Minpp1, CPTAC-3474 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Minpp1	Mipp

Sequence Length (AA)	Molecular Weight (Da)
481	54537

Protein Name
Multiple inositol polyphosphate phosphatase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLRGARSHLP	ASVAPAAVLA	AALLSSFARC	SLPGRGDPVA	SVLSPYFGTK
60	70	80	90	100
TRYEDANPWL	LVDPVAPRRD	PELLAGTCTP	VQLVALIRHG	TRYPTTKQIR
110	120	130	140	150
KLKQLQGLLQ	TRESRDGGSQ	VAAALAEWPL	WYGDWMDGQL	VEKGRQDMRQ
160	170	180	190	200
LALRLAALFP	DLFSRENYDR	LRLITSSKHR	CVDSSAAFLQ	GLWQHYHPGL
210	220	230	240	250
PPPDVSDMEC	GPPRINDKLM	RFFDHCEKFL	TDVERNETAL	YHVEAFKTGP
260	270	280	290	300
EMQKVLKKVA	ATLQVPMNSL	NADLIQVAFF	TCSFDLAIKG	VHSPWCDVFD
310	320	330	340	350
VDDARVLEYL	NDLKQYWKRS	YGYTINSRSS	CNLFQDIFLH	LDKAVEQKQR
360	370	380	390	400
SQPVSSPVIL	QFGHAETLLP	LLSLMGYFKD	KEPLTAYNFE	EQVNRKFRSG
410	420	430	440	450
HIVPYASNLI	FVLYHCDNAQ	SPEEQFQIQL	LLNEKVLPLA	HSQRPVGLYE
460	470	480	481
ELKTHYRDIL	QSCQTSKECS	PPKANITSDE	L

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1313 Collapse assay details

Data source: Panorama

Official Gene Symbol: MINPP1
Peptide Modified Sequence: N[+1.0]ATALYHVEAFK
Modification Type: Deamidated (N)
Protein - Site of Modification: 242
Peptide - Site of Modification: 1
Peptide Start: 242
Peptide End: 253

CPTAC ID: CPTAC-1313
Peptide Molecular Mass: 1,363.6772
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (2+)	64.4	22.9	22.3	58.1	23.2	29.3	86.7	32.6	36.8	15	15	15
y9 (2+)	43.7	22.9	19.2	44.1	27.5	32.2	62.1	35.8	37.5	15	15	15
y8 (2+)	64.7	31.1	40	61.5	27.1	25.5	89.3	41.3	47.4	15	15	15
sum	22	14	15	22	14.6	18.6	31.1	20.2	23.9	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3474 Collapse assay details

Data source: Panorama

Official Gene Symbol: Minpp1
Peptide Sequence: SYGYTINSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 320
Peptide End: 328

CPTAC ID: non-CPTAC-3474
Peptide Molecular Mass: 1,059.4985
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers