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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Krt15 Krt1-15
Sequence Length (AA) Molecular Weight (Da)
452 49138
Protein Name
Keratin, type I cytoskeletal 15
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MATTFLQTSS TFGGSSTRGA SLRAGGGSFG GGSLYGGGGS RSISASSARF
60 70 80 90 100
VSSGAGGGFG GGMSCGFGGG FGGGFGGGFG GGFGDFGGGD GGLLSGNEKV
110 120 130 140 150
TMQNLNDRLA SYLDKVRALE QANTELEVKI RDWYQKQSPA SPDRDYSHYF
160 170 180 190 200
KTMEEIRDKI LAATIDNSRV VLEIDNARLA ADDFRLKYEN ELTLRQGVEA
210 220 230 240 250
DINGLRRVLD ELTLARTDLE MQIEQLNEEL AYLKKNHEEE MKEFSSQLAG
260 270 280 290 300
QVNVEMDAAP GVDLTRMLAE MREQYEAIAE KNRRDVEAWF FSKTEELNKE
310 320 330 340 350
VASNTEMIQT SKTEITDLRR TLQGLEIELQ SQLSMKAGLE NSLAEVECRY
360 370 380 390 400
ATQLQQIQGV ITGLETQLSE LRCEMEAQNQ EYNMLLDIKT RLEQEIATYR
410 420 430 440 450
NLLEGQDAKM AGIGVREGSS GGGGSSSSSS NFHISVEESV DGKVVSSRKR
452
EI

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3463 Collapse assay details

Data source: Panorama

Official Gene Symbol
Krt15
Peptide Sequence
ILAATIDNSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
160
Peptide End
169
CPTAC ID
non-CPTAC-3463
Peptide Molecular Mass
1,072.5877
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 5.5 3.8 2.7 12.1 5 3.2 13.3 6.3 4.2 15 15 15
y7 (1+) 10.3 6.3 2.9 10.2 7.8 2.6 14.5 10 3.9 15 15 15
y6 (1+) 10.8 5.2 5.3 15.7 7.4 6.4 19.1 9 8.3 15 15 15
y3 (1+) 6.2 6.3 3.2 6.8 7 5 9.2 9.4 5.9 15 15 15
b2 (1+) 11.9 4.1 5.3 9.6 6.2 5.5 15.3 7.4 7.6 15 15 15
sum 4.3 2.5 2 8.5 4.6 2.8 9.5 5.2 3.4 15 15 15


Additional Resources and Comments