MINPP1, CPTAC-1313 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MINPP1	MIPP

Sequence Length (AA)	Molecular Weight (Da)
487	55051

Protein Name
Multiple inositol polyphosphate phosphatase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLRAPGCLLR	TSVAPAAALA	AALLSSLARC	SLLEPRDPVA	SSLSPYFGTK
60	70	80	90	100
TRYEDVNPVL	LSGPEAPWRD	PELLEGTCTP	VQLVALIRHG	TRYPTVKQIR
110	120	130	140	150
KLRQLHGLLQ	ARGSRDGGAS	STGSRDLGAA	LADWPLWYAD	WMDGQLVEKG
160	170	180	190	200
RQDMRQLALR	LASLFPALFS	RENYGRLRLI	TSSKHRCMDS	SAAFLQGLWQ
210	220	230	240	250
HYHPGLPPPD	VADMEFGPPT	VNDKLMRFFD	HCEKFLTEVE	KNATALYHVE
260	270	280	290	300
AFKTGPEMQN	ILKKVAATLQ	VPVNDLNADL	IQVAFFTCSF	DLAIKGVKSP
310	320	330	340	350
WCDVFDIDDA	KVLEYLNDLK	QYWKRGYGYT	INSRSSCTLF	QDIFQHLDKA
360	370	380	390	400
VEQKQRSQPI	SSPVILQFGH	AETLLPLLSL	MGYFKDKEPL	TAYNYKKQMH
410	420	430	440	450
RKFRSGLIVP	YASNLIFVLY	HCENAKTPKE	QFRVQMLLNE	KVLPLAYSQE
460	470	480	487
TVSFYEDLKN	HYKDILQSCQ	TSEECELARA	NSTSDEL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1313 Collapse assay details

Data source: Panorama

Official Gene Symbol: MINPP1
Peptide Modified Sequence: N[+1.0]ATALYHVEAFK
Modification Type: Deamidated (N)
Protein - Site of Modification: 242
Peptide - Site of Modification: 1
Peptide Start: 242
Peptide End: 253

CPTAC ID: CPTAC-1313
Peptide Molecular Mass: 1,363.6772
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D

CPTAC-1297: SUN1. TLSPTGN[+1.0]ISSAPK	CPTAC-1298: COLGALT1. TALWVATDHNM[+16.0]DN[+1.0]TSTVLR	CPTAC-1299: APOB. FN[+1.0]SSYLQGTNQITGR
CPTAC-1300: APOB. FVEGSHN[+1.0]STVSLTTK	CPTAC-1301: QPCT. YFQN[+1.0]YSYGGVIQDDHIPFLR	CPTAC-1302: CA1. EIINVGHSFHVNFEDNDN[+1.0]R
CPTAC-1303: VIM. QDVDN[+1.0]ASLAR	CPTAC-1304: DPP7. N[+1.0]LSASVIAVTIQGGAHHLDLR	CPTAC-1305: SERPING1. M[+16.0]LFVEPILEVSSLPTTN[+1.0]STTNSATK
CPTAC-1306: CEACAM8. LFIPN[+1.0]ITTK	CPTAC-1307: DSG2. ESFLAPSSGVQPTLAM[+16.0]PNIAVGQN[+1.0]VTVTER	CPTAC-1308: M6PR. LKPLFN[+1.0]K
CPTAC-1309: ACP2. FNPN[+1.0]ISWQPIPVHTVPITEDR	CPTAC-1310: PCDH1. AN[+1.0]DSDQGANAEIEYTFHQAPEVVR	CPTAC-1311: ITGB8. NYAIIKPIGFN[+1.0]ETAK
CPTAC-1312: CALCOCO2. SAN[+1.0]STLAQALHWTR	CPTAC-1313: MINPP1. N[+1.0]ATALYHVEAFK	CPTAC-1314: KRT40. TYN[+1.0]ASVLPVR
CPTAC-1315: MMP23B. ALM[+16.0]HLN[+1.0]ATLR	CPTAC-1316: CD6. EGDN[+1.0]ITLK	CPTAC-1317: CD6. N[+1.0]ATVVWM[+16.0]K
CPTAC-1318: CD6. TVNSLN[+1.0]VSAISIPEHDEADEISDENR	CPTAC-1319: IRAK2. TILVDN[+1.0]NTWNNTHISR	CPTAC-1320: AEN. LN[+1.0]STAQK
CPTAC-1321: PLTP. N[+1.0]WSLPNR	CPTAC-1322: OVGP1. FVPIYGN[+1.0]HSSVNSVTPQTSPLSLK	CPTAC-2612: MMP23B. ALM[+16.0]HLN[+1.0]ATLR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (2+)	64.4	22.9	22.3	58.1	23.2	29.3	86.7	32.6	36.8	15	15	15
y9 (2+)	43.7	22.9	19.2	44.1	27.5	32.2	62.1	35.8	37.5	15	15	15
y8 (2+)	64.7	31.1	40	61.5	27.1	25.5	89.3	41.3	47.4	15	15	15
sum	22	14	15	22	14.6	18.6	31.1	20.2	23.9	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3474 Collapse assay details

Data source: Panorama

Official Gene Symbol: Minpp1
Peptide Sequence: SYGYTINSR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 320
Peptide End: 328

CPTAC ID: non-CPTAC-3474
Peptide Molecular Mass: 1,059.4985
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers