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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Leg1 N/A
Sequence Length (AA) Molecular Weight (Da)
337 38284
Protein Name
Protein LEG1 homolog
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAVLASWVWV LAGCFCAAVA EVSDSSDPYP PLWEDSPEQL SDYMMEDGNY
60 70 80 90 100
IINPWVYTDR MGMYRILLEE TAMYFAKYGP ENEQNLLWGL PLQFGWQYQS
110 120 130 140 150
GRLADPTGMT DCGNELNESL CVSVDSWWAD INYYLSVIPF LAAVDSGITG
160 170 180 190 200
ISPNQITILP PPKDQMRFCY NVSDCQSAVP GTMNRWRDFF QYMQLNSSDF
210 220 230 240 250
DGLLNYLWEA HASSLDYPTS AFGDRYNFYS EKEANFEENW AIAVNYLAAA
260 270 280 290 300
RLPTTQNRTY SFQRGLPPRV LVDTDIAPFI PDFTPLQNEV LVSLKLLGDT
310 320 330 337
DRNSGSLSLT LWENLMSTKL ARTLFLKAFE EFLATSS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3469 Collapse assay details

Data source: Panorama

Official Gene Symbol
Leg1
Peptide Sequence
LLGDTDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
296
Peptide End
302
CPTAC ID
non-CPTAC-3469
Peptide Molecular Mass
788.4028
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 75.6 43.4 15.5 136.4 42.3 35 155.9 60.6 38.3 15 15 15
y6 (2+) 47.5 15.6 21.6 56.1 17.6 20.3 73.5 23.5 29.6 15 15 15
y4 (1+) 75.6 73.8 74.8 118.6 125.3 78.7 140.6 145.4 108.6 15 15 15
y5 (1+) 122 64.7 75 188.6 90.9 122.1 224.6 111.6 143.3 15 15 15
y6 (1+) 5.9 6.8 8.2 12.6 14.1 13.9 13.9 15.7 16.1 15 15 15
sum 5.8 6.2 7.2 11.3 11.1 11.7 12.7 12.7 13.7 15 15 15


Additional Resources and Comments