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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Bhmt N/A
Sequence Length (AA) Molecular Weight (Da)
407 45021
Protein Name
Betaine--homocysteine S-methyltransferase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPVAGKKAK KGILERLNAG EVVIGDGGFV FALEKRGYVK AGPWTPEAAV
60 70 80 90 100
EHPEAVRQLH REFLRAGSNV MQTFTFYASE DKLENRGNYV AEKISGQKVN
110 120 130 140 150
EAACDIARQV ADEGDALVAG GVSQTPSYLS CKSEVEVKKI FRQQLEVFMK
160 170 180 190 200
KNVDFLIAEY FEHVEEAVWA VEALKASGKP VAATMCIGPE GDLHGVPPGE
210 220 230 240 250
CAVRLVKAGA SIVGVNCHFD PSVSLQTVKL MKEGLEAARL KAYLMSQPLA
260 270 280 290 300
YHTPDCGKQG FIDLPEFPFG LEPRVATRWD IQKYAREAYN LGVRYIGGCC
310 320 330 340 350
GFEPYHIRAI AEELAPERGF LPPASEKHGS WGSGLDMHTK PWIRARARKE
360 370 380 390 400
YWQNLRIASG RPYNPSMSRP DAWGVTKGAA ELMQQKEATT EQQLRELFEK
407
QKFKSAQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3450 Collapse assay details

Data source: Panorama

Official Gene Symbol
Bhmt
Peptide Sequence
EAYNLGVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
287
Peptide End
294
CPTAC ID
non-CPTAC-3450
Peptide Molecular Mass
920.4716
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 19.9 5.5 4.2 20.5 7.1 6.9 28.6 9 8.1 15 15 15
y3 (1+) 4.8 4.8 3.6 8.3 7.1 4.9 9.6 8.6 6.1 15 15 15
y4 (1+) 21.9 4.2 7.1 24.9 8.2 7 33.2 9.2 10 15 15 15
y5 (1+) 7.8 4.6 5.1 10.4 5.9 5.6 13 7.5 7.6 15 15 15
y6 (1+) 8 7.1 7.6 9.9 9.6 10.7 12.7 11.9 13.1 15 15 15
sum 6.2 2.2 2 9.9 5.3 3.6 11.7 5.7 4.1 15 15 15

Additional Resources and Comments