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Overview Data source: UniProt

Official Gene Symbol Other Aliases
F10 N/A
Sequence Length (AA) Molecular Weight (Da)
488 54732
Protein Name
Coagulation factor X
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGRPLHLVLL SASLAGLLLL GESLFIRREQ ANNILARVTR ANSFLEEMKK
60 70 80 90 100
GHLERECMEE TCSYEEAREV FEDSDKTNEF WNKYKDGDQC ETSPCQNQGK
110 120 130 140 150
CKDGLGEYTC TCLEGFEGKN CELFTRKLCS LDNGDCDQFC HEEQNSVVCS
160 170 180 190 200
CARGYTLADN GKACIPTGPY PCGKQTLERR KRSVAQATSS SGEAPDSITW
210 220 230 240 250
KPYDAADLDP TENPFDLLDF NQTQPERGDN NLTRIVGGQE CKDGECPWQA
260 270 280 290 300
LLINEENEGF CGGTILSEFY ILTAAHCLYQ AKRFKVRVGD RNTEQEEGGE
310 320 330 340 350
AVHEVEVVIK HNRFTKETYD FDIAVLRLKT PITFRMNVAP ACLPERDWAE
360 370 380 390 400
STLMTQKTGI VSGFGRTHEK GRQSTRLKML EVPYVDRNSC KLSSSFIITQ
410 420 430 440 450
NMFCAGYDTK QEDACQGDSG GPHVTRFKDT YFVTGIVSWG EGCARKGKYG
460 470 480 488
IYTKVTAFLK WIDRSMKTRG LPKAKSHAPE VITSSPLK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-2649 Collapse assay details

Data source: Panorama

Official Gene Symbol
F10
Peptide Sequence
MLEVPYVDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
379
Peptide End
387
CPTAC ID
non-CPTAC-2649
Peptide Molecular Mass
1,120.5587
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 12.5 7.3 6.1 13.2 7.6 7.4 18.2 10.5 9.6 15 15 15
y7 (1+) 8.4 7.4 4.3 10.1 9.2 6.4 13.1 11.8 7.7 15 15 15
b3 (1+) 10.3 4.4 6.2 16.2 6.4 8.9 19.2 7.8 10.8 15 15 15
y5 (1+) 5.5 3.4 4.9 12.6 7.3 6.9 13.7 8.1 8.5 15 15 15
b4 (1+) 8.4 6.4 2.9 10.2 9.8 6.9 13.2 11.7 7.5 15 15 15
sum 4.4 3.1 3.4 9.9 6.8 5.8 10.8 7.5 6.7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3413 Collapse assay details

Data source: Panorama

Official Gene Symbol
F10
Peptide Sequence
TGIVSGFGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
355
Peptide End
363
CPTAC ID
non-CPTAC-3413
Peptide Molecular Mass
892.4767
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 7.9 5 4.6 13.5 7.8 4.6 15.6 9.3 6.5 15 15 15
b3 (1+) 5.4 5.8 4.2 13.2 7.5 7.8 14.3 9.5 8.9 15 15 15
y4 (1+) 9.1 6.6 5 18.8 9.3 6.1 20.9 11.4 7.9 15 15 15
y5 (1+) 5.1 4.8 4 13 7.1 7.6 14 8.6 8.6 15 15 15
y6 (1+) 5.9 4.4 8.1 20.6 12.1 7.2 21.4 12.9 10.8 15 15 15
sum 1.8 2.1 3.3 14.6 8 4.5 14.7 8.3 5.6 15 15 15

Additional Resources and Comments