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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Krt6b K6-beta, Krt2-6b
Sequence Length (AA) Molecular Weight (Da)
562 60322
Protein Name
Keratin, type II cytoskeletal 6B
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSTKTTIKSQ TSHRGYSASS ARVPGLNRSG FSSVSVCRSR GSGGSSAMCG
60 70 80 90 100
GAGFGSRSLY GVGSSKRISI GGGSCGIGGG YGSRFGGAYG SRFGGSFGIG
110 120 130 140 150
GGAGSGFGFG GGVGFGGGYG GAGFPVCPPG GIQEVTINQN LLTPLNVQID
160 170 180 190 200
PTIQRVRTEE REQIKTLNNK FASFIDKVRF LEQQNKVLDT KWALLQEQGT
210 220 230 240 250
KTVRQNLEPM FEQYISNLRR QLDSIIGERG RLDSELRNMQ DTVEDYKSKY
260 270 280 290 300
EDEINKRTKA ENEFVTVKKD VDAAYMTKVE LQAKADSLAD EINFLRVIYE
310 320 330 340 350
AELSQMQTHI SDTSVVLSMD NNRSLDLDSI IAEVKAQYED IAQRSRAEAE
360 370 380 390 400
SWYQTKYEEL QVTAGRHGDD LRNTKQEIAE INRMIQRLRS EIDHVKKQCA
410 420 430 440 450
NLQAAIADAE QRGEMALKDA RGKLEGLEDA LQKAKQEMAR LLKEYQELMN
460 470 480 490 500
VKLALDVEIA TYRKLLEGEE CRLNGEGVGP VNISVVQSTV SSGYGSAGGA
510 520 530 540 550
SSSLGLGGSS SYSYGSSHGL GGGFSAGSGR AIGGGLSSSG GLSSSTIKYT
560 562
TSASSSRKSY RH

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3462 Collapse assay details

Data source: Panorama

Official Gene Symbol
Krt6b
Peptide Sequence
ADSLADEINFLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
285
Peptide End
296
CPTAC ID
non-CPTAC-3462
Peptide Molecular Mass
1,362.6779
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 7 2.9 2.3 24 20.8 10.2 25 21 10.5 15 15 15
y7 (1+) 5.8 3 4.5 8.4 5.9 6.8 10.2 6.6 8.2 15 15 15
y6 (1+) 7.8 5.5 4.2 8.9 5.7 7 11.8 7.9 8.2 15 15 15
y4 (1+) 5.4 3.1 3.9 7.2 4.9 4.8 9 5.8 6.2 15 15 15
b3 (1+) 3.4 3 2.1 6.5 4.8 3.3 7.3 5.7 3.9 15 15 15
sum 4.5 1.9 1.6 12.8 11.4 4.9 13.6 11.6 5.2 15 15 15


Additional Resources and Comments