Aga, CPTAC-3449 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Aga	N/A

Sequence Length (AA)	Molecular Weight (Da)
346	37022

Protein Name
N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MERKSNLSLL	LLLLVLGMPL	VRGSSPLPLV	VNTWPFKNAT	EAAWWTLLSG
60	70	80	90	100
GSALDAVENG	CAVCEKEQCD	GTVGFGGSPD	EGGETTLDAM	IMDGTAMDVG
110	120	130	140	150
AVGGLRRIKN	AIGVARRVLE	HTTHTLLVGD	SATKFAESMG	FTNEDLSTKT
160	170	180	190	200
SRDLHSDWLS	RNCQPNYWRN	VIPDPSKYCG	PYKPSGFLKQ	SISPHKEEVD
210	220	230	240	250
IHSHDTIGMV	VIHKTGHTAA	GTSTNGIKFK	IPGRVGDSPI	PGAGAYADDT
260	270	280	290	300
AGAAAATGDG	DTLLRFLPSY	QAVEYMRGGD	DPAIACQKVI	LRIQKYYPNF
310	320	330	340	346
FGAVICASVN	GSYGAACNKL	PTFTQFSFMV	SNSLHNEPTE	KKVDCI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3449 Collapse assay details

Data source: Panorama

Official Gene Symbol: Aga
Peptide Sequence: NAIGVAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 110
Peptide End: 116

CPTAC ID: non-CPTAC-3449
Peptide Molecular Mass: 699.4028
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b5 (2+)	52.8	25.4	29.1	59.7	42.7	50.6	79.7	49.7	58.4	15	15	15
y4 (2+)	134.3	113.7	101	112.9	167.8	145.5	175.5	202.7	177.1	15	15	15
y2 (1+)	23.3	9.3	18	35.3	17.9	22	42.3	20.2	28.4	15	15	15
y4 (1+)	7.4	10.3	5.3	9.6	9.5	6.3	12.1	14	8.2	15	15	15
y6 (1+)	30.6	16.8	14	31.5	16.5	21.6	43.9	23.5	25.7	15	15	15
sum	6.4	8.5	4.6	9.5	8	7.2	11.5	11.7	8.5	15	15	15

Aga

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3449 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Aga

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3449 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments