Echs1, CPTAC-3460 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Echs1	N/A

Sequence Length (AA)	Molecular Weight (Da)
290	31474

Protein Name
Enoyl-CoA hydratase, mitochondrial

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAALRALLPR	ACSSLLSSVR	CPELRRFASG	ANFQYIITEK	KGKNSSVGLI
60	70	80	90	100
QLNRPKALNA	LCNGLIEELN	QALETFEQDP	AVGAIVLTGG	DKAFAAGADI
110	120	130	140	150
KEMQNRTFQD	CYSSKFLSHW	DHITRVKKPV	IAAVNGYALG	GGCELAMMCD
160	170	180	190	200
IIYAGEKAQF	GQPEILLGTI	PGAGGTQRLT	RAVGKSLAME	MVLTGDRISA
210	220	230	240	250
QDAKQAGLVS	KIFPVEKLVE	EAIQCAEKIA	SNSKIVVAMA	KESVNAAFEM
260	270	280	290
TLTEGNKLEK	RLFYSTFATD	DRREGMTAFV	EKRKANFKDH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3460 Collapse assay details

Data source: Panorama

Official Gene Symbol: Echs1
Peptide Sequence: LFYSTFATDDR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 262
Peptide End: 272

CPTAC ID: non-CPTAC-3460
Peptide Molecular Mass: 1,334.6143
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	8.2	14.2	5.2	15.1	18.3	19.7	17.2	23.2	20.4	15	15	15
y2 (1+)	18.3	12.1	4.9	9.7	24.5	15.7	20.7	27.3	16.4	15	15	15
y6 (1+)	11.3	8.3	6.4	16.3	13.8	18.9	19.8	16.1	20	15	15	15
y8 (1+)	12.9	10.6	7.6	18.5	17.3	17.8	22.6	20.3	19.4	15	15	15
y9 (1+)	11	10.2	5.7	12.4	19.3	15	16.6	21.8	16	15	15	15
sum	6.9	10.6	4.4	10.4	17.9	16.7	12.5	20.8	17.3	15	15	15

Echs1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3460 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Echs1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3460 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments