 8.4 3.4 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-802 Collapse assay details

Data source: Panorama

Official Gene Symbol
EGFR
Peptide Modified Sequence
GSHQISLDNPDY[+80.0]QQDFFPK
Modification Type
Phospho (Y)
Protein - Site of Modification
1172
Peptide - Site of Modification
12
Peptide Start
1161
Peptide End
1179
CPTAC ID
CPTAC-802
Peptide Molecular Mass
2,314.9896
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 35.3 17.3 12.2 28.3 18.2 12.9 45.2 25.1 17.8 15 15 15
y5 (1+) 28.5 18.3 17.1 31.4 21.9 27 42.4 28.5 32 15 15 15
y6 (1+) 39.5 27.2 21.2 48 34.6 21.5 62.2 44 30.2 15 15 15
b8 (1+) 37.4 22.2 17.4 40.5 22.2 20.5 55.1 31.4 26.9 15 15 15
b6 (1+) 40.5 16.6 13.2 45.5 21.9 21.3 60.9 27.5 25.1 15 15 15
b5 (1+) 23.2 11.6 18.3 24.2 15.9 15.9 33.5 19.7 24.2 15 15 15
b4 (1+) 28.5 8.3 11 31.7 9.2 11.3 42.6 12.4 15.8 15 15 15
b3 (1+) 35.8 12.9 7.5 37 13.9 9.5 51.5 19 12.1 15 15 15
sum 11.9 3.7 6.3 12.4 6.1 5.4 17.2 7.1 8.3 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1043 Collapse assay details

Data source: Panorama

Official Gene Symbol
EGFR
Peptide Modified Sequence
MHLPSPTDSNFY[+80.0]R
Modification Type
Phospho (Y)
Protein - Site of Modification
998
Peptide - Site of Modification
12
Peptide Start
987
Peptide End
999
CPTAC ID
CPTAC-1043
Peptide Molecular Mass
1,643.6803
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 24.7 15.9 9.1 29.1 13.3 8 38.2 20.7 12.1 15 15 15
y3 (1+) 23.3 14.6 10.1 27.6 13 10.7 36.1 19.5 14.7 15 15 15
b3 (1+) 18.5 10.3 5.9 17.4 7.5 7.6 25.4 12.7 9.6 15 15 15
sum 11.1 4.7 4.9 10.3 5 6.2 15.1 6.9 7.9 15 15 15

Additional Resources and Comments