Bhmt, CPTAC-3450 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Bhmt	N/A

Sequence Length (AA)	Molecular Weight (Da)
407	45021

Protein Name
Betaine--homocysteine S-methyltransferase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAPVAGKKAK	KGILERLNAG	EVVIGDGGFV	FALEKRGYVK	AGPWTPEAAV
60	70	80	90	100
EHPEAVRQLH	REFLRAGSNV	MQTFTFYASE	DKLENRGNYV	AEKISGQKVN
110	120	130	140	150
EAACDIARQV	ADEGDALVAG	GVSQTPSYLS	CKSEVEVKKI	FRQQLEVFMK
160	170	180	190	200
KNVDFLIAEY	FEHVEEAVWA	VEALKASGKP	VAATMCIGPE	GDLHGVPPGE
210	220	230	240	250
CAVRLVKAGA	SIVGVNCHFD	PSVSLQTVKL	MKEGLEAARL	KAYLMSQPLA
260	270	280	290	300
YHTPDCGKQG	FIDLPEFPFG	LEPRVATRWD	IQKYAREAYN	LGVRYIGGCC
310	320	330	340	350
GFEPYHIRAI	AEELAPERGF	LPPASEKHGS	WGSGLDMHTK	PWIRARARKE
360	370	380	390	400
YWQNLRIASG	RPYNPSMSRP	DAWGVTKGAA	ELMQQKEATT	EQQLRELFEK
407
QKFKSAQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3450 Collapse assay details

Data source: Panorama

Official Gene Symbol: Bhmt
Peptide Sequence: EAYNLGVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 287
Peptide End: 294

CPTAC ID: non-CPTAC-3450
Peptide Molecular Mass: 920.4716
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (2+)	19.9	5.5	4.2	20.5	7.1	6.9	28.6	9	8.1	15	15	15
y3 (1+)	4.8	4.8	3.6	8.3	7.1	4.9	9.6	8.6	6.1	15	15	15
y4 (1+)	21.9	4.2	7.1	24.9	8.2	7	33.2	9.2	10	15	15	15
y5 (1+)	7.8	4.6	5.1	10.4	5.9	5.6	13	7.5	7.6	15	15	15
y6 (1+)	8	7.1	7.6	9.9	9.6	10.7	12.7	11.9	13.1	15	15	15
sum	6.2	2.2	2	9.9	5.3	3.6	11.7	5.7	4.1	15	15	15

Bhmt

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3450 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Bhmt

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3450 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments