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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MINPP1 MIPP
Sequence Length (AA) Molecular Weight (Da)
487 55051
Protein Name
Multiple inositol polyphosphate phosphatase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLRAPGCLLR TSVAPAAALA AALLSSLARC SLLEPRDPVA SSLSPYFGTK
60 70 80 90 100
TRYEDVNPVL LSGPEAPWRD PELLEGTCTP VQLVALIRHG TRYPTVKQIR
110 120 130 140 150
KLRQLHGLLQ ARGSRDGGAS STGSRDLGAA LADWPLWYAD WMDGQLVEKG
160 170 180 190 200
RQDMRQLALR LASLFPALFS RENYGRLRLI TSSKHRCMDS SAAFLQGLWQ
210 220 230 240 250
HYHPGLPPPD VADMEFGPPT VNDKLMRFFD HCEKFLTEVE KNATALYHVE
260 270 280 290 300
AFKTGPEMQN ILKKVAATLQ VPVNDLNADL IQVAFFTCSF DLAIKGVKSP
310 320 330 340 350
WCDVFDIDDA KVLEYLNDLK QYWKRGYGYT INSRSSCTLF QDIFQHLDKA
360 370 380 390 400
VEQKQRSQPI SSPVILQFGH AETLLPLLSL MGYFKDKEPL TAYNYKKQMH
410 420 430 440 450
RKFRSGLIVP YASNLIFVLY HCENAKTPKE QFRVQMLLNE KVLPLAYSQE
460 470 480 487
TVSFYEDLKN HYKDILQSCQ TSEECELARA NSTSDEL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1313 Collapse assay details

Data source: Panorama

Official Gene Symbol
MINPP1
Peptide Modified Sequence
N[+1.0]ATALYHVEAFK
Modification Type
Deamidated (N)
Protein - Site of Modification
242
Peptide - Site of Modification
1
Peptide Start
242
Peptide End
253
CPTAC ID
CPTAC-1313
Peptide Molecular Mass
1,363.6772
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (2+) 64.4 22.9 22.3 58.1 23.2 29.3 86.7 32.6 36.8 15 15 15
y9 (2+) 43.7 22.9 19.2 44.1 27.5 32.2 62.1 35.8 37.5 15 15 15
y8 (2+) 64.7 31.1 40 61.5 27.1 25.5 89.3 41.3 47.4 15 15 15
sum 22 14 15 22 14.6 18.6 31.1 20.2 23.9 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3474 Collapse assay details

Data source: Panorama

Official Gene Symbol
Minpp1
Peptide Sequence
SYGYTINSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
320
Peptide End
328
CPTAC ID
non-CPTAC-3474
Peptide Molecular Mass
1,059.4985
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 10.6 10.5 12.6 14.5 14.1 18.7 18 17.6 22.5 11 12 12
y4 (1+) 28.5 16.3 19 30.2 24.2 18.3 41.5 29.2 26.4 11 12 12
y5 (1+) 7 8.2 8.2 10.9 9.8 12.8 13 12.8 15.2 11 12 12
y6 (1+) 16.7 18.6 19.5 13.3 17.4 19 21.3 25.5 27.2 11 12 12
y7 (1+) 6.3 7.3 5.2 7.6 8.3 6 9.9 11.1 7.9 11 12 12
sum 5.5 6 3.5 8.7 8.5 6.2 10.3 10.4 7.1 11 12 12


Additional Resources and Comments