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Overview Data source: UniProt

Official Gene Symbol Other Aliases
EGFR ERBB, ERBB1, HER1
Sequence Length (AA) Molecular Weight (Da)
1210 134277
Protein Name
Epidermal growth factor receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRPSGTAGAA LLALLAALCP ASRALEEKKV CQGTSNKLTQ LGTFEDHFLS
60 70 80 90 100
LQRMFNNCEV VLGNLEITYV QRNYDLSFLK TIQEVAGYVL IALNTVERIP
110 120 130 140 150
LENLQIIRGN MYYENSYALA VLSNYDANKT GLKELPMRNL QEILHGAVRF
160 170 180 190 200
SNNPALCNVE SIQWRDIVSS DFLSNMSMDF QNHLGSCQKC DPSCPNGSCW
210 220 230 240 250
GAGEENCQKL TKIICAQQCS GRCRGKSPSD CCHNQCAAGC TGPRESDCLV
260 270 280 290 300
CRKFRDEATC KDTCPPLMLY NPTTYQMDVN PEGKYSFGAT CVKKCPRNYV
310 320 330 340 350
VTDHGSCVRA CGADSYEMEE DGVRKCKKCE GPCRKVCNGI GIGEFKDSLS
360 370 380 390 400
INATNIKHFK NCTSISGDLH ILPVAFRGDS FTHTPPLDPQ ELDILKTVKE
410 420 430 440 450
ITGFLLIQAW PENRTDLHAF ENLEIIRGRT KQHGQFSLAV VSLNITSLGL
460 470 480 490 500
RSLKEISDGD VIISGNKNLC YANTINWKKL FGTSGQKTKI ISNRGENSCK
510 520 530 540 550
ATGQVCHALC SPEGCWGPEP RDCVSCRNVS RGRECVDKCN LLEGEPREFV
560 570 580 590 600
ENSECIQCHP ECLPQAMNIT CTGRGPDNCI QCAHYIDGPH CVKTCPAGVM
610 620 630 640 650
GENNTLVWKY ADAGHVCHLC HPNCTYGCTG PGLEGCPTNG PKIPSIATGM
660 670 680 690 700
VGALLLLLVV ALGIGLFMRR RHIVRKRTLR RLLQERELVE PLTPSGEAPN
710 720 730 740 750
QALLRILKET EFKKIKVLGS GAFGTVYKGL WIPEGEKVKI PVAIKELREA
760 770 780 790 800
TSPKANKEIL DEAYVMASVD NPHVCRLLGI CLTSTVQLIT QLMPFGCLLD
810 820 830 840 850
YVREHKDNIG SQYLLNWCVQ IAKGMNYLED RRLVHRDLAA RNVLVKTPQH
860 870 880 890 900
VKITDFGLAK LLGAEEKEYH AEGGKVPIKW MALESILHRI YTHQSDVWSY
910 920 930 940 950
GVTVWELMTF GSKPYDGIPA SEISSILEKG ERLPQPPICT IDVYMIMVKC
960 970 980 990 1000
WMIDADSRPK FRELIIEFSK MARDPQRYLV IQGDERMHLP SPTDSNFYRA
1010 1020 1030 1040 1050
LMDEEDMDDV VDADEYLIPQ QGFFSSPSTS RTPLLSSLSA TSNNSTVACI
1060 1070 1080 1090 1100
DRNGLQSCPI KEDSFLQRYS SDPTGALTED SIDDTFLPVP EYINQSVPKR
1110 1120 1130 1140 1150
PAGSVQNPVY HNQPLNPAPS RDPHYQDPHS TAVGNPEYLN TVQPTCVNST
1160 1170 1180 1190 1200
FDSPAHWAQK GSHQISLDNP DYQQDFFPKE AKPNGIFKGS TAENAEYLRV
1210
APQSSEFIGA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1781 Collapse assay details

Data source: Panorama

Official Gene Symbol
EGFR
Peptide Modified Sequence
VLGSGAFGTVY[+80.0]K
Modification Type
Phospho (Y)
Protein - Site of Modification
727
Peptide - Site of Modification
11
Peptide Start
717
Peptide End
728
CPTAC ID
CPTAC-1781
Peptide Molecular Mass
1,277.6057
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 9.1 5.4 5.6 11.8 8.5 6.2 14.9 10.1 8.4 15 15 15
y3 (1+) 42.6 10.9 12.7 37.2 10.6 13.7 56.6 15.2 18.7 15 15 15
y5 (1+) 12.1 6.7 6.8 14.1 7.6 6.6 18.6 10.1 9.5 15 15 15
y6 (1+) 7.7 6 6.9 10.2 7.5 7 12.8 9.6 9.8 15 15 15
y8 (1+) 19.9 8.5 6.1 20.6 9 10.2 28.6 12.4 11.9 15 15 15
y9 (1+) 19.5 10.2 10.4 31 9.5 9.7 36.6 13.9 14.2 15 15 15
y10 (1+) 9.5 5.5 6.4 8.4 7.2 6.1 12.7 9.1 8.8 15 15 15
sum 7.6 4 5.7 6.9 5.1 6 10.3 6.5 8.3 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3383 Collapse assay details

Data source: Panorama

Official Gene Symbol
Egfr
Peptide Sequence
IPLENLQIIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
99
Peptide End
108
CPTAC ID
non-CPTAC-3383
Peptide Molecular Mass
1,207.7289
Species
Mus musculus (Mouse)
Assay Type
Direct PRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 4.7 4.4 3.8 11 6.9 4.8 12 8.2 6.1 15 15 15
y9 (2+) 4.7 5.5 2.6 15.1 9.5 3.9 15.8 11 4.7 15 15 15
y6 (1+) 2.5 3.8 3.1 13 6.5 4.6 13.2 7.5 5.5 15 15 15
y7 (1+) 3.8 3.4 4.7 16.6 6.3 4.7 17 7.2 6.6 15 15 15
y8 (1+) 3.4 3.2 2.8 13.6 10.6 6 14 11.1 6.6 15 15 15
sum 2.5 2.7 1.7 14.1 8 2.9 14.3 8.4 3.4 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-802 Collapse assay details

Data source: Panorama

Official Gene Symbol
EGFR
Peptide Modified Sequence
GSHQISLDNPDY[+80.0]QQDFFPK
Modification Type
Phospho (Y)
Protein - Site of Modification
1172
Peptide - Site of Modification
12
Peptide Start
1161
Peptide End
1179
CPTAC ID
CPTAC-802
Peptide Molecular Mass
2,314.9896
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 35.3 17.3 12.2 28.3 18.2 12.9 45.2 25.1 17.8 15 15 15
y5 (1+) 28.5 18.3 17.1 31.4 21.9 27 42.4 28.5 32 15 15 15
y6 (1+) 39.5 27.2 21.2 48 34.6 21.5 62.2 44 30.2 15 15 15
b8 (1+) 37.4 22.2 17.4 40.5 22.2 20.5 55.1 31.4 26.9 15 15 15
b6 (1+) 40.5 16.6 13.2 45.5 21.9 21.3 60.9 27.5 25.1 15 15 15
b5 (1+) 23.2 11.6 18.3 24.2 15.9 15.9 33.5 19.7 24.2 15 15 15
b4 (1+) 28.5 8.3 11 31.7 9.2 11.3 42.6 12.4 15.8 15 15 15
b3 (1+) 35.8 12.9 7.5 37 13.9 9.5 51.5 19 12.1 15 15 15
sum 11.9 3.7 6.3 12.4 6.1 5.4 17.2 7.1 8.3 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1043 Collapse assay details

Data source: Panorama

Official Gene Symbol
EGFR
Peptide Modified Sequence
MHLPSPTDSNFY[+80.0]R
Modification Type
Phospho (Y)
Protein - Site of Modification
998
Peptide - Site of Modification
12
Peptide Start
987
Peptide End
999
CPTAC ID
CPTAC-1043
Peptide Molecular Mass
1,643.6803
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 24.7 15.9 9.1 29.1 13.3 8 38.2 20.7 12.1 15 15 15
y3 (1+) 23.3 14.6 10.1 27.6 13 10.7 36.1 19.5 14.7 15 15 15
b3 (1+) 18.5 10.3 5.9 17.4 7.5 7.6 25.4 12.7 9.6 15 15 15
sum 11.1 4.7 4.9 10.3 5 6.2 15.1 6.9 7.9 15 15 15

Additional Resources and Comments