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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
F11 N/A
Sequence Length (AA) Molecular Weight (Da)
624 69789
Protein Name
Coagulation factor XI
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTSLHQVLYF IFFASVSSEC VTKVFKDISF QGGDLSTVFT PSATYCRLVC
60 70 80 90 100
THHPRCLLFT FMAESSSDDP TKWFACILKD SVTEILPMVN MTGAISGYSF
110 120 130 140 150
KQCPQQLSTC SKDVYVNLDM KGMNYNSSVV KNARECQERC TDDAHCQFFT
160 170 180 190 200
YATGYFPSVD HRKMCLLKYT RTGTPTTITK LNGVVSGFSL KSCGLSNLAC
210 220 230 240 250
IRDIFPNTVL ADLNIDSVVA PDAFVCRRIC THHPTCLFFT FFSQAWPKES
260 270 280 290 300
QRHLCLLKTS ESGLPSTRIT KSHALSGFSL QHCRHSVPVF CHPSFYNDTD
310 320 330 340 350
FLGEELDIVD VKGQETCQKT CTNNARCQFF TYYPSHRLCN ERNRRGRCYL
360 370 380 390 400
KLSSNGSPTR ILHGRGGISG YSLRLCKMDN VCTTKINPRV VGGAASVHGE
410 420 430 440 450
WPWQVTLHIS QGHLCGGSII GNQWILTAAH CFSGIETPKK LRVYGGIVNQ
460 470 480 490 500
SEINEGTAFF RVQEMIIHDQ YTTAESGYDI ALLKLESAMN YTDFQRPICL
510 520 530 540 550
PSKGDRNAVH TECWVTGWGY TALRGEVQST LQKAKVPLVS NEECQTRYRR
560 570 580 590 600
HKITNKMICA GYKEGGKDTC KGDSGGPLSC KYNGVWHLVG ITSWGEGCGQ
610 620 624
KERPGVYTNV AKYVDWILEK TQTV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3420 Collapse assay details

Data source: Panorama

Official Gene Symbol
F11
Peptide Sequence
TSESGLPSTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
259
Peptide End
268
CPTAC ID
non-CPTAC-3420
Peptide Molecular Mass
1,033.5040
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 7.8 9.7 4.9 17.4 10.9 5.3 19.1 14.6 7.2 15 15 15
y7 (1+) 11.7 6.6 9.1 51.9 19.3 11 53.2 20.4 14.3 15 15 15
y6 (1+) 5 5.4 8 16 7.2 10.1 16.8 9 12.9 15 15 15
y4 (1+) 7.4 8.5 5.7 15.2 10.7 4.7 16.9 13.7 7.4 15 15 15
y8 (2+) 10.1 5.8 7.2 18.2 11.4 7.2 20.8 12.8 10.2 15 15 15
sum 6.5 4.2 3.5 28.9 8.2 3.5 29.6 9.2 4.9 15 15 15


Additional Resources and Comments