Krt15, CPTAC-3463 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Krt15	Krt1-15

Sequence Length (AA)	Molecular Weight (Da)
452	49138

Protein Name
Keratin, type I cytoskeletal 15

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MATTFLQTSS	TFGGSSTRGA	SLRAGGGSFG	GGSLYGGGGS	RSISASSARF
60	70	80	90	100
VSSGAGGGFG	GGMSCGFGGG	FGGGFGGGFG	GGFGDFGGGD	GGLLSGNEKV
110	120	130	140	150
TMQNLNDRLA	SYLDKVRALE	QANTELEVKI	RDWYQKQSPA	SPDRDYSHYF
160	170	180	190	200
KTMEEIRDKI	LAATIDNSRV	VLEIDNARLA	ADDFRLKYEN	ELTLRQGVEA
210	220	230	240	250
DINGLRRVLD	ELTLARTDLE	MQIEQLNEEL	AYLKKNHEEE	MKEFSSQLAG
260	270	280	290	300
QVNVEMDAAP	GVDLTRMLAE	MREQYEAIAE	KNRRDVEAWF	FSKTEELNKE
310	320	330	340	350
VASNTEMIQT	SKTEITDLRR	TLQGLEIELQ	SQLSMKAGLE	NSLAEVECRY
360	370	380	390	400
ATQLQQIQGV	ITGLETQLSE	LRCEMEAQNQ	EYNMLLDIKT	RLEQEIATYR
410	420	430	440	450
NLLEGQDAKM	AGIGVREGSS	GGGGSSSSSS	NFHISVEESV	DGKVVSSRKR
452
EI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3463 Collapse assay details

Data source: Panorama

Official Gene Symbol: Krt15
Peptide Sequence: ILAATIDNSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 160
Peptide End: 169

CPTAC ID: non-CPTAC-3463
Peptide Molecular Mass: 1,072.5877
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	5.5	3.8	2.7	12.1	5	3.2	13.3	6.3	4.2	15	15	15
y7 (1+)	10.3	6.3	2.9	10.2	7.8	2.6	14.5	10	3.9	15	15	15
y6 (1+)	10.8	5.2	5.3	15.7	7.4	6.4	19.1	9	8.3	15	15	15
y3 (1+)	6.2	6.3	3.2	6.8	7	5	9.2	9.4	5.9	15	15	15
b2 (1+)	11.9	4.1	5.3	9.6	6.2	5.5	15.3	7.4	7.6	15	15	15
sum	4.3	2.5	2	8.5	4.6	2.8	9.5	5.2	3.4	15	15	15

Krt15

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3463 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Krt15

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3463 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments