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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

C1rb

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
C1rb C1r
Sequence Length (AA) Molecular Weight (Da)
706 79936
Protein Name
Complement C1r-B subcomponent
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWLFALLVTL FYGVEGSIYL PQKLYGEVTS PLYPKPYPSD LETTTVITVP
60 70 80 90 100
MGYRVKLVFW QFDVEPSEGC LYDYVKISAD KQTLGRFCGQ LDSPLGNPPG
110 120 130 140 150
SKEFMSQGNK MLLTFHTDFS NEENGTIMFY KGFLAYYQAV DLDECASQPN
160 170 180 190 200
SVEEGLQPRC QHLCHNYVGG YFCSCHPGYE LQKDGQSCQA ECSSELYTEP
210 220 230 240 250
SGYVSSLEYP QPYPPDLRCN YSIRVERGLT VHLKFLDPFE IDDHQQVHCP
260 270 280 290 300
YDQLQIYANG KNLGEFCGKQ RPPDLDTSSN AVDLLFFTDE SGDSRGWKLH
310 320 330 340 350
YTTETIKCPQ PKALDEFTII QDPQPQYQFR DYFTVTCKQG YQLMEGNQAL
360 370 380 390 400
LSFTAVCQHD GTWHRAMPRC KIKNCGQPQS LSNGDFRYIT TKGVTTYEAS
410 420 430 440 450
IQYHCHEPYY KMLTRAGSSE SMRGIYTCTA QGIWKNEEEG EKMPRCLPVC
460 470 480 490 500
GKPVNPVTQK ERIIGGQPAR PGNFPWQAFT TIYGPGGGAL LGDRWILTAA
510 520 530 540 550
HTIYPKYPNK GKNTNPRTLV FLGHTNMEQI QKLGHHPVRR VIIHPDYRQE
560 570 580 590 600
EPDNFEGDIA LLELENSVTL GPELLPICLP DNETFYGQGL MGYVSGFGTT
610 620 630 640 650
GNRIPFHLRF VRLPVADREA CQRWLWTKKD TSPFSQNMFC SGDPAVQQDA
660 670 680 690 700
CQGDSGGVFA VRDRNRDIWV ATGIVSWGIG CGEGYGFYTK VLNYVDWIKK
706
EMGDEN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3451 Collapse assay details

Data source: Panorama

Official Gene Symbol
C1rb
Peptide Sequence
IIGGQPAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
463
Peptide End
470
CPTAC ID
non-CPTAC-3451
Peptide Molecular Mass
810.4712
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 21 4.8 2.9 28.3 8.1 5.1 35.2 9.4 5.9 15 15 15
y7 (2+) 43.3 8 9.8 47.6 10.9 9.2 64.3 13.5 13.4 15 15 15
y3 (1+) 54.6 7.1 5 62.4 8.4 9.2 82.9 11 10.5 15 15 15
y6 (1+) 5.7 3 1.7 7.2 4.8 4.6 9.2 5.7 4.9 15 15 15
y7 (1+) 17.5 5.8 3.7 19.1 6.3 4.9 25.9 8.6 6.1 15 15 15
sum 7.5 2.1 1.4 8.9 4.1 4 11.6 4.6 4.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments