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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Apobr Apob48r
Sequence Length (AA) Molecular Weight (Da)
942 102705
Protein Name
Apolipoprotein B receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDFLRLRLPG LHQALRGALD SFSAFVSYLV GDTVPTVERQ TQAAEELGEV
60 70 80 90 100
TEGKVVGEEA QEVLEGLRSG QSEGVEAPEE TRRCQEGSLA GEQTWGWRAD
110 120 130 140 150
SSARPQAERQ DTGSWKAAED ARGQEPSVPL KPEAEPGTHR DRSSNTAQEI
160 170 180 190 200
WEHGEEEASS GEPLRTCEQK EEEEEVVRAA ESGMAEGVES QPTWHSEPGG
210 220 230 240 250
NAGTEGQHVT EDSKEIDWVA KDMVAEIEWF GAKGIDKEEE RMVPMRDGER
260 270 280 290 300
ARAQGTQCPG AESEDQAMLS REAWTVSDRE GADSLGVQGT EYGSDPGDNF
310 320 330 340 350
PGTTGRVWVL EEADKGDQQD EVDEKREAEV RFPIQTLEAE RTGEMTEGHI
360 370 380 390 400
AEEEAMGEQE TEGSFEDEER QDLAIRDNGV SLEEEVRAEE SSREKRNSWA
410 420 430 440 450
TEPTLVLDTE AKDEPDWEDS PEVSTEELFV GERSEAAQMT PEVLRVKVTE
460 470 480 490 500
GQDPELVRHS QALTKQLEEG QKGQEETSGA PDLSPERVLS LKEYPGPVGF
510 520 530 540 550
AGPELEAWGN WSRGVDRRNS QEVKADAEAG KEQTATEQAV EIRAEGGQEA
560 570 580 590 600
QQPEVFGSGG EEALTSVALN PELEGSQGAE AGTEESVEES KPTENEAAEE
610 620 630 640 650
EAVVPWEADG TCRKRRLEEV TLSLQDSEDT ETSYLAEEII VGIRAVDTEE
660 670 680 690 700
GPKWEAGLAP ETELGKAWCS EGRGEAGRGT ELEETTEKQS GQEVGLVGSA
710 720 730 740 750
EKVSGYDIQE IDGTEEGEQA EMETSVMAED IRGTDGVTLG SQAERAEGSI
760 770 780 790 800
TPMETEGLLR DQMLLEEEAG GGQSREQKVH NSEGEIQTLD DSSDQEGQQT
810 820 830 840 850
HQIPTVAVPG PLESAEATAG APGDVHSNWN EALLPGSRLD VSVPRSRVLL
860 870 880 890 900
SRSSSRRRSR PSFHRISVPE PQCDPPSPQP QAERPVPEQS SLQLEETPEL
910 920 930 940 942
SATKPEGTPV PARRKMLGRG FGFAHPGMMQ ELQARLSQPK PQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3448 Collapse assay details

Data source: Panorama

Official Gene Symbol
Apobr
Peptide Sequence
LPGLHQALR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
8
Peptide End
16
CPTAC ID
non-CPTAC-3448
Peptide Molecular Mass
1,003.5927
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (3+) 5.9 3.7 2.8 8 6.6 3.7 9.9 7.6 4.6 15 15 15
y5 (2+) 7.1 3.8 3.3 11.4 5.9 3.7 13.4 7 5 15 15 15
y7 (2+) 5.8 3.1 5.3 8 3.3 5.7 9.9 4.5 7.8 15 15 15
y8 (2+) 5.6 2.9 4.1 7.7 3.9 4.6 9.5 4.9 6.2 15 15 15
y2 (1+) 40.9 28.9 32.6 53.8 32.8 28.8 67.6 43.7 43.5 15 15 15
sum 3.9 2.1 2.4 5.4 3.8 3.4 6.7 4.3 4.2 15 15 15


Additional Resources and Comments