F10, CPTAC-3413 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
F10	N/A

Sequence Length (AA)	Molecular Weight (Da)
481	54018

Protein Name
Coagulation factor X

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGSPVQLSLL	CVVLASLLLP	GKGVFINRER	ANNVLARTRR	ANSFFEEFKK
60	70	80	90	100
GNLERECMEE	ICSYEEVREI	FEDDEKTKEY	WTKYKDGDQC	ESSPCQNQGA
110	120	130	140	150
CRDGIGGYTC	TCSEGFEGKN	CELFVRKLCR	LDNGDCDQFC	REEQNSVVCS
160	170	180	190	200
CASGYFLGND	GKSCISTAPF	PCGKITTGRR	KRSVALNTSD	SELDLEDALL
210	220	230	240	250
DEDFLSPTEN	PIELLNLNET	QPERSSDDLV	RIVGGRECKD	GECPWQALLI
260	270	280	290	300
NEDNEGFCGG	TILNEFYILT	AAHCLHQARR	FKVRVGDRNT	EKEEGNEMVH
310	320	330	340	350
EVDVVIKHNK	FQRDTYDYDI	AVLRLKTPIT	FRMNVAPACL	PQKDWAESTL
360	370	380	390	400
MTQKTGIVSG	FGRTHEKGRQ	SNILKMLEVP	YVDRNTCKLS	TSFSITQNMF
410	420	430	440	450
CAGYEAKLED	ACQGDSGGPH	VTRFKNTYYV	TGIVSWGEGC	ARKGKYGIYT
460	470	480	481
KVTTFLKWID	RSMKARVGPT	AETPRTAGPP	N

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2649 Collapse assay details

Data source: Panorama

Official Gene Symbol: F10
Peptide Sequence: MLEVPYVDR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 379
Peptide End: 387

CPTAC ID: non-CPTAC-2649
Peptide Molecular Mass: 1,120.5587
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	12.5	7.3	6.1	13.2	7.6	7.4	18.2	10.5	9.6	15	15	15
y7 (1+)	8.4	7.4	4.3	10.1	9.2	6.4	13.1	11.8	7.7	15	15	15
b3 (1+)	10.3	4.4	6.2	16.2	6.4	8.9	19.2	7.8	10.8	15	15	15
y5 (1+)	5.5	3.4	4.9	12.6	7.3	6.9	13.7	8.1	8.5	15	15	15
b4 (1+)	8.4	6.4	2.9	10.2	9.8	6.9	13.2	11.7	7.5	15	15	15
sum	4.4	3.1	3.4	9.9	6.8	5.8	10.8	7.5	6.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3413 Collapse assay details

Data source: Panorama

Official Gene Symbol: F10
Peptide Sequence: TGIVSGFGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 355
Peptide End: 363

CPTAC ID: non-CPTAC-3413
Peptide Molecular Mass: 892.4767
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.