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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Pam N/A
Sequence Length (AA) Molecular Weight (Da)
979 108963
Protein Name
Peptidyl-glycine alpha-amidating monooxygenase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGRARSRLL LLLGLLALQS SCLAFRSPLS VFKRFKETTR SFSNECLGTT
60 70 80 90 100
RPITPIDSSD FTLDIRMPGV TPKESDTYFC MSMRLPVDEE AFVIDFKPRA
110 120 130 140 150
SMDTVHHMLL FGCNMPSSTG SYWFCDEGTC TDKANILYAW ARNAPPTRLP
160 170 180 190 200
KGVGFRVGGE TGSKYFVLQV HYGDISAFRD NHKDCSGVSL HLTRVPQPLI
210 220 230 240 250
AGMYLMMSVN TVIPPGEKVV NSDISCHYKM YPMHVFAYRV HTHHLGKVVS
260 270 280 290 300
GYRVRNGQWT LIGRQSPQLP QAFYPVEHPV DVAFGDILAA RCVFTGEGRT
310 320 330 340 350
EATHIGGTSS DEMCNLYIMY YMEAKHAVSF MTCTQNVAPD MFRTIPEEAN
360 370 380 390 400
IPIPVKSDMV MIHGHHKETE NKEKSALIQQ PKQGEEEAFE QGDFYSLLSK
410 420 430 440 450
LLGEREDVVH VHKYNPTEKT ESGSDLVAEI ANVVQKKDLG RSDAREGAEH
460 470 480 490 500
EEGGNAILVR DRIHKFHRLE STLRPAESRA LSFQQPGEGP WEPELAGDFH
510 520 530 540 550
VEEALEWPGV YLLPGQVSGV ALDSKNNLVI FHRGDHVWDG NSFDSKFVYQ
560 570 580 590 600
QRGLGPIEED TILVIDPNKA EILQSSGKNL FYLPHGLSID TDGNYWVTDV
610 620 630 640 650
ALHQVFKLEP RSKEGPLLVL GRSMQPGSDQ NHFCQPTDVA VEPSTGAVFV
660 670 680 690 700
SDGYCNSRIV QFSPSGKFIT QWGEESSGSS PKPGQFSVPH SLALVPHLNQ
710 720 730 740 750
LCVADRENGR IQCFKTDTKE FVREIKHASF GRNVFAISYI PGFLFAVNGK
760 770 780 790 800
PYFGDQEPVQ GFVMNFSSGE IIDVFKPVRK HFDMPHDIVA SEDGTVYIGD
810 820 830 840 850
AHTNTVWKFT LTESRLEVEH RSVKKAGIEV PEIKEAEAVV EPKVKNKPTS
860 870 880 890 900
SELQKMQEKK KLIKDPGSGV PVVLITTLLV IPVVVLLAIA MFIRWKKSRA
910 920 930 940 950
FGDHDRKLES SSGRVLGRLR GKGSSGLNLG NFFASRKGYS RKGFDRVSTE
960 970 979
GSDQEKDEDD GSESEEEYSA PLPTPAPSS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3445 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pam
Peptide Sequence
EGPLLVLGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
614
Peptide End
622
CPTAC ID
non-CPTAC-3445
Peptide Molecular Mass
952.5706
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 33.8 11 10.8 36.3 15 11 49.6 18.6 15.4 15 15 15
y5 (1+) 13.3 5.8 4.9 15.7 6.9 5.5 20.6 9 7.4 15 15 15
y4 (1+) 8.7 5.5 5.2 10.4 5.7 6.1 13.6 7.9 8 15 15 15
y3 (1+) 6.5 7.8 4.9 8.8 6.8 6.2 10.9 10.3 7.9 15 15 15
y2 (1+) 9.2 5.5 5 10.1 6.9 6.1 13.7 8.8 7.9 15 15 15
sum 5.2 3 2.3 7.1 3.4 3.7 8.8 4.5 4.4 15 15 15

Additional Resources and Comments