Pm20d1, CPTAC-3475 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Pm20d1	N/A

Sequence Length (AA)	Molecular Weight (Da)
503	55663

Protein Name
N-fatty-acyl-amino acid synthase/hydrolase PM20D1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAELLASLPA	WAAVLLLFFA	TVSGSTGPRS	RENRGASRIP	SQFSEEERVA
60	70	80	90	100
IKEALKGAIQ	IPTVSFSHEE	SNTTALAEFG	EYIRKAFPTV	FHSSLVQHEV
110	120	130	140	150
VAKYSHLFTI	QGSDPSLQPY	MLMAHIDVVP	APEEGWEVPP	FSGLERNGFI
160	170	180	190	200
YGRGALDNKN	SVMAILHALE	LLLIRNYSPK	RSFFIALGHD	EEVSGEKGAQ
210	220	230	240	250
KISALLQARG	VQLAFLVDEG	SFILEGFIPN	LEKPVAMISV	TEKGALDLML
260	270	280	290	300
QVNMTPGHSS	APPKETSIGI	LSAAVSRLEQ	TPMPNMFGGG	PLKKTMKLLA
310	320	330	340	350
NEFSFPINIV	LRNLWLFHPI	VSRIMERNPI	TNALVRTTTA	LTMFNAGIKV
360	370	380	390	400
NVIPPLAQAT	INCRIHPSQT	VHEVLELVKN	TVADDRVQLH	VLRSFEPLPI
410	420	430	440	450
SPSDDQAMGY	QLLQETIRSV	FPEVDIVVPG	ICIANTDTRH	YANITNGMYR
460	470	480	490	500
FNPLPLNPQD	FSGVHGINEK	VSVQNYQNQV	KFIFEFIQNA	DTYKEPVPHL
503
HEL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3475 Collapse assay details

Data source: Panorama

Official Gene Symbol: Pm20d1
Peptide Sequence: NPITNALVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 328
Peptide End: 336

CPTAC ID: non-CPTAC-3475
Peptide Molecular Mass: 996.5716
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	6	2.9	1.6	5.9	6.2	2.9	8.4	6.8	3.3	15	15	15
y8 (2+)	4.8	2.2	2.4	9.7	4.4	4.2	10.8	4.9	4.8	15	15	15
y4 (1+)	8.8	2.7	3.3	14.8	6.1	4.2	17.2	6.7	5.3	15	15	15
y5 (1+)	5.3	2.6	3.3	11	6.9	3.7	12.2	7.4	5	15	15	15
y6 (1+)	5.1	2.2	1.5	10.9	4.1	3.1	12	4.7	3.4	15	15	15
sum	2.6	0.9	0.7	8.7	4.4	3	9.1	4.5	3.1	15	15	15

Pm20d1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3475 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Pm20d1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3475 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments