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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Ep400

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ep400 Kiaa1498
Sequence Length (AA) Molecular Weight (Da)
3072 337180
Protein Name
E1A-binding protein p400
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MHHGSGPQNV QHQLQRSRSF TGSEEEQPAH PNLPPSPAAP FAPSASPSAP
60 70 80 90 100
QSPGYQIQQL MSRSPVAGQN VNITLQNVGP VVGGNQQITL APLPLPNPTS
110 120 130 140 150
PGFQFGAQQR RFEHGSPSYI QVTSPMSQQV QTQSPTQPSP GPGQTLQNVR
160 170 180 190 200
AGAPGPGLGI CSNSPTGGFV DASVLVRQIS LSPSSGGHFV FQEAPGLTQM
210 220 230 240 250
AQGAQVQLQH SGAPITVRER RLSQPHAQSG GTIHHLGPQS PAAAGGTGLQ
260 270 280 290 300
PLASPNHITT ASLPPQISSI IQGQLIQQQQ QVLQGQPMNR SLGFERTPGV
310 320 330 340 350
LLPGVGGPSA FGMTSPPPPT SPSRTTMPPG LSSVPLTSMG SSGMKKVPKK
360 370 380 390 400
LEEIPPASQE MAQMRKQCLD YHYKEMEALK EVFKEYLIEL FFLQHLQGNM
410 420 430 440 450
MDFLAFKKKH YAPLQAYLRQ NDLDIEEEEE EEEEEEGKSE VINDEVKVVT
460 470 480 490 500
GKDGQTGTPV AIATQLPPNV SAAFSSQQQP FQHQSLTGSL VVGPGSATEA
510 520 530 540 550
DPFKRQQVMP PTEQSKRPRL EVGHPGVVFQ HPGVNAGVPL QQLMPTVQGG
560 570 580 590 600
MPPTPQATQL TGQKQSQQQY DPSTGPPVQN AASLHTPPPQ LPARLPPASV
610 620 630 640 650
PATALPSTLQ FSQQSQMVEA STQLQIPVKT QQLNAPIPAP LPSQLPAPSS
660 670 680 690 700
QPAQPALHVP MPGKAQMQTS QLSSQTQTVA STRPPLDSAQ PCQRSLPTSS
710 720 730 740 750
SSSSLVPVSG SGPGPSPARS SPVNRPSSAT NKALSPITSR SPGVAVSAPP
760 770 780 790 800
KPQSPAQNAA SSQDGSQDKL AEQITLENQI HQRIADLRKE GLWSLRRLPK
810 820 830 840 850
LQEAPRPKSH WDYLLEEMQW MATDFAQERR WKLAAAKKLV RTVARHHEEK
860 870 880 890 900
KLREERGKKE EQSRLRRIAA TTAREIEYFW SNIEQVVEIK LQVELEEKRK
910 920 930 940 950
KALNLQKVSR RGKESRLKGF DTSPEHSLDL GISGRKRKAS TSLTDDEVED
960 970 980 990 1000
EEETIEEEEA HEGLVDHHTE LTNLAKEAEL PLIDLMKLYE GAFLPNFQWP
1010 1020 1030 1040 1050
QPEPDHEESS GEEDVEDCPS DRESRRDSVL IDSLFIMDQF KAAERMSIGK
1060 1070 1080 1090 1100
SNTKDITEVT AVAEAILPKG SARVTTAVKF SAPSLLYGAL RDYQKIGLDW
1110 1120 1130 1140 1150
LAKLYRKNLN GILADEAGLG KTVQIIAFFA HLACNEGNWG PHLVVMRSCN
1160 1170 1180 1190 1200
ILKWELELKR WCPGLKTLSY VGSHRELKAK RQEWTEPNNF HICITSYKQF
1210 1220 1230 1240 1250
FRGYTAFSRV HWKCLVVDEM QRVKGMTERH WEAIFKLQSQ QRLLLIDVPL
1260 1270 1280 1290 1300
HNTFLELWTM VHFLIPGISR PYLSFPLKAP NEENQDYYHK MVIRLHRVTQ
1310 1320 1330 1340 1350
PFILRRTKRD VEKQLTRKYE HVLKCRLSSR QKALYEDVIL QPRTQEALKS
1360 1370 1380 1390 1400
GHFVSVLSVL TRLQRICNHP GLVEPRVPGS SFAAGSLQYK SASLILRVLE
1410 1420 1430 1440 1450
REFWKETDLS IFDLIGLENK ITRHEAELLC KKKVTRKLME EVFASPPPSA
1460 1470 1480 1490 1500
RPAAVKLKAS RLFQPVQYGQ KPEGRTVAFP STHPPRMANT NTSTATPQGQ
1510 1520 1530 1540 1550
VRGRPPIATF SANPDTKGGE VVKIAQLASI AGPQSRVAQP ETPVTLQFQG
1560 1570 1580 1590 1600
NKFTLSHSQL RQLTAGQPLQ LQGSVLQIVS APGQPYLRAP GPVVMQTVSQ
1610 1620 1630 1640 1650
AGAVHSTLGS KPPTSGPSPA PLTPQVGVPG RVAVSAMAVG EPGLASKPAS
1660 1670 1680 1690 1700
PAAGPTQEEK SRLLKERLDQ IHFINERRCS QAPVYGRDLL RICSLPGRRK
1710 1720 1730 1740 1750
RPLCWSLDSN FGKGPKGVNY DMSLSKSEGD LILTLSQESL QDVLGRVACV
1760 1770 1780 1790 1800
IPPVVATPPS LWVARPPSLY SSRLRALRQC LREHTGPYHR QLQQLTALRS
1810 1820 1830 1840 1850
LQFPELRLVQ FDSGKLEALA ILLQKLKSEG RRVLILSQMV LMLDILEMFL
1860 1870 1880 1890 1900
NFHYLTYVRI DENANSEQRQ ELMRSFNRDR RIFCALLSTH SRATGINLVE
1910 1920 1930 1940 1950
ADTVVFYDND LNPVMDAKAQ EWCDRIGRCK DIHIYRLVSG NSIEEKLLKN
1960 1970 1980 1990 2000
GTKDLIREVA AQGNDYSMAF LTQRTIQELF EVYSPMDDTG FPVKAEEFVV
2010 2020 2030 2040 2050
LSQEPSVSET IAPKIARPFI EALKSIECLE EDAQRSTEEA VPGSSSVAVS
2060 2070 2080 2090 2100
SDSDGSRYDE EPSQLEELAD FMEQLTPIEK YALNYLELFH TTTEQEKERI
2110 2120 2130 2140 2150
SEDLVMASMK DWETRNARAL QEREARLQLE QEEAELLTYT REDAYTMEYV
2160 2170 2180 2190 2200
YEDADGQTEV MPLWTPPTPP QDDNDIYIDS VMCLMYETTP IPEAKLPPVY
2210 2220 2230 2240 2250
VRKERKRHKT DPSAAGRKKK QRHGEAVVPP RSLFDRATPG MLKIRREGKE
2260 2270 2280 2290 2300
QKKNLLLKQQ TPFAKPLPTY VKSSGEPAQD SPDWLIGEDW ALLQAVKQLL
2310 2320 2330 2340 2350
ELPLNLTIVS PAHTPNWDLV SDVVNSCSRI YRSSKQCRNR YENVIIPREE
2360 2370 2380 2390 2400
GKSKNNRPLR TSQIYAQDEN ATHTQLYTSH FELMKMTAGK RSPPIKPLLG
2410 2420 2430 2440 2450
MNPFQKNPKH ASVLAESGIN YDKPLPPIQV ASLRAERIAK EKKALADQQK
2460 2470 2480 2490 2500
AQQPPVTQPP PQQQQQQQQQ QQQQQQQQQP PPPPQQPPPP VPQPQAASSQ
2510 2520 2530 2540 2550
TPAGQPAVQP QPQPQVQTQP QPVQPQSKGQ PTMTTVGSAA VLAGTIKTSV
2560 2570 2580 2590 2600
TGTSIPTGTV SGNVIVNTIA GVPAATFQSI NKRLASPVAP GTLTTSGGSA
2610 2620 2630 2640 2650
PAQVVHTQQR AVGSPATATT DLVSMTTTQG VRAVTSVTAS AVVTTNLTPV
2660 2670 2680 2690 2700
QTPTRSLVTQ VSQATGVQLP GKTITPAAHF QLLRQQQQQQ QQQQQQQQTS
2710 2720 2730 2740 2750
QVQVPQLQSQ AQSPAQIKAV SKLGPEHIIK MQKQKMQLPP QPPPPQAQPG
2760 2770 2780 2790 2800
PPQQPAQVQV QTPQPPQQQQ SPQLTTVTAP RPGALLTGTT VTNLQVARLT
2810 2820 2830 2840 2850
RVPTSQLQAQ GQMQTQTPQP AQVALAKPPV VSVPAAVVSS PGVTTLPMNV
2860 2870 2880 2890 2900
AGISVAIGQP QKTAGQTVVA QPVNVQQLLK YKQQTAVQQQ KAIQPQVAQG
2910 2920 2930 2940 2950
QAAVQQKLTT QQITTQGPQQ KVAYAAQPAL KTQFLTTPIS QAQKLAGTQQ
2960 2970 2980 2990 3000
VQTQIQVAKL PQVVQQQTPV ASIQQVASAS QQASPQTVTL TQATAAGQQV
3010 3020 3030 3040 3050
QMIPTVTATA QLVQQKLIQQ QVVTTASASL QTPGGPSPAQ LPASSDSPSQ
3060 3070 3072
QPKLQMRVPA VRLKTPTKPP CQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3461 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ep400
Peptide Sequence
ALYEDVILQPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1333
Peptide End
1343
CPTAC ID
non-CPTAC-3461
Peptide Molecular Mass
1,315.7136
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 3 4.1 4.7 7.2 7.5 5.8 7.8 8.5 7.5 15 15 15
y3 (1+) 6 3.6 5.5 7.7 4.2 5.4 9.8 5.5 7.7 15 15 15
y4 (1+) 6.7 3.8 2.9 6 6.7 4.6 9 7.7 5.4 15 15 15
y5 (1+) 5.9 4 3.9 6.1 5.6 4.6 8.5 6.9 6 15 15 15
y7 (1+) 7.3 3.2 2.9 8.4 8.2 6.4 11.1 8.8 7 15 15 15
sum 2.9 2 2.3 5.6 5.3 3.2 6.3 5.7 3.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments