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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Dsel N/A
Sequence Length (AA) Molecular Weight (Da)
1207 138868
Protein Name
Dermatan-sulfate epimerase-like protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAFMFTEHLL FLTLMMCSFS TCEESVSNYS EWAVFTDDIQ WLKSQKIQDF
60 70 80 90 100
KLNRRLHPNL YFDAGDIQTL KQKSRTSHLH IFRAIKSAVT IMLSNPSYYL
110 120 130 140 150
PPPKHAEFAA KWNEIYGNNL PPLALYCLLC PEDKVAFEFV MEYMDRMVSY
160 170 180 190 200
KDWLVENAPG DEVPVGHSLT GFATAFDFLY NLLGNQRKQK YLEKIWIVTE
210 220 230 240 250
EMYEYSKIRS WGKQLLHNHQ ATNMIALLIG ALVTGVDKGS KANIWKQVVV
260 270 280 290 300
DVMEKTMFLL KHIVDGSLDE GVAYGSYTSK SVTQYVFLAQ RHFNINNFDN
310 320 330 340 350
NWLKMHFWFY YATLLPGYQR TVGIADSNYN WFYGPESQLV FLDKFILQNG
360 370 380 390 400
AGNWLAQQIR KHRPKDGPMV PSTAQRWSTL HTEYIWYDPT LTPQPPVDFG
410 420 430 440 450
TAKMHTFPNW GVVTYGGGLP NTQTNTFVSF KSGKLGGRAV YDIVHFQPYS
460 470 480 490 500
WIDGWRSFNP GHEHPDQNSF TFAPNGQVFV SEALYGPKLS HLNNVLVFAP
510 520 530 540 550
SPSSQCNQPW EGQLGECAQW LKWTGEEVGD AAGEVITAAQ HGDRMFVSGE
560 570 580 590 600
AVSAYSSAMR LKSVYRALLL LNSQTLLVVD HIERQETSPI NSVSAFFHNL
610 620 630 640 650
DIDFKYIPYK FMNRYNGAMM DVWDAHYKMF WFDHHGNSPV ANIQEAEQAA
660 670 680 690 700
EFKKRWTQFV NVTFHMESTI TRIAYVFYGP YVNVSSCRFI DSSSSGLQIS
710 720 730 740 750
LHVNSTEHSV SVVTDYQNLK SRFSYLGFGG FASVANQGQI TRFGLGTQEI
760 770 780 790 800
VNPVRHDKVN FPFGFKFNIA VGFILCISLV ILTFQWRFYL SFRKLMRCVL
810 820 830 840 850
ILVIALWFIE LLDVWSTCTQ PICAKWTRTE AKANEKVMIS EGHHVDLPNV
860 870 880 890 900
IITSLPGSGA EILKQLFFNS SDFLYIRIPT AYMDIPETEF EIDSFVDACE
910 920 930 940 950
WKVSDIRSGH FHLLRGWLQS LVQDTKLHLQ NIHLHETSRS KLAQYFTTNK
960 970 980 990 1000
DKKRKLKRRE SLQDQRSRIK GPFDRDAEYI RALRRHLVYY PSARPVLSLS
1010 1020 1030 1040 1050
SGSWTLKLHF FQEVLGTSMR ALYIVRDPRA WIYSVLYGSK PSLYSLKNVP
1060 1070 1080 1090 1100
EHLAKLFKIE EGKSKCNSNS GYAFEYESLK KELEISQSNA ISLLSHLWVA
1110 1120 1130 1140 1150
NTAAALRINT DLLPTNYHLV KFEDIVHFPQ KTTERIFAFL GIPLSPASLN
1160 1170 1180 1190 1200
QMLFATSTNL FYLPYEGEIS PSNTNIWKTN LPRDEIKLIE NICWTLMDHL
1207
GYPKFMD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3457 Collapse assay details

Data source: Panorama

Official Gene Symbol
Dsel
Peptide Sequence
TSHLHIFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
76
Peptide End
83
CPTAC ID
non-CPTAC-3457
Peptide Molecular Mass
1,009.5457
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (2+) 8.6 23.1 22.7 17.5 28.9 27.4 19.5 37 35.6 14 15 15
y6 (2+) 7.9 4.1 6.6 12.7 5.3 7 15 6.7 9.6 14 15 15
y7 (2+) 4.3 3.5 12.9 6.3 4.9 9.9 7.6 6 16.3 14 15 15
y2 (1+) 9.6 8 8.1 11.8 7.2 10.6 15.2 10.8 13.3 14 15 15
y4 (1+) 4.1 4.7 4.2 7.2 7.3 4.2 8.3 8.7 5.9 14 15 15
sum 3.5 2.2 5.6 6 3.8 5.3 6.9 4.4 7.7 14 15 15


Additional Resources and Comments