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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Aebp1 Aclp
Sequence Length (AA) Molecular Weight (Da)
1128 128365
Protein Name
Adipocyte enhancer-binding protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPVRTASLL CGLLALLTLC PEGNPQTVLT DDEIEEFLEG FLSELETQSP
60 70 80 90 100
PREDDVEVQP LPEPTQRPRK SKAGGKQRAD VEVPPEKNKD KEKKGKKDKG
110 120 130 140 150
PKATKPLEGS TRPTKKPKEK PPKATKKPKE KPPKATKKPK EKPPKATKKP
160 170 180 190 200
KEKPPKATKR PSAGKKFSTV APLETLDRLL PSPSNPSAQE LPQKRDTPFP
210 220 230 240 250
NAWQGQGEET QVEAKQPRPE PEEETEMPTL DYNDQIEKED YEDFEYIRRQ
260 270 280 290 300
KQPRPTPSRR RLWPERPEEK TEEPEERKEV EPPLKPLLPP DYGDSYVIPN
310 320 330 340 350
YDDLDYYFPH PPPQKPDVGQ EVDEEKEEMK KPKKEGSSPK EDTEDKWTVE
360 370 380 390 400
KNKDHKGPRK GEELEEEWAP VEKIKCPPIG MESHRIEDNQ IRASSMLRHG
410 420 430 440 450
LGAQRGRLNM QAGANEDDYY DGAWCAEDES QTQWIEVDTR RTTRFTGVIT
460 470 480 490 500
QGRDSSIHDD FVTTFFVGFS NDSQTWVMYT NGYEEMTFYG NVDKDTPVLS
510 520 530 540 550
ELPEPVVARF IRIYPLTWNG SLCMRLEVLG CPVTPVYSYY AQNEVVTTDS
560 570 580 590 600
LDFRHHSYKD MRQLMKAVNE ECPTITRTYS LGKSSRGLKI YAMEISDNPG
610 620 630 640 650
DHELGEPEFR YTAGIHGNEV LGRELLLLLM QYLCQEYRDG NPRVRNLVQD
660 670 680 690 700
TRIHLVPSLN PDGYEVAAQM GSEFGNWALG LWTEEGFDIF EDFPDLNSVL
710 720 730 740 750
WAAEEKKWVP YRVPNNNLPI PERYLSPDAT VSTEVRAIIS WMEKNPFVLG
760 770 780 790 800
ANLNGGERLV SYPYDMARTP SQEQLLAEAL AAARGEDDDG VSEAQETPDH
810 820 830 840 850
AIFRWLAISF ASAHLTMTEP YRGGCQAQDY TSGMGIVNGA KWNPRSGTFN
860 870 880 890 900
DFSYLHTNCL ELSVYLGCDK FPHESELPRE WENNKEALLT FMEQVHRGIK
910 920 930 940 950
GVVTDEQGIP IANATISVSG INHGVKTASG GDYWRILNPG EYRVTAHAEG
960 970 980 990 1000
YTSSAKICNV DYDIGATQCN FILARSNWKR IREILAMNGN RPILRVDPSR
1010 1020 1030 1040 1050
PMTPQQRRMQ QRRLQYRLRM REQMRLRRLN STAGPATSPT PALMPPPSPT
1060 1070 1080 1090 1100
PAITLRPWEV LPTTTAGWEE SETETYTEVV TEFETEYGTD LEVEEIEEEE
1110 1120 1128
EEEEEEMDTG LTFPLTTVET YTVNFGDF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3446 Collapse assay details

Data source: Panorama

Official Gene Symbol
Aebp1
Peptide Sequence
YTAGIHGNEVLGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
611
Peptide End
623
CPTAC ID
non-CPTAC-3446
Peptide Molecular Mass
1,385.7052
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 9.6 6.7 6.2 12.6 9 6.9 15.8 11.2 9.3 15 15 15
y12 (2+) 8 5.4 2.7 11.4 6.2 3.2 13.9 8.2 4.2 15 15 15
y11 (2+) 6.1 5.2 4.8 8.5 5.6 4.5 10.5 7.6 6.6 15 15 15
y8 (2+) 15 5.1 4.2 15.9 7.7 5.5 21.9 9.2 6.9 15 15 15
b2 (1+) 9.4 4.9 2.9 9.3 5.3 4.4 13.2 7.2 5.3 15 15 15
sum 4.1 3.5 2 7.5 4.9 2.7 8.5 6 3.4 15 15 15


Additional Resources and Comments