ITGB8, CPTAC-1311 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ITGB8	N/A

Sequence Length (AA)	Molecular Weight (Da)
769	85632

Protein Name
Integrin beta-8

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MCGSALAFFT	AAFVCLQNDR	RGPASFLWAA	WVFSLVLGLG	QGEDNRCASS
60	70	80	90	100
NAASCARCLA	LGPECGWCVQ	EDFISGGSRS	ERCDIVSNLI	SKGCSVDSIE
110	120	130	140	150
YPSVHVIIPT	ENEINTQVTP	GEVSIQLRPG	AEANFMLKVH	PLKKYPVDLY
160	170	180	190	200
YLVDVSASMH	NNIEKLNSVG	NDLSRKMAFF	SRDFRLGFGS	YVDKTVSPYI
210	220	230	240	250
SIHPERIHNQ	CSDYNLDCMP	PHGYIHVLSL	TENITEFEKA	VHRQKISGNI
260	270	280	290	300
DTPEGGFDAM	LQAAVCESHI	GWRKEAKRLL	LVMTDQTSHL	ALDSKLAGIV
310	320	330	340	350
VPNDGNCHLK	NNVYVKSTTM	EHPSLGQLSE	KLIDNNINVI	FAVQGKQFHW
360	370	380	390	400
YKDLLPLLPG	TIAGEIESKA	ANLNNLVVEA	YQKLISEVKV	QVENQVQGIY
410	420	430	440	450
FNITAICPDG	SRKPGMEGCR	NVTSNDEVLF	NVTVTMKKCD	VTGGKNYAII
460	470	480	490	500
KPIGFNETAK	IHIHRNCSCQ	CEDNRGPKGK	CVDETFLDSK	CFQCDENKCH
510	520	530	540	550
FDEDQFSSES	CKSHKDQPVC	SGRGVCVCGK	CSCHKIKLGK	VYGKYCEKDD
560	570	580	590	600
FSCPYHHGNL	CAGHGECEAG	RCQCFSGWEG	DRCQCPSAAA	QHCVNSKGQV
610	620	630	640	650
CSGRGTCVCG	RCECTDPRSI	GRFCEHCPTC	YTACKENWNC	MQCLHPHNLS
660	670	680	690	700
QAILDQCKTS	CALMEQQHYV	DQTSECFSSP	SYLRIFFIIF	IVTFLIGLLK
710	720	730	740	750
VLIIRQVILQ	WNSNKIKSSS	DYRVSASKKD	KLILQSVCTR	AVTYRREKPE
760	769
EIKMDISKLN	AHETFRCNF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1311 Collapse assay details

Data source: Panorama

Official Gene Symbol: ITGB8
Peptide Modified Sequence: NYAIIKPIGFN[+1.0]ETAK
Modification Type: Deamidated (N)
Protein - Site of Modification: 456
Peptide - Site of Modification: 11
Peptide Start: 446
Peptide End: 460

CPTAC ID: CPTAC-1311
Peptide Molecular Mass: 1,678.8930
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human ovarian tumor tissue digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Stable isotope-labeled peptides
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Accela 1250 Quaternary Low Pump
Column Packing: C18, 3 µm, 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-D

CPTAC-1297: SUN1. TLSPTGN[+1.0]ISSAPK	CPTAC-1298: COLGALT1. TALWVATDHNM[+16.0]DN[+1.0]TSTVLR	CPTAC-1299: APOB. FN[+1.0]SSYLQGTNQITGR
CPTAC-1300: APOB. FVEGSHN[+1.0]STVSLTTK	CPTAC-1301: QPCT. YFQN[+1.0]YSYGGVIQDDHIPFLR	CPTAC-1302: CA1. EIINVGHSFHVNFEDNDN[+1.0]R
CPTAC-1303: VIM. QDVDN[+1.0]ASLAR	CPTAC-1304: DPP7. N[+1.0]LSASVIAVTIQGGAHHLDLR	CPTAC-1305: SERPING1. M[+16.0]LFVEPILEVSSLPTTN[+1.0]STTNSATK
CPTAC-1306: CEACAM8. LFIPN[+1.0]ITTK	CPTAC-1307: DSG2. ESFLAPSSGVQPTLAM[+16.0]PNIAVGQN[+1.0]VTVTER	CPTAC-1308: M6PR. LKPLFN[+1.0]K
CPTAC-1309: ACP2. FNPN[+1.0]ISWQPIPVHTVPITEDR	CPTAC-1310: PCDH1. AN[+1.0]DSDQGANAEIEYTFHQAPEVVR	CPTAC-1311: ITGB8. NYAIIKPIGFN[+1.0]ETAK
CPTAC-1312: CALCOCO2. SAN[+1.0]STLAQALHWTR	CPTAC-1313: MINPP1. N[+1.0]ATALYHVEAFK	CPTAC-1314: KRT40. TYN[+1.0]ASVLPVR
CPTAC-1315: MMP23B. ALM[+16.0]HLN[+1.0]ATLR	CPTAC-1316: CD6. EGDN[+1.0]ITLK	CPTAC-1317: CD6. N[+1.0]ATVVWM[+16.0]K
CPTAC-1318: CD6. TVNSLN[+1.0]VSAISIPEHDEADEISDENR	CPTAC-1319: IRAK2. TILVDN[+1.0]NTWNNTHISR	CPTAC-1320: AEN. LN[+1.0]STAQK
CPTAC-1321: PLTP. N[+1.0]WSLPNR	CPTAC-1322: OVGP1. FVPIYGN[+1.0]HSSVNSVTPQTSPLSLK	CPTAC-2612: MMP23B. ALM[+16.0]HLN[+1.0]ATLR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	21.4	8.2	6.7	24	8.9	7.1	32.2	12.1	9.8	15	15	15
y7 (1+)	64.6	14.4	14.1	59	14.3	15.1	87.5	20.3	20.7	15	15	15
y13 (2+)	83.2	14.7	18.8	74.9	15.4	18.7	111.9	21.3	26.5	15	15	15
sum	22.9	4.9	8	25.5	6.4	7.3	34.3	8.1	10.8	15	15	15

ITGB8

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1311 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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ITGB8

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1311 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments