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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Snap91

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Snap91 N/A
Sequence Length (AA) Molecular Weight (Da)
901 91851
Protein Name
Clathrin coat assembly protein AP180
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSGQTLTDRI AAAQYSVTGS AVARAVCKAT THEVMGPKKK HLDYLIQATN
60 70 80 90 100
ETNVNIPQMA DTLFERATNS SWVVVFKALV TTHHLMVHGN ERFIQYLASR
110 120 130 140 150
NTLFNLSNFL DKSGSHGYDM STFIRRYSRY LNEKAFSYRQ MAFDFARVKK
160 170 180 190 200
GADGVMRTMV PEKLLKSMPI LQGQIDALLE FDVHPNELTN GVINAAFMLL
210 220 230 240 250
FKDLIKLFAC YNDGVINLLE KFFEMKKGQC KDALEIYKRF LTRMTRVSEF
260 270 280 290 300
LKVAEQVGID KGDIPDLTQA PSSLMETLEQ HLNTLEGKKP GNNEGSGAPS
310 320 330 340 350
PLSKSSPATT VTSPNSTPAK TIDTSPPVDI FATASAAAPV SSAKPSSDLL
360 370 380 390 400
DLQPDFSGAA AGAAAPVVPP SGGATAWGDL LGEDSLAALS SVPCEAPISD
410 420 430 440 450
PFAPEPSPPT TTTEPASASA STTTAVTAVT TEVDLFGDAF AASPGEAPAA
460 470 480 490 500
SEGATAPATP APVAAALDAC SGNDPFAPSE GSAEAAPELD LFAMKPPETS
510 520 530 540 550
APVVTPTAST APPVPATAPS PAPTAVAATA ATTTAAAAAT TTATTSAAAA
560 570 580 590 600
TTAAAPPALD IFGDLFDSAP EVAAAPKPDA APSIDLFGTD AFSSPPRGAS
610 620 630 640 650
PVPESSLTAD LLSVDAFAAP SPASTASPAK AESSGVIDLF GDAFGSGASE
660 670 680 690 700
TQPAPQAVSS SSASADLLAG FGGSFMAPST TPVTPAQNNL LQPSFEAAFG
710 720 730 740 750
TTPSTSSSSS FDPSVFDGLG DLLMPTMAPS GQPAPVSMVP PSPAMAASKG
760 770 780 790 800
LGSDLDSSLA SLVGNLGISG TTSKKGDLQW NAGEKKLTGG ANWQPKVTPA
810 820 830 840 850
TWSAGVPPQG TVPPTSSVPP GAGAPSVGQP GAGFGMPPSG TGMTMMSQQP
860 870 880 890 900
VMFAQPMMRP PFGAAAVPGT QLSPSPTPAT QSPKKPPAKD PLADLNIKDF
901
L

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3444 Collapse assay details

Data source: Panorama

Official Gene Symbol
Snap91
Peptide Sequence
IAAAQYSVTGSAVAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
10
Peptide End
24
CPTAC ID
non-CPTAC-3444
Peptide Molecular Mass
1,463.7732
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 29.5 2.2 5.9 29.5 7.3 7.9 41.7 7.6 9.9 15 15 15
y7 (1+) 13.6 6.4 4.2 16.7 5.8 5.4 21.5 8.6 6.8 15 15 15
y8 (1+) 19.3 7.4 3.6 21.3 8.9 9 28.7 11.6 9.7 15 15 15
y9 (1+) 14.8 7.2 7.3 14.7 8 8.5 20.9 10.8 11.2 15 15 15
y10 (1+) 6.9 3.2 3.7 7.2 7.4 7.3 10 8.1 8.2 15 15 15
sum 6.9 2.8 2.2 6.6 4.8 4.9 9.5 5.6 5.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments