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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Acsl3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Acsl3 Acs3, Facl3
Sequence Length (AA) Molecular Weight (Da)
720 80492
Protein Name
Long-chain-fatty-acid--CoA ligase 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MNNHVSSTPS TMKLKQTINP ILLYFIHFII SLYTILTYIP FYFLCESKQE
60 70 80 90 100
KPNQIKAKPV SSKPDSAYRS INSVDGLASV LYPGCDTLDK VFMYAKNKFK
110 120 130 140 150
NKRLLGTREI LNEEDEIQPN GKIFKKVILG HYNWLSYEDV FIRALDFGNG
160 170 180 190 200
LQMLGQKPKA NIAIFCETRA EWMIAAQACF MYNFQLVTLY ATLGGPAIVH
210 220 230 240 250
GLNETEVTNI ITSKELLQTK LKDIVSLVPR LRHIITVDGK PPTWSEFPKG
260 270 280 290 300
VIVHTMAAVQ ALGVKANVEK KAHSKPLPSD IAVIMYTSGS TGIPKGVMIS
310 320 330 340 350
HSNIIASITG MARRIPRLGE EDVYIGYLPL AHVLELSAEL VCLSHGCRIG
360 370 380 390 400
YSSPQTLADQ SSKIKKGSKG DTSVLKPTLM AAVPEIMDRI YKNVMNKVNE
410 420 430 440 450
MSAFQRNLFI LAYNYKMEQI SKGCSTPLCD RFVFRNVRRL LGGNIRLLLC
460 470 480 490 500
GGAPLSATTQ RFMNICFCCP VGQGYGLTES TGAGTITEVW DYNTGRVGAP
510 520 530 540 550
LVCCEIKLKN WEEGGYFNTD KPHPRGEILI GGQNVTMGYY KNEAKTKTDF
560 570 580 590 600
FEDENGQRWL CTGDIGEFDP DGCLKIIDRK KDLVKLQAGE YVSLGKVEAA
610 620 630 640 650
LKNLPLIDNI CAYANSYHSY VIGFVVPNQK ELTELARTKG FKGTWEELCN
660 670 680 690 700
SSEMENEVLK VLSEAAISAS LEKFEIPLKI RLSPDPWTPE TGLVTDAFKL
710 720
KRKELKTHYQ ADIERMYGRK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3442 Collapse assay details

Data source: Panorama

Official Gene Symbol
Acsl3
Peptide Sequence
VLSEAAISASLEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
661
Peptide End
673
CPTAC ID
non-CPTAC-3442
Peptide Molecular Mass
1,316.7187
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.7 1.8 2.5 10.8 5.2 3.6 12.2 5.5 4.4 15 15 15
y6 (1+) 11.5 2.8 2.5 10.7 3.9 5.4 15.7 4.8 6 15 15 15
y7 (1+) 9.6 4.5 3.5 9.6 5.7 4.6 13.6 7.3 5.8 15 15 15
y8 (1+) 8.2 4.6 4.2 13.5 6 4.4 15.8 7.6 6.1 15 15 15
y11 (1+) 5.4 4.2 3.2 10.3 5.1 5.5 11.6 6.6 6.4 15 15 15
sum 5 2.1 1.1 9.4 4 3.8 10.6 4.5 4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments